Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,668	C→T	A36T (GCC→ACC)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,668	0	C	T	100.0%	63.9 / NA	22	A36T (GCC→ACC)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

CAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTAT  >  NC_000913/4366425‑4366913                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cAGCGCTAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGAGCTGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAAcc                                                                                                                                                                                                                                                >  1:61622/1‑251 (MQ=255)                            agaTGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAAcc                                                                                                                                                                                                                      >  1:386935/1‑250 (MQ=255)                                                  gATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGAGGTAAGAACCAGCACACCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTgg                                                                                                                                                                                               >  1:339103/1‑251 (MQ=255)                                                        cAGGAAATAGGTAACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGCTAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAAAGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGcca                                                                                                                                                                                          >  1:279493/1‑249 (MQ=255)                                                                                                                     ttttCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACCACTAGCATATTAGCCTTCCTTGtttt                                                                                                                            >  2:291745/1‑251 (MQ=255)                                                                                                                                   acccaaatagccccccccGCCAGCAACCTCCTTGGAAAAAATAGCGGCAATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGata                                                                                                              <  1:172952/235‑1 (MQ=255)                                                                                                                                              ccAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGCTA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAAATAGCATATTAGCATTCCTTGTTTTTTAACAAGTTGATATTAGATTGttt                                                                                                   >  2:193042/1‑250 (MQ=255)                                                                                                                                                  aCCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCACGCAGATCGCCCGCTAAACTATGATGCGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTAtttt                                                                                               >  2:110800/1‑251 (MQ=255)                                                                                                                                                    cGCCACCAACCTGCAGGGAATAAATGGCGGCG‑TATACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTGAACAAGTTGATATTAGATTGTTATTTTTa                                                                                             <  1:1026/251‑1 (MQ=255)                                                                                                                                                                   aggcaaaaaataggggttaaatccGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTcaca                                                                               <  1:285252/229‑1 (MQ=255)                                                                                                                                                                    ggcgaaaagaggggtaaTA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAg                                                                             <  2:298093/235‑1 (MQ=255)                                                                                                                                                                     acaaaaaggggtgcacaa‑aCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGAGCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGa                                                                            <  2:356100/234‑1 (MQ=255)                                                                                                                                                                            tAGCGTCGATA‑CCCGCCCTGATAATGGAGATGACATCGAACGGGCTGTTACAGTGCTTAACGCCAATAGCGGTAAGAACAAGCACACCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCAAAGAAGATCGCCAGCCAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTCACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGaaaaaatg                                                                     >  2:198568/1‑249 (MQ=255)                                                                                                                                                                            gggcggcgATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAgg                                                                     <  1:306444/249‑1 (MQ=255)                                                                                                                                                                                             cATGATAATGGAGATTACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTg                                                     <  2:346231/251‑1 (MQ=255)                                                                                                                                                                                              aTGATAATGGAGAAGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTAGTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGGTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc                                                    <  2:365782/251‑1 (MQ=255)                                                                                                                                                                                                    aTGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                              >  2:295049/1‑251 (MQ=255)                                                                                                                                                                                                      ggAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTa                                            <  1:3446/251‑1 (MQ=255)                                                                                                                                                                                                                tCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtactac                                  >  1:84170/1‑251 (MQ=255)                                                                                                                                                                                                                     cGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGt                             >  2:82765/1‑251 (MQ=255)                                                                                                                                                                                                                                      tAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCt             <  1:131919/250‑1 (MQ=255)                                                                                                                                                                                                                                                aGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGtat  <  1:283004/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA‑ACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTAT  >  NC_000913/4366425‑4366913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑