Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,469	C→T	E17K (GAA→AAA)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,469	0	C	T	100.0%	224.8 / NA	67	E17K (GAA→AAA)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

TCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAG  >  NC_000913/4380223‑4380713                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     tCGGGAAGTTAGCTAACGCTTAAACCCACATCCCGACCGAGCAATAACGAACGAAGGCATTCCATCCCAGTATTGCCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGCTCGCTCAGGTCCCGTCCCAGGTTGTCTCTGATGTAGCCCAGCGAATCCCGTAATGGGGTAGTTGCGTCATATGGCACTTCATAGAATGAGCCCTGCGGTGCGGCATCGACTTTCggg                                                                                                                                                                                                                                                  >  2:359525/1‑251 (MQ=255)     gAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTTTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTtat                                                                                                                                                                                                                                              <  2:256088/251‑1 (MQ=255)              tAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGcacc                                                                                                                                                                                                                                      >  1:119911/1‑250 (MQ=255)                     tCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCa                                                                                                                                                                                                                                <  1:73842/249‑1 (MQ=255)                      cAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCCGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTATTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGAACCACCTCAAtt                                                                                                                                                                                                                             >  1:256159/1‑251 (MQ=255)                        cccTTCATACCGTCGGTGGAATCACGCAGGGAGGTTTTTCATGCCAGTTTTTGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCACGTCCGGTGCCAGTGTGTCTTTGATGTAGCCCAGCGCATCCAGTATTTAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAGTCGGTGCGGTATCGACTTTCGGTTTATAGCGCACCTCCTCAAgttt                                                                                                                                                                                                                           <  2:256159/250‑1 (MQ=255)                          cTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGAATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTc                                                                                                                                                                                                                          >  1:69105/1‑250 (MQ=255)                             cATACCGTCGGTGTAATCCCGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGt                                                                                                                                                                                                                      <  2:301905/251‑1 (MQ=255)                               tACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGATCAGGTCCGGAGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCACTTTTCAGGttt                                                                                                                                                                                                                    >  1:20730/1‑251 (MQ=255)                                aCCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGCTGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATTCGGTGCGGTATCGACTTTCGGGTTATAGCGCACAACCTCAATTTTCAGGtttt                                                                                                                                                                                                                   >  2:314155/1‑251 (MQ=255)                                 ccGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGttttt                                                                                                                                                                                                                  <  1:13607/251‑1 (MQ=255)                                   gccGGTGTACTAACGCAGGCAGGTTTTACATGCCAGTTTTGGCACGCTGTTAACCAACATGCCGCATGAACCTCAAATCGCCATACGGCTGGACCACCGGTAGCTCAGGTCCGGTGCCCGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTACTGAGGCAGTTCCGTCATAAGGCTCTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATATCGCTACACCTCAATTTTCAGGTTTTTCa                                                                                                                                                                                                                <  2:385315/249‑1 (MQ=255)                                     cGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTGTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAt                                                                                                                                                                                                               <  1:139165/250‑1 (MQ=255)                                      ggTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtct                                                                                                                                                                                                             <  1:89398/251‑1 (MQ=255)                                         gtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAg                                                                                                                                                                                                          <  1:331945/251‑1 (MQ=255)                                            aTCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGACATACGGCAGGAACAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGcc                                                                                                                                                                                                        >  1:244887/1‑250 (MQ=255)                                               ccGCAGGAAGGTTTTACATTCCAGTGTTGGCACGTTGTTCACCATCATGCCGCAGGAACCCCAAATCGCCATACGGCAGGACCAGCGGTAGCTCATGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTc                                                                                                                                                                                                    <  2:245137/250‑1 (MQ=255)                                                  cAGGAAGGTTTTACATGCCAGTTTTAGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGTCCAGCGGTAGCTCAAGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGCAATGAGGAAGTTGAGTCATAAGGCACTTCATAGCATGCGCTATGCGGTGCGGTATCGACTTTCGGGGTATAGCGCACCACCTCACTTTTCAGGTTTTTCCTCTCAGCCATTCGcc                                                                                                                                                                                                 >  2:237785/1‑251 (MQ=255)                                                   aGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCTGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCTCTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcct                                                                                                                                                                                                <  1:399426/251‑1 (MQ=255)                                                    ggAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcctt                                                                                                                                                                                               <  1:399663/251‑1 (MQ=255)                                                     gAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTGTCAGGGTTTTCAGCTCAGCCATTCGccttc                                                                                                                                                                                              <  2:183020/251‑1 (MQ=255)                                                        ggTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGAACAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctcc                                                                                                                                                                                           >  1:186756/1‑251 (MQ=255)                                                         gTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctcct                                                                                                                                                                                          >  1:275957/1‑251 (MQ=255)                                                               cATGCCAGTTTTGTCCCGTTGCTAAACATAATGCAGCAGGAACCACCAATCGCCATACGGCAGCACCCGCGGTCGCTCAAGTCCGGAGCCAGGCTGTCTTTGATGCAACCCCGAGCATCCAGAAATGCGGTGGCTGCGTCATAAGGCACCTCATAGAATGCGCTATGCGGTGCGGTATCGAATTTCGGGTTATAGCGCCCCACCTCACTTTTCAGGTTTTTCTTCTCAGCCCTTCGCCTTCTCCTTCTTAt                                                                                                                                                                                    >  2:169246/1‑251 (MQ=255)                                                               cATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCa                                                                                                                                                                                                                                >  1:344802/1‑207 (MQ=255)                                                                                 tagttAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGAACAGCGGTAGCTCAGGTACGGAGACAGGTTGACTTTGATGATGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGTCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTAGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCATCTCCTTCTTATTGGCTGCTTCCGCCTTAt                                                                                                                                                                  >  2:323387/3‑251 (MQ=255)                                                                                            catGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATcc                                                                                                                                                       >  1:15904/1‑251 (MQ=255)                                                                                                  gCAGGAACCACAAATCGCCATACGGCAGGACCCGCGGTAGCTCAGGGCCGGTGCCAGGCTGCCTTTGATGTAGCCCAGCGCATCCAGTCATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTTCTTCCGCCTTATCGGCTGCATCCGCTTcc                                                                                                                                                 >  2:176727/1‑250 (MQ=255)                                                                                                    aGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGAGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATTCGCTTCGcc                                                                                                                                               >  2:102271/1‑251 (MQ=255)                                                                                                       aaCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGACCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCAcc                                                                                                                                            >  1:14327/1‑251 (MQ=255)                                                                                                            taaaTCGGCATACGGCAGGAACAACGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGAAGTAGCCCAGCGCATCCAGTCATGAGGCAGTTGCGTCATAAGGCACTTAATAGAATGCGCTATGCGGGGCGGTATCGACTTTCGGGGTATCGCGCACAACCTCCATTTTCAGGTTTTTCATTTCAGCCATTCGACTTCTACTTCTTATTGGCTGCTGCCGCTTTATGGGCTGCATCCGCGTCGCCCCCGTaa                                                                                                                                        >  2:331240/2‑250 (MQ=255)                                                                                                             aaaTCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTTCATCCGCTTCGCCACCGTAAAc                                                                                                                                      >  2:10589/1‑251 (MQ=255)                                                                                                              aaTCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCTGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCATGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCGTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAcg                                                                                                                                     <  2:166958/251‑1 (MQ=255)                                                                                                                tCGCCATCCGGCAGGACCCGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAagcg                                                                                                                                   >  2:269407/1‑251 (MQ=255)                                                                                                                 cGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAcgcg                                                                                                                                   <  1:169391/250‑1 (MQ=255)                                                                                                                  gCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGtt                                                                                                                                 >  1:318751/1‑251 (MQ=255)                                                                                                                     aTACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAg                                                                                                                              <  1:562/251‑1 (MQ=255)                                                                                                                           cAGGACCAGCGTTAGCTCAGGTCCGGTGCCAGGTTGTCTTGGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGGTATAGCGCACCACCTCAATTTTCAGGGTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTAtt                                                                                                                                                                                   <  2:369867/192‑1 (MQ=255)                                                                                                                           cAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTAtt                                                                                                                                                                                   >  1:369867/1‑192 (MQ=255)                                                                                                                                  aGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgt                                                                                                                 <  1:220813/251‑1 (MQ=255)                                                                                                                                      ggagcgcAGGTCCGGTGCCCGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCCCCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATccc                                                                                                                                                      <  2:7988/204‑2 (MQ=255)                                                                                                                                      gTAGCTCAGCTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGAGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCg                                                                                                                                                      >  1:7988/1‑210 (MQ=255)                                                                                                                                             aGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCa                                                                                                      <  1:349429/251‑1 (MQ=255)                                                                                                                                             aGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCa                                                                                                      <  2:275957/251‑1 (MQ=255)                                                                                                                                                  cGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCg                                                                                                 >  1:69291/1‑251 (MQ=255)                                                                                                                                                  cGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATACGCCTTCTCCTTCTTATTGGCTGCTTACGCCTTATCGGCTGCATCCGCTTCGCCAACGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCg                                                                                                 >  1:360647/1‑251 (MQ=255)                                                                                                                                                             tGTCTTTGATGTAGCCCAGCGCAGCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCTGTGCTGTATCTACTTTCGGGTTAGAGCGCACCACCTCAATTTTCAGGGTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTTGCTGCGTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTGTAGCTGGCGGCAGCGTAGTAATCTTCACGTCTCTGTACTCCa                                                                                       <  2:308571/250‑1 (MQ=255)                                                                                                                                                              gTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAg                                                                                      <  2:99775/250‑1 (MQ=255)                                                                                                                                                                   tGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAg                                                                                 <  1:212663/250‑1 (MQ=255)                                                                                                                                                                     aTGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCAc                                                                                                     >  1:294903/1‑228 (MQ=255)                                                                                                                                                                     aTGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCGTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCAc                                                                                                     <  2:294903/228‑1 (MQ=255)                                                                                                                                                                            ccAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATc                                                                       >  1:136092/1‑251 (MQ=255)                                                                                                                                                                                 gcATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGATTCGTGCCATCAGCAt                                                                  <  1:83162/251‑1 (MQ=255)                                                                                                                                                                                             gAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCgg                                                      >  2:322868/1‑250 (MQ=255)                                                                                                                                                                                             gAGGGAGTTGCGTCATAAGGTACTTCATAGAAGGCGCTAGGCTGAGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTGTCATCTCAGCCATTCGCCTTCTTCTTCTTATGGGCGGCTTCCGCCTGATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGGCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGATGGCGa                                                      <  2:206672/251‑1 (MQ=255)                                                                                                                                                                                               ggTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGGGTCGTGCCATCAGCATCGCGGAAGGCGAgg                                                    >  2:85460/1‑251 (MQ=255)                                                                                                                                                                                                     tGCGTCATAAGGCACTTACTAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCACCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTAGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGgtgtgt                                              >  1:117029/1‑251 (MQ=255)                                                                                                                                                                                                        gTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTg                                           <  2:359365/251‑1 (MQ=255)                                                                                                                                                                                                          cATAAGGCACTTCATAGAATGCGCTATGCGCTGCGGTATCGACTTTCGGGTCATAACGCACCACCTCAATTTTACGGCTTTTCATCTCAGACATTCGACTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGCAGGCGAGGGTGTGTTTg                                           >  2:58774/1‑249 (MQ=255)                                                                                                                                                                                                                gCACTTCATAGAATGCGCTACTCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCATCCATTCGCCTTCTCCGTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGTAGCGTAGTAAGCTTCACGTCGCTGTACTCCAGGCGAGTCTTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGtt                                   <  1:242555/251‑1 (MQ=255)                                                                                                                                                                                                                 cACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTg                                  >  2:350165/1‑251 (MQ=255)                                                                                                                                                                                                                  aCTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTg                                  >  1:376197/1‑250 (MQ=255)                                                                                                                                                                                                                      cATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCTCACCACCTCAATTTTCAGGGTTTTCATCTCAGCCATTCTCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCGTCGCCACCGTAAACGCGGTGAGCTGGCGGCAGCGTAGTAATCTGCACGTCGCTGTTCTCCAGGCGAGTCGTGTCAGCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGGTGAcgt                              <  2:187711/250‑1 (MQ=255)                                                                                                                                                                                                                        tAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTACAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGAcgtcgt                           >  2:302085/1‑251 (MQ=255)                                                                                                                                                                                                                                        gtgcggtATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTACAAGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGCAGTTGACGTCGTCACGCTCGGTGCAAcc           >  1:160981/1‑251 (MQ=255)                                                                                                                                                                                                                                            ggtATCGACTTTCGGGTTATAGCGCACCACATCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTGCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTACAGGCGCGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCg       >  1:240174/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cGACTTTCGGGTTATAGCGCACCACATAAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATATTCACGTCGCTGTACTCCAAGCGCGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGCCGTCGTCACGCTCGGTGCACCCTTCGTcccg  >  2:361003/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAG  >  NC_000913/4380223‑4380713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑