Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,401	C→T	A126V (GCT→GTT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,401	0	C	T	98.4%	208.9 / NA	62	A126V (GCT→GTT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (29/32);  minor base G (0/1);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGCTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAG  >  NC_000913/4371159‑4371645                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     tGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGCCAAAGCGGTTACCGTTGCAGTTg                                                                                                                                                                                                                                              >  1:17614/1‑251 (MQ=255)   cACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTgaa                                                                                                                                                                                                                                            <  2:14626/251‑1 (MQ=255)    aCCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTga                                                                                                                                                                                                                                             >  2:298325/1‑249 (MQ=255)         ccATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTg                                                                                                                                                                                                                                      >  1:61730/1‑251 (MQ=255)         ccATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTg                                                                                                                                                                                                                                      <  1:326559/251‑1 (MQ=255)               ccAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAGACGACGCTGCAGGCGACGGTACCATCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAGAGCTGTTGCTGGGGGCATGAACCCGATGGACCTGAAACGGGGTATCGACAGAGCGGTTACCGTTGCAGTTGAAGAACTGAAAggg                                                                                                                                                                                                                                >  1:389448/1‑249 (MQ=255)               ccAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGAGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAgcg                                                                                                                                                                                                                                >  2:370245/1‑251 (MQ=255)                  aaGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTTAAAGCGCTg                                                                                                                                                                                                                             <  2:78027/251‑1 (MQ=255)                                   gCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGACAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCACTg                                                                                                                                                                                                             >  2:56791/1‑250 (MQ=255)                                    cTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGAc                                                                                                                                                                                                           >  1:125650/1‑251 (MQ=255)                                    cTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGAc                                                                                                                                                                                                           >  1:302464/1‑251 (MQ=255)                                       gTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGActct                                                                                                                                                                                                        <  1:46791/251‑1 (MQ=255)                                       gTGAAATCGAACGGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGActct                                                                                                                                                                                                        <  2:383018/251‑1 (MQ=255)                                            aTCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTAACACCACTGCAACCGTACTGGCTCCGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGGCAAAGCGGTTACCGTTGCAGTTGCAGAACTGAAAGCGCTGTCCGTAcc                                                                                                                                                                                                                     >  2:94278/1‑233 (MQ=255)                                            aTCGAACTGGAAGAAAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTAcc                                                                                                                                                                                                                     <  1:94278/233‑1 (MQ=255)                                                       aGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCGGACTCTAAAGCGATTGCTCAgg                                                                                                                                                                                        <  1:127879/251‑1 (MQ=255)                                                          cAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACTCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGATCTGAAAGCGCTGTCCGTACCATGCGCTGACTCTAAAGCGATTGCTCAGGTTg                                                                                                                                                                                     <  2:28081/251‑1 (MQ=255)                                                           aaGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCTCCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTgg                                                                                                                                                                                    <  1:404095/251‑1 (MQ=255)                                                                      tatGGGTGCGCAGCTGGTGCAAGAAGTTGACTCTAAAGCACACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATAATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGAGATTGCTCAGGTTGGTACGATCTCCg                                                                                                                                                                         >  2:140960/1‑251 (MQ=255)                                                                         gggTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGATCTGAAAGCGCTGTCCGTGCCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCt                                                                                                                                                                       <  1:51149/250‑1 (MQ=255)                                                                               gcAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCGGCGGGCGACGGTAACACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCGGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCGGACTCTAAAGCGAGTGCGCAGGTTGGTACCATCGCCGCTAACTCcg                                                                                                                                                                <  2:91058/251‑1 (MQ=255)                                                                                 aGATGGTGAAAGAAGTTGCCTCTACAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCcgac                                                                                                                                                              >  2:198132/1‑251 (MQ=255)                                                                                       tGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAAcc                                                                                                                                                        >  2:385439/1‑251 (MQ=255)                                                                                         aaaGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGt                                                                                                                                                      <  1:86855/251‑1 (MQ=255)                                                                                          aagaagTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGt                                                                                                                                                      >  2:189515/1‑250 (MQ=255)                                                                                              atttGCCTCTAATGCAAGCGACGCTGCATGCGACGGTACCTCCACTGCTACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGATCCCGATGGACCTTAAACGTGGTATCGACTAAGCGGTTACCGTTGCAGTTGAATAACTGAAAGCGCTGTCCGTTCCATGCTCTGACTCTAAAGCGATTGCTCATGTTGTTACTATcgccgcggaatccttcgagaccttaggta                                                                                                                                                 <  2:264764/249‑24 (MQ=255)                                                                                                   ccTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGAACCATGCTCTGACTCTAAAGCGATTGCTCAGGATGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTg                                                                                                                                            >  1:174336/1‑251 (MQ=255)                                                                                                   ccTCTAAAGCAAACGACACTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCGCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTg                                                                                                                                            <  2:307461/251‑1 (MQ=255)                                                                                                            cAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAGCCGTCGGTAAACTGATccatga                                                                                                                                    >  2:138962/1‑245 (MQ=255)                                                                                                            cAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGaa                                                                                                                                   <  1:385788/251‑1 (MQ=255)                                                                                                            cAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGATCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGCAGAACTGCAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGa                                                                                                                                    >  2:31310/1‑250 (MQ=255)                                                                                                                cgacgCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTAGCAGCACTGAAGGTCTGAAAGCTGTTGCGGCGGGCATGAACCCGATGGACCTGAAACGTGGGATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCAGGCTCTGACTCTAAAGCGAGTGCTCAGGTTGGTACCAGCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGa                                                                                                                               <  2:204798/251‑1 (MQ=255)                                                                                                                 gacgCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGAt                                                                                                                              >  2:160551/1‑251 (MQ=255)                                                                                                                   cgCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATgg                                                                                                                            <  1:312871/251‑1 (MQ=255)                                                                                                                               cGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTa                                                                                                                <  1:91595/251‑1 (MQ=255)                                                                                                                                    ccaccaCTGCAACCGTACTGGCTCAGGCTATCATCACTGAATGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGCTCGCTGAAGCGATGTACAAAGTCGGTAaagaa                                                                                                           <  2:293192/251‑1 (MQ=255)                                                                                                                                       ccacTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTCCCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGc                                                                                                        >  1:347675/1‑251 (MQ=255)                                                                                                                                       ccacTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAggg                                                                                                        >  1:51518/1‑250 (MQ=255)                                                                                                                                            gCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTa                                                                                                    <  2:253896/250‑1 (MQ=255)                                                                                                                                              aaCCGTACTGGATCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGCCAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGATGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATc                                                                                                  >  2:351811/1‑250 (MQ=255)                                                                                                                                                  gTACTGGCTCAGGCTATCATCACTGAAGGGCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGt                                                                                             >  2:97617/1‑251 (MQ=255)                                                                                                                                                      tGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGGTGGTACCATCTCCGCTAACTACGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGa                                                                                          >  2:1405/1‑250 (MQ=255)                                                                                                                                                        gCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATTGTCCTGAAACGTGGTATCGACAAAGCGGTTACCTGTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTTCTCAGGTTGGTACTATCTCCGCTAACTCCTACGAAACCTTAGGTAAACTGATCGCTGAAGCGATGGACAAAGACGGTAAATAATTCGTTATCACCGGTGAAGa                                                                                       <  1:319089/251‑1 (MQ=255)                                                                                                                                                                          gAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGa                                                                     <  1:342121/251‑1 (MQ=255)                                                                                                                                                                           aaGGTCTGAAAGCTGTTGCTGCGGGCATGAACCTGCTGGACCTGAACCGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGGACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGAc                                                                    >  2:65303/1‑251 (MQ=255)                                                                                                                                                                                  gAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTgg                                                             >  2:190282/1‑251 (MQ=255)                                                                                                                                                                                     aGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCTGTCTGCAGGACTAACTGGACg                                                          <  1:326033/251‑1 (MQ=255)                                                                                                                                                                                      gCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGt                                                         <  1:190282/251‑1 (MQ=255)                                                                                                                                                                                           tgctgcGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGtt                                                     <  1:328643/250‑1 (MQ=255)                                                                                                                                                                                            gctgctGGCATGATCCCGATGGGCTTGAAACGTGGTATCGACAAAGCGGTGACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTTCCATGCTCTGACTCTAAAGCGATTGCTCAGGTTTGTACCATCTCCGCGATCTCCTACGAAACCGTAGGTAAACTTACCGCTGAAGGGATGTACAGAGTCGGTAAAGAAGTCGTGCTCGCCGGTGAAGACTGTACCGGTCTGTGGGACGAGATGGACGTGGTTg                                                    <  2:401028/250‑1 (MQ=255)                                                                                                                                                                                                 gggCATGAACCCGATGGACCTGAAACGTGGCATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCACTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGt                                               >  1:114591/1‑250 (MQ=255)                                                                                                                                                                                                   gCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGACGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGCAGACGGTACCGGTCTGCAGGACGAACTGGACGTGTTTGAAGTTATg                                            >  2:144022/1‑251 (MQ=255)                                                                                                                                                                                                       gAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAg                                         <  1:198132/250‑1 (MQ=255)                                                                                                                                                                                                       gAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAg                                         <  1:351811/250‑1 (MQ=255)                                                                                                                                                                                                           ccGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGCAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCg                                     >  1:379240/1‑250 (MQ=255)                                                                                                                                                                                                            cGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCg                                                                                                                                                                         >  1:323270/1‑117 (MQ=255)                                                                                                                                                                                                            cGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAGCTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGAc                                   >  1:70276/1‑251 (MQ=255)                                                                                                                                                                                                            cGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGGTGGTACCATCTCCg                                                                                                                                                                         <  2:323270/117‑1 (MQ=255)                                                                                                                                                                                                                         aCGTGGTATCGACAAAGCGGGTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGt                      <  2:51518/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGtc                     <  1:370245/251‑1 (MQ=255)                                                                                                                                                                                                                           gTGGTATCGACAAAGCGGTTACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGtct                    <  1:87896/251‑1 (MQ=255)                                                                                                                                                                                                                                              tACCGTTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAg  <  1:226793/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     TGCACCGACCATCACCAAAGATGGTGTTTCCGTTGCTCGTGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGTGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCAAACGACGCTGCAGGCGACGGTACCACCACTGCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATGGACCTGAAACGTGGTATCGACAAAGCGGTTACCGCTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCATGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAG  >  NC_000913/4371159‑4371645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑