Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	100.0%	142.5 / NA	43	A272T (GCG→ACG)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

GGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCG  >  NC_000913/4373174‑4373659                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ggCTGGGTGACAATCGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGGTAGCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAATGATGAGAGTATTTCTGCTGTGGGGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGcgtgcg                                                                                                                                                                                                                                             <  1:29635/251‑1 (MQ=255)      ggTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGc                                                                                                                                                                                                                                        <  1:226776/251‑1 (MQ=255)           cAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTg                                                                                                                                                                                                                                    >  1:33383/1‑250 (MQ=255)                   taatggcatGATGTGCTTCGCTTATCAGGCAAACATATCGCATTGCAACTGATTGACTGCTTGAGAATTTGTATGACGGATAAGGCGATTACGCCGCATCCGGCATGACAATCCATCACGTTCTCAGTCATTTCAGGCTGGATGACTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGAGCAGCATTGCTGAAGGATTGCACACGTAAGTGATGCAGCGTGCATAAGCATTGATCCTGGGt                                                                                                                                                                                                                           >  2:13850/9‑251 (MQ=255)                             aTGCGCTTCGCGTATCAGGCCTACATAAGGCATGGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACTCGTAAGTGATGCAGCGTGCGTAAGCATGGATCCTGGGTTAGCGGTTgg                                                                                                                                                                                                                 <  2:33383/251‑1 (MQ=255)                              tGCGCTTCGCTTATCAGCCCTACTTAAGGCATTGCAACTGATTGTATGCTTGAGAATTTGTAGACCGGCTAAGGCGTTTACGCCGCATCCGACCTGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCCCCAGCCTCGCATTAGCAAAGATGAGAATATTTCGCCTGTGGTTCATTTTTGCTGGTGGATTGAACTAGTTAGGGATGCATCGTACGTAAGCATTGATCCAGCGTTATCGGTTgtg                                                                                                                                                                                                                >  2:331694/1‑249 (MQ=255)                               gcgcTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTggg                                                                                                                                                                                                                <  1:13829/250‑1 (MQ=255)                                   ttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTCGTCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                                                                                                            >  2:294343/1‑250 (MQ=255)                                          aTCAGGCATACATAAGGCATTGCAACTGATGGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgcc                                                                                                                                                                                                     <  1:119460/250‑1 (MQ=255)                                                       aaGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAAc                                                                                                                                                                                       >  1:293951/1‑251 (MQ=255)                                                         ggCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGAGCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGAGTGAACACGTAAGGGAGGCAGCGTGCGTAAGCAGTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAt                                                                                                                                                                                     <  1:181697/251‑1 (MQ=255)                                                                     gATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACa                                                                                                                                                                           <  1:294343/249‑1 (MQ=255)                                                                         gAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACGCGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGcac                                                                                                                                                                     <  1:232127/251‑1 (MQ=255)                                                                                   gaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGt                                                                                                                                                           <  2:265618/251‑1 (MQ=255)                                                                                     aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGttt                                                                                                                                                         <  1:348172/251‑1 (MQ=255)                                                                                               cGGATAAGTCGTTTACGCCGCATCCGGCATGACCAACGATCACTTTATCAGTCATTTCTGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGCCATGGCACACCCGCGAGTTTTTTCAGTCGt                                                                                                                                               <  2:314930/251‑1 (MQ=255)                                                                                                 gacaaGGCGTTTACGCAGCATCAGTCATGAACAACGCTAACGTTATCAGTAATTTCAGGCTGGAAGGCTCCAGCCACGCATTAGCAAATATGCGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGCTGCAGCGTGCGTAAGCATTGATCATGGTTTAGCGGTTGGGCGTTATTCTCCGCTACGGTGCGAACATAGACATGACCTGGCACACCCGCGAGTTTTTTCAGTCGTgg                                                                                                                                             >  2:298634/4‑251 (MQ=255)                                                                                                    aaGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCt                                                                                                                                          >  1:139157/1‑251 (MQ=255)                                                                                                           ttACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATc                                                                                                                                    >  2:148615/1‑250 (MQ=255)                                                                                                             aCGCCGCATCCGGCATGCCCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTAc                                                                                                                                                                                                >  1:286704/1‑188 (MQ=255)                                                                                                             aCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTAc                                                                                                                                                                                                <  2:286704/188‑1 (MQ=255)                                                                                                                             gACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATc                                                                                                                 <  1:380380/251‑1 (MQ=255)                                                                                                                                cAACGATCACGTTCTCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTAGTGGGCTGAAATCAAGAATTCGGCGAGTGATCGCt                                                                                                              >  1:313460/1‑251 (MQ=255)                                                                                                                                   cGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCt                                                                                                           >  1:249309/1‑251 (MQ=255)                                                                                                                                     aTCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAt                                                                                                         >  2:311888/1‑251 (MQ=255)                                                                                                                                       cACGTTATTAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAtt                                                                                                        <  2:301301/250‑1 (MQ=255)                                                                                                                                                                aTGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGt                                                                              <  2:249309/251‑1 (MQ=255)                                                                                                                                                                        aGCCTCGCATTAGCAAATATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAATTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcacca                                                                      <  1:228816/251‑1 (MQ=255)                                                                                                                                                                        aGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcacca                                                                      <  1:307582/251‑1 (MQ=255)                                                                                                                                                                           cgcGCATGAGCAAAGATGAGATTATTTCGCCTGAGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGTTCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGt                                                                                                                                                  >  2:54868/3‑172 (MQ=255)                                                                                                                                                                           cTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGAGGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGt                                                                                                                                                  <  1:54868/172‑1 (MQ=255)                                                                                                                                                                                   aGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGAACCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTa                                                           <  1:262610/251‑1 (MQ=255)                                                                                                                                                                                   aGCAAAGATGAGAGTATATCTCCCGTGTTGCAGTGTGTCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTGAGCGGTTGGGCGGGATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCGGAAATCAAGCATTCGGCGAGTGATCGCTCCTATTTCTTTACCCTGCGTAGCATCACGCGTTTTCATCACTGCGCCGCTa                                                           <  2:54546/251‑1 (MQ=255)                                                                                                                                                                                            gagaTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTc                                                   <  2:220115/250‑1 (MQ=255)                                                                                                                                                                                                 tattTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGcc                                             >  1:84201/1‑251 (MQ=255)                                                                                                                                                                                                 tatctCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGcc                                             >  1:84213/5‑251 (MQ=255)                                                                                                                                                                                                      cGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACttt                                        <  1:5058/251‑1 (MQ=255)                                                                                                                                                                                                        ccTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGCGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTc                                       >  1:166457/1‑250 (MQ=255)                                                                                                                                                                                                              gtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTc                                       >  1:353365/1‑244 (MQ=255)                                                                                                                                                                                                              gtgCAGTGTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTAGTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTc                                       <  2:353365/244‑1 (MQ=255)                                                                                                                                                                                                                        gCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTaa                       <  1:148615/250‑1 (MQ=255)                                                                                                                                                                                                                                      cacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAGCATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTACAGCACCGCCAGATa         >  2:378662/1‑250 (MQ=255)                                                                                                                                                                                                                                         gTAAGTGATGCAGCGTGCGTAAGCATTGATACTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTACATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTCAAGCACCGCCAGATCATgg     >  2:355703/1‑251 (MQ=255)                                                                                                                                                                                                                                            agggATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCg  >  2:266249/4‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCG  >  NC_000913/4373174‑4373659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑