Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,418,862	C→T	G255D (GGT→GAT)	ulaG ←	L‑ascorbate‑6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,418,862	0	C	T	100.0%	160.9 / NA	50	G255D (GGT→GAT)	ulaG	L‑ascorbate‑6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/25);  total (25/25)

ACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTT  >  NC_000913/4418615‑4419108                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aCCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATcgc                                                                                                                                                                                                                                                     >  1:111871/1‑251 (MQ=255)    gcgcgGATAGTGGTACTCGATGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAACATATCGGCGCTGGTCATTTTGTCGGTGATATcgcgcg                                                                                                                                                                                                                                                  >  2:213426/1‑251 (MQ=255)       cggatagtacaaCTCGAAGTTGTCTCTATCCAGCGGCCAGGTAACTTTGCAAACAACATGCCAGCTAAACGGCTTGAAGCCATACTTCCGGCGATCTTTTTTCATCTCCAACAGCACGCGGCTCTATTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATCTCGGCGCTGGTCATTTTGTCGGTGCTATCGCGCGGGt                                                                                                                                                                                                                                               >  2:182167/12‑251 (MQ=255)         gATAGTTGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCCCCTGCCAGATAAACGGCTTGAAGCCATACCTCAGGCGATCTTGTGTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCTCCCATACGCAGCATATCGGCGCTGGTCATTTTTTCTGTGATATCGCGCGGGttt                                                                                                                                                                                                                                             <  2:350695/251‑1 (MQ=255)                  aCTCGAAGTTGTCTGTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTGCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTGTGGCCAGATATCGTTGTGGAACGGGATCACTACTTTCGCATTCAGCGCTGCACCCATACGCAGCAGATCGGCGCTTGTCATGGTGTCGGGTATATCGCTGGGGTTGTCGCCGTAc                                                                                                                                                                                                                                    <  2:208181/251‑1 (MQ=255)                   cTCGAAGATGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCCCAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACg                                                                                                                                                                                                                                   >  2:327953/1‑251 (MQ=255)                            gTCTTTATCCAGCGGCAAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGCAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACg                                                                                                                                                                                                                          >  2:337107/1‑251 (MQ=255)                                ttATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCa                                                                                                                                                                                                                       <  2:111871/250‑1 (MQ=255)                                 tATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACg                                                                                                                                                                                                                     >  1:221262/1‑251 (MQ=255)                                 tATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGACCCTAACGCCAc                                                                                                                                                                                                                      <  1:213426/250‑1 (MQ=255)                                     cAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGa                                                                                                                                                                                                                                  >  1:233963/1‑234 (MQ=255)                                     cAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGGCGGTGATATCGCGCTGGTTTTCGCCGTACGa                                                                                                                                                                                                                                  <  2:233963/234‑1 (MQ=255)                                            cAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATg                                                                                                                                                                                                          >  1:155836/1‑251 (MQ=255)                                                                     aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCataat                                                                                                                                                                                   >  2:5850/1‑249 (MQ=255)                                                                            cGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGagt                                                                                                                                                                          >  2:106584/1‑251 (MQ=255)                                                                               cTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGagtagt                                                                                                                                                                       >  1:241215/1‑251 (MQ=255)                                                                                   aaGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATAGCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTGAGAGTAGTGGGa                                                                                                                                                                   <  1:271995/251‑1 (MQ=255)                                                                                        aTACTACAGGCGCTCTTTTTTCATATCCCACAGAACGCGGATCTCTTGCGGATCGGCCTGGATGTTTGACCAGATATCGTGGTGGAACGGGATCACTAAATTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGATGGTCATTTTGTCTGTGATATCGCGCGGGTTTTCGCCGTACGATCATAGAGCAACGTCGAGCTGATGTTCGTTCCCGGGCTTCGCATGATAGTTAGAGTAGTGGGAGTcgc                                                                                                                                                              >  2:201695/1‑251 (MQ=255)                                                                                          aCTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgcc                                                                                                                                                             <  2:43372/250‑1 (MQ=255)                                                                                           cTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGCTCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTcgccgc                                                                                                                                                           >  2:188762/1‑251 (MQ=255)                                                                                             tCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCat                                                                                                                                                                                      >  1:254356/1‑222 (MQ=255)                                                                                                        tttttCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCGTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATAc                                                                                                                                                   <  2:141163/246‑1 (MQ=255)                                                                                                            tCATCTCCCACAGTCCGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATAGCGTGGGGGAACGGTATCGCTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTTATGTTCGTTACCTTTCTTCGCATAATATTTAGAGTAGTTTGAGTCGCCGCTGTGATACAGTGAgac                                                                                                                                           <  2:269802/250‑3 (MQ=255)                                                                                                               tctcCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAgccgcc                                                                                                                                        >  1:39812/1‑250 (MQ=255)                                                                                                                        gCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTg                                                                                                                              <  1:293081/251‑1 (MQ=255)                                                                                                                          aCGCGGATCTCTTGCGGATCGGCATGGAAGGTTGACCAGATATCGTGGTGGAAAGGGATCACTAATTTCGCATTTAGCGCTTCCCCCATACGCAGCATATCGGCGCTGGTCATTTTGGCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGa                                                                                                                             >  2:293080/1‑250 (MQ=255)                                                                                                                                        cGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACcg                                                                                                              <  2:72802/251‑1 (MQ=255)                                                                                                                                          gATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACcgcg                                                                                                            <  1:247563/251‑1 (MQ=255)                                                                                                                                                   ggAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTcc                                                                                                   <  1:46590/251‑1 (MQ=255)                                                                                                                                                       gTTTGACCCGATATCGTGGTGGAACGGGATCCCTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGc                                                                                               <  2:221262/251‑1 (MQ=255)                                                                                                                                                             ccAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTGTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGTTAGTTCACCGCGCGATCGTCCATGCCATCTg                                                                                         <  2:169197/251‑1 (MQ=255)                                                                                                                                                              cAGATACCGTGGTGTAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGACGGTTATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCTCGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTCGAGTAGTGGGAGTCGCCGCTGTGCTACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGCTCGTCCATGCCATCTgg                                                                                        <  2:93231/251‑1 (MQ=255)                                                                                                                                                                              aCGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCTCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTCCCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTTGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCt                                                                        <  1:268205/251‑1 (MQ=255)                                                                                                                                                                                gggATCACTACATTCGCATTCAGCGCTTCACCCATCCGCAGCATATCGACGCTGGTCATTTAGTCGGTGATCTCGCGCGGGTTTTTGCCGTACGATCCTAGCGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGCGAGAGTCTCCGCTGTGATACAGGGAGCCGCCAGGCCTTTTGAACAGGCAGTTCACCGCGCGATCGTCCATGCCATCTGGCTGGACGCCAGCCGCttt                                                                      >  2:336453/1‑251 (MQ=255)                                                                                                                                                                                  gATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCttttt                                                                    >  1:202769/1‑251 (MQ=255)                                                                                                                                                                                        tactTTCGCATTCAGCGCTTCACCCATACGCAGCATATTGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCgg                                                              >  1:396232/1‑251 (MQ=255)                                                                                                                                                                                        tactTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTAAGCCAGCCGCCTTTTGATCgg                                                              >  2:43112/1‑251 (MQ=255)                                                                                                                                                                                         actTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCggc                                                             >  1:203448/1‑251 (MQ=255)                                                                                                                                                                                                    cagcgtgtCACCCATCCGCAGCATAGCTGCGCTGTTCATGTTGTCGGTGCTATCGCGCGGGTTTTCGCCTTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCTCTTAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACACGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCTCCGCGCGATCGGCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTCATCGGCAGGCAGGGTGa                                                  <  2:130877/244‑1 (MQ=255)                                                                                                                                                                                                           tCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGc                                           >  1:140015/1‑251 (MQ=255)                                                                                                                                                                                                            cACCCATACGCATCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTTGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGc                                           <  1:258564/250‑1 (MQ=255)                                                                                                                                                                                                                  tACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACgg                                    >  1:150501/1‑251 (MQ=255)                                                                                                                                                                                                                    cGCAGCATAGCGGCGCTGGTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATTGTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCAGCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTc                                  <  2:49207/251‑1 (MQ=255)                                                                                                                                                                                                                                     ggTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCa                                                                                      <  2:153193/182‑1 (MQ=255)                                                                                                                                                                                                                                     ggTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCa                                                                                      >  1:153193/1‑182 (MQ=255)                                                                                                                                                                                                                                     ggTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCAt                 <  1:69935/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTCATTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGTGAGCCGCCAGGCGTTTTGAACAGGTAGTTCCCCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATg                <  1:3975/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTCAGTTTGTCGGTGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATg                <  1:159931/251‑1 (MQ=255)                                                                                                                                                                                                                                                   tGATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAAAAGTTAGAGTAGTGGGAGTAGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGAAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCt   >  1:173239/1‑251 (MQ=255)                                                                                                                                                                                                                                                    gATATCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCtt  <  1:402896/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCAGCGCTTCACCCATACGCAGCATATCGGCGCTGGTCATTTTGTCGGTGATACCGCGCGGGTTTTCGCCGTACGATCCTAACGCCACGTCGATCTGATGTTCGTTACCGTGCTTCGCATAATAGTTAGAGTAGTGGGAGTCGCCGCTGTGATACAGGGAGCCGCCAGGCGTTTTGAACAGGTAGTTCACCGCGCGATCGTCCATGCCATCTGGCAGTACGCCAGCCGCTTTTTGATCGGCAGGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTT  >  NC_000913/4418615‑4419108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑