Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,489,775	C→T	V97M (GTG→ATG)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,489,775	0	C	T	100.0%	116.7 / NA	35	V97M (GTG→ATG)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/12);  total (23/12)

CTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAAC  >  NC_000913/4489534‑4490019                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACTCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCCACGGATGGCCTTTCTCGCCAAAGATATCCCACATCCCCACCgg                                                                                                                                                                                                                                             >  2:95627/1‑251 (MQ=255)               gTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGtt                                                                                                                                                                                                                               >  1:116071/1‑251 (MQ=255)                  cTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGcc                                                                                                                                                                                                                            >  1:373984/1‑251 (MQ=255)                    gATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCa                                                                                                                                                                                                                           <  2:3981/250‑1 (MQ=255)                              ggATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGAGGGCCTTTCTCGCCAAAGCTATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGc                                                                                                                                                                                                                >  1:367733/1‑251 (MQ=255)                               gATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGcc                                                                                                                                                                                                               >  1:127550/1‑251 (MQ=255)                                 tttGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGCGCAGTAACAGCCCCTGATCGTCAGCCATGCGGAAGATGATATTCAGCACGCCAGATTGCACCTCGTTGAGATTCACCAGTCGTGCAAGCAACAGCAGCCCCAGATCCGACACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGGTATCCCACCTCACCCCCGGATTGGCATGCGGTTGCCAGTCTTTGACGCCgt                                                                                                                                                                                                             >  1:143726/1‑250 (MQ=255)                                         tATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAttttt                                                                                                                                                                                                      <  1:127229/250‑1 (MQ=255)                                            cGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATGTTTAAg                                                                                                                                                                                                   <  1:323673/250‑1 (MQ=255)                                                gATGTACTGGGTAATTGCCAGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTAGCCCGCACCGGATGGCCTTTCTCGCCCAAGATATCCCACATCCCCACCGGCTTGACATGCGGTTGCCCGACATTTACGCCGGTCTTTTTCAGCCtcg                                                                                                                                                                                              >  2:196977/1‑249 (MQ=255)                                                                gCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGCTTGCAAATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAACGATCTCCCACCTCCCCACCGGATTGGCATGCGGTTGCCAGTGATTTACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCg                                                                                                                                                                               >  2:374615/1‑250 (MQ=255)                                                                            ttAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGACACCCTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTcctgc                                                                                                                                                                  >  1:362034/1‑251 (MQ=255)                                                                                aGTCGAGCAGTAACCGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCAGAAACCGTCGCCCGCCCCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGATGCCAGTCATTGACGCCGATATTTTTAAGACTTGCGAGCAGTTTTTCCGACACCGTTCctgcctgc                                                                                                                                                              >  1:71510/1‑251 (MQ=255)                                                                                      gCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACg                                                                                                                                                        <  1:60033/251‑1 (MQ=255)                                                                                                           cAGCAATGCGGAAGATGATATTCAGCACGCCAGAGTGCGCATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTGCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTcaca                                                                                                                                   <  1:249716/251‑1 (MQ=255)                                                                                                                    ggAAGATGATATTCAGCCCGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCACAAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATGGGCATGCGGTTGCCAGTCATTGAAGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCGGGTCAGATCGCCTTTCACATCAGCCAt                                                                                                                           >  1:126499/1‑250 (MQ=255)                                                                                                                            atatTCAGCACGCCAGATTGCACATCGGTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCATGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCgg                                                                                                                  >  1:317775/1‑251 (MQ=255)                                                                                                                              atTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGAAGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGc                                                                                                                 >  1:339557/1‑250 (MQ=255)                                                                                                                                         cAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTcc                                                                                                     >  1:346026/1‑251 (MQ=255)                                                                                                                                          aGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCg                                                                                                    >  1:372395/1‑251 (MQ=255)                                                                                                                                                 acaTCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGGTCAGAAACCGTCGCCCGCACCGGCTGGGCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATCCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGCGCCGTTTTTCCTACCCCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCtt                                                                                                                                        >  1:48720/1‑208 (MQ=255)                                                                                                                                                     cGTTGGGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGACACCGTCGCCCGCACCGGAGGGCCTTTCTCGCCAAAGATACCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAGTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGCCAATGACTcc                                                                                         <  2:74889/251‑1 (MQ=255)                                                                                                                                                     cGTTGAGATTCAACAGTCGTGCCAGCAACATCGGCCCCAGATCCGAAACCGTCGCCCGCACCGTATGGCCTTGCTCGCCAAAGATAGCCCACATCACCACCGGATTGGCATGCGGTTGCCTGTCATTGACGCCGATATTTTTAATCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTcc                                                                                         <  1:218321/251‑1 (MQ=255)                                                                                                                                                                  cAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGTCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCa                                                                            <  2:2324/251‑1 (MQ=255)                                                                                                                                                                  cAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAAACGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGACGATATTTTTAAGACTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGACATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGc                                                                             >  1:191873/1‑250 (MQ=255)                                                                                                                                                                      cGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTGTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCGTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGt                                                                        <  1:20807/251‑1 (MQ=255)                                                                                                                                                                         gCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAAc                                                                     >  1:281589/1‑251 (MQ=255)                                                                                                                                                                               aaCAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGtttt                                                               <  1:74269/251‑1 (MQ=255)                                                                                                                                                                                   gCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTAccc                                                           <  1:12724/251‑1 (MQ=255)                                                                                                                                                                                                cGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTgcgc                                              >  1:41638/1‑251 (MQ=255)                                                                                                                                                                                                  aaaCCGCCGCCCGCACCGTATGACCTTGCTCGCCAAAGATATCCCCCATCACCGCCGGATTGGCATGCGTTTGCCAGGCATTGACTCCGAGTTTTTTAAGCCTTGCTAGCAGTTTTGCCGACACCTTTCCTGCCTGCGCGACGCCGGTCATATCCCGTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCCGCCCCGTTGCGCCg                                            <  2:376811/251‑1 (MQ=255)                                                                                                                                                                                                                            ttCTCGCCAAAGCTATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGACAt                  >  2:29377/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaaGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGc           >  1:96178/1‑250 (MQ=255)                                                                                                                                                                                                                                           tCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTaaaa    >  1:112537/1‑250 (MQ=255)                                                                                                                                                                                                                                              cacaTCACCACCGGCTTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGACGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTCAAAAc  >  2:381055/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAAC  >  NC_000913/4489534‑4490019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑