Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,457,392	C→T	E158E (GAG→GAA)	yjgA ←	putative ribosome biogenesis factor YjgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,457,392	0	C	T	100.0%	157.1 / NA	47	E158E (GAG→GAA)	yjgA	putative ribosome biogenesis factor YjgA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/21);  total (26/21)

CCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAC  >  NC_000913/4457148‑4457636                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ccGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCACACCATACTCACATCACCAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTtctttttctttc                                                                                                                                                                                                                                                >  2:321716/1‑251 (MQ=255)   gcgcAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTtctttctt                                                                                                                                                                                                                                              <  1:61464/251‑1 (MQ=255)      cAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTcgc                                                                                                                                                                                                                                           >  1:281461/1‑251 (MQ=255)                             ataGTATGCTTAGGCCGGATAAGGGTTTCACGCCGCAGCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTTGCGTGCGGATTTCGGAGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGtt                                                                                                                                                                                                                    <  2:334082/251‑1 (MQ=255)                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCGTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCgg                                                                                                                                                                                                        <  1:252761/251‑1 (MQ=255)                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGc                                                                                                                                                                                                       <  1:214596/251‑1 (MQ=255)                                             ggATAAGGCGTACACGCCGAATCAGGCATATCCGCATAGTGCCTGATGAGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCACACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTTCGGATCAGAGTACGCAGTTGCTGACGCTCGGCATc                                                                                                                                                                                                    >  2:21409/1‑251 (MQ=255)                                              gATAATGCGTTCAAGCAGCATCCGGCATTTGAGCATAGTGCCTCATGCGACGCTTGATGCGCCTTATCATGCCTACAGGAGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTAGTTCTCCGCTAACTCGCGCAGATACTGGAACATCTGGCGTGCGGATTTCGGCGGCTTATACCCTTCTTTTTCTTTCTTCGCGTTGCGGATCTGCGTCCGCAGTTGCTGACGATCGGCagcg                                                                                                                                                                                                   >  2:286340/1‑248 (MQ=255)                                                tAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCggg                                                                                                                                                                                                 >  2:59326/1‑251 (MQ=255)                                                             ccgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAAt                                                                                                                                                                                    <  2:238042/251‑1 (MQ=255)                                                             ccgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAAt                                                                                                                                                                                    >  1:314533/1‑251 (MQ=255)                                                               gcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTCTCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGCTCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATAc                                                                                                                                                                                  >  1:266028/1‑251 (MQ=255)                                                               gcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATAc                                                                                                                                                                                  >  2:215593/1‑251 (MQ=255)                                                                        aTTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGAt                                                                                                                                                                         >  1:325313/1‑251 (MQ=255)                                                                            gAGCATAGTGCCTGATTCGACGCTTGATGCGTCTTATCAGGACTACAGGTGCACCGTATCCGGCAAACCATACACACATCACCAACGAAAATTACCCTGCGTTCGCCGCTAACTCGCGCAGATACTGGAAAAGCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCGTCGCGTTGAGGATCAGAGTACGCAGTTGCTGACGATAGGCATCGGGCCACAGATTTAATCCCTCAGCGATGGc                                                                                                                                                                      <  2:286238/250‑1 (MQ=255)                                                                             aGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGt                                                                                                                                                                    <  1:316112/251‑1 (MQ=255)                                                                              gCATACTGCATGATGCGCCGCTTGACGCGTCTTATCCCGCCTCCAGGTGAACCGTATCCGGCAAACCATACTCCAATCAACAACGAAACTTAACCTTCGTTATCCGCTAACTCGCGCAGATACTGGCAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCCCGTTCCGGATCAGAGTACGCCGTTGCTGCCGATCGGACTCGGGCCACAGATTTACTACCACAGCAATGGCGt                                                                                                                                                                    >  2:376576/1‑250 (MQ=255)                                                                                aTAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGtcat                                                                                                                                                                 >  1:122730/1‑251 (MQ=255)                                                                                  aGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGtcatc                                                                                                                                                                >  1:72524/1‑250 (MQ=255)                                                                                      ccTGATGCGACGCTTGATGCGTCGTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCtt                                                                                                                                                           >  1:94873/1‑251 (MQ=255)                                                                                         gATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTga                                                                                                                                                         <  1:68714/250‑1 (MQ=255)                                                                                          aTGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTgatc                                                                                                                                                       <  1:11346/251‑1 (MQ=255)                                                                                                     gATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCTTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGtt                                                                                                                                                                                                                                        <  2:338652/159‑1 (MQ=255)                                                                                                      aTGCGTCTTATCAGGCCTACCGGAAGACAGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACCCGCGCAGATACTGGAAACTCTGGCGTGCAGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCCGATCAGAGTACCCAGTTGCTGACGATCGGCATCGGGACACAGATTTACTACCTCAGCGCTGGCGTCCTCACCTTGATCGATCAGACgctc                                                                                                                                           >  2:85926/1‑248 (MQ=255)                                                                                                            cTTATCAGGCCTACAGGCGCACCGTATCAGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGCTTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGTTCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACATTGATCGATCAGACGTTCgcgc                                                                                                                                       >  2:181137/1‑249 (MQ=255)                                                                                                                  aGGCCTACAGGTGCACCGTATCCGGCAAACCATAATCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGCTTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTc                                                                                                                               >  1:149336/1‑251 (MQ=255)                                                                                                                     tttacgatctcACCGGATCCGGCAAACCATACTCACAGCAACAACGAAAAGTACCCTTCGTGCTCCGATAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTGTTCTTTCTTCGCGTGGCGGATCAGAGTACGCAGTGGCGGACGATCGGCATCGGGCCACAGATTTAAGACCGCAGCGATGGCGTCATCACCGTTATCGATCAGACGATCGCGCAGGtttgcc                                                                                                                              <  2:253677/239‑4 (MQ=255)                                                                                                                      cTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGt                                                                                                                           >  1:116251/1‑251 (MQ=255)                                                                                                                          aGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTAt                                                                                                                       <  1:373873/251‑1 (MQ=255)                                                                                                                           ggTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAACATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTACGGATCAGAGTACGCCGTTGCTGACGATCGGCATCGGGCCCCAGATTTAATACCTCAGCGCTGGCTTCCTCACCTTGCTCGATCAGACAATCGCGCAGGTTTTCCAGTTTATg                                                                                                                      >  1:56280/1‑251 (MQ=255)                                                                                                                               cACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTcc                                                                                                                              >  1:253677/1‑239 (MQ=255)                                                                                                                                  cGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGCTACTGGAACATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGCTGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGc                                                                                                               >  1:390764/1‑251 (MQ=255)                                                                                                                                   gTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGca                                                                                                              <  1:369903/251‑1 (MQ=255)                                                                                                                                            aaaCCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTtctttttctttc                                                                                                                                                                                                                                                >  2:390749/1‑112 (MQ=255)                                                                                                                                            aaaCCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTtctttttctttc                                                                                                                                                                                                                                                <  1:390749/112‑1 (MQ=255)                                                                                                                                              aCCATACTCACATCAACAACAAAAACTACCCTTAGTTCTCCGCTAACCCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATTGAACAGCACCACCTGCTgg                                                                                                   >  2:231201/1‑251 (MQ=255)                                                                                                                                                            aacaacGAAGAATACCCTTCGTTCTCCGCTACCTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGATTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGCGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTCTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGttttt                                                                                     >  2:54627/1‑251 (MQ=255)                                                                                                                                                                  gAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTGCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCtt                                                                               <  1:362376/251‑1 (MQ=255)                                                                                                                                                                               cGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTg                                                                   <  1:215593/250‑1 (MQ=255)                                                                                                                                                                                              cgcgCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTAGCCAGCGCCTGACGAATAGGCTCTAc                                                    <  2:141140/250‑1 (MQ=255)                                                                                                                                                                                                                   ggCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCtt                               >  2:234192/1‑250 (MQ=255)                                                                                                                                                                                                                   ggCGTGCGCATTTCGGCGGCTTATACCCAACTATTTCTTTCTTCGCGTTGAGGCACAGAGTAAGGAGTTTCTGACGATCGGCATCGGGCCCCAGCTTTAATACCTCAGCGATGGCGTCATCCCCTTGATCGATCCGACGATCGCGTCTGTTTTCCAGTTTATGGACCAGCACCACCTGCTGGTTGTGACGGTTTTTCAACTTATCCCGCGACAGACGAATAGGCTCTACGTCCCGCAGGCGCACCATCttc                              >  2:376704/1‑250 (MQ=255)                                                                                                                                                                                                                    gCGTGCGGAGTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATATTAc                             <  1:214063/251‑1 (MQ=255)                                                                                                                                                                                                                      gTGCGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCg                           <  1:234192/251‑1 (MQ=255)                                                                                                                                                                                                                         cGGATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGCTTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCgctg                        >  1:161959/1‑248 (MQ=255)                                                                                                                                                                                                                          ggATTTCGGCGGCTTATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATg                        >  1:219567/1‑250 (MQ=255)                                                                                                                                                                                                                          ggATTTCGGCGGCTTATTCCCGTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGa                       <  1:88394/251‑1 (MQ=255)                                                                                                                                                                                                                                     gattATTCCCATCTTTTTCTTTCATCACGTTGCGGATCAGAGTACGCAGTTTCTGACGATCGGCATCGGTCCACAGCTTTAATCCCTCAGCGATGGCGTCATCCCCTTGATCGATCAGAAGATCGCGCAGGTTTTCCAGTTTATGGACCAGCACCACCTGGTGGTTGTGACGGTTCTTCAGCTTATCAAGCGCCTGACGCATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGCTGAGCTGCAGCTgg            >  2:265934/3‑251 (MQ=255)                                                                                                                                                                                                                                        tATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcg          <  2:149336/250‑1 (MQ=255)                                                                                                                                                                                                                                        tATTCCCTTCTTTTTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggc           <  2:325313/249‑1 (MQ=255)                                                                                                                                                                                                                                                tctttttctttcTTCGCGTTGCGGATCAGAGTACTCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCATGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCATCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAc  <  1:169381/250‑1 (MQ=255)                                                                                                                                                                                                                                                tctttttctttcTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAc  <  1:369740/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAC  >  NC_000913/4457148‑4457636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑