Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,590	C→T	V25I (GTC→ATC)	dcuS ←	sensor histidine kinase DcuS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,590	0	C	T	97.2%	102.1 / ‑5.7	36	V25I (GTC→ATC)	dcuS	sensor histidine kinase DcuS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/20);  minor base G (0/1);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

GACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGG  >  NC_000913/4351347‑4351836                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         gACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAg                                                                                                                                                                                                                                                   <  2:178808/251‑1 (MQ=255)       gcttaccGGTAACGTCAATAAACATCAGATCGTTGCCTTTGCGTACGGCTTCCTCGATTGCCTGGATGCCACTCTCCTGCGGTTTTTTCTTCAAGCCCTGACGGAGTTCCGGCGAGTCGGCGAGGGTACGCGCCCCTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGGAGTAAATCAGATGCCCCACCAATAGCACCGAGAACAGTACCGCACTGAGTATTAAGATCACt                                                                                                                                                                                                                                             <  2:177964/245‑1 (MQ=255)           atCGGTAACGACAATAACCAGCAGACCGTTGCGTTTTCTTACGGTTGCCTCGATGGCCGGGATGCCACTCTCCGGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGATAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtgtg                                                                                                                                                                                                                                          <  2:222818/250‑1 (MQ=255)             cGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGt                                                                                                                                                                                                                                        >  1:320506/1‑250 (MQ=255)                   cGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAAt                                                                                                                                                                                                                                >  1:96358/1‑251 (MQ=255)                            aCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTAATCgg                                                                                                                                                                                                                        >  2:280467/1‑250 (MQ=255)                                     cGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTCCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGt                                                                                                                                                                                                              <  2:319044/251‑1 (MQ=255)                                      gTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGt                                                                                                                                                                                                              >  1:117428/1‑250 (MQ=255)                                            tttgcgtACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCACTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTATTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACAGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGc                                                                                                                                                                                                       >  1:29005/1‑251 (MQ=255)                                                 gatcGGCTTGCACGATGGCGTGGAGTCCACTCGGCTGCGGTTTGTTCTGCAAGCCCTGACGGTTTTCCGGCGAGTCGGCGAGGGTACGAGCCACTGCCAGTGCCTTGTTGGCTAGATCATCTCGTGTCAGATCCCTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCAACGAGAACAGTACCGCACGGATCATTAAGATCACTGTG‑TAGATCAATTGCAGCGGACGTGTGTGTAACATGCGGTa                                                                                                                                                                                                   <  2:82160/247‑1 (MQ=255)                                                   aCGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAgg                                                                                                                                                                                                 <  2:117428/250‑1 (MQ=255)                                                         tCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCaatg                                                                                                                                                                                           <  1:379970/250‑1 (MQ=255)                                                              gATGGCCTGGATGCCACTCTCCTGCGGTTTTTTATGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGCGAGTACGCGCCACAGCCAGTAACCTGTTGGCTAGCCAATCTAGCGTCACATCACTGATTTGCGAGAAGTAAATCAGATGCCCCGCCAATCGCACCGAGAAAAGTCCCGCACTGCTCATTCAGATAACTGTGGAA‑CTCAATTTCCTCGGCAGTTTGTGTAACATGCGGTAGGGCAATGAATGtc                                                                                                                                                                                     >  1:394018/1‑251 (MQ=255)                                                                      ggATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAAGTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGc                                                                                                                                                                             >  1:307923/1‑251 (MQ=255)                                                                       gATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCt                                                                                                                                                                            <  1:60954/251‑1 (MQ=255)                                                                          tccACTCTCCTGCCGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGACTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCACGCGTCCTATCAATGATTAGCGAGACGAAAATCAGATGCACCCCCACTAGCACCGAGAACAATACCGCACTGATCATTAAGATCACTGTAGTA‑CTCAATTTACTCGGCCGTATGCGAAACATGCGCAAGGGCAATGAATGTCTCATCAgtttac                                                                                                                                                                         >  2:215266/2‑246 (MQ=255)                                                                           ccACTCTCCTGCGGTTTTTTCTGCAAGCCATGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCt                                                                                                                                                                        >  2:241064/1‑251 (MQ=255)                                                                              ctctCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgt                                                                                                                                                                     >  2:286341/1‑251 (MQ=255)                                                                                    tGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGGA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGAc                                                                                                                                                                >  1:77136/1‑250 (MQ=255)                                                                                       ggTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATAGCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCATCGTCCTTGTGTGACAAAt                                                                                                                                                            <  2:77136/251‑1 (MQ=255)                                                                                           ttttCTGCAATCCCTTACGGATTTCCTGCGAGCCGGCGAGTGTACGCGCCCCTGCCAGTGTCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCTCCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCAGCAGCTTCCTTGTGTGACAAATTTc                                                                                                                                                         <  2:12515/250‑1 (MQ=255)                                                                                                                 ttCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGc                                                                                                                                  >  1:402617/1‑251 (MQ=255)                                                                                                                                           ccACTGCCAGTGTCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGAATTATCTCTGATGAGGCGTGTAATTCAAAGGGAGTAAGAATGa                                                                                                        <  1:257953/251‑1 (MQ=255)                                                                                                                                                      gCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCtatata                                                                                             <  1:286341/251‑1 (MQ=255)                                                                                                                                                           gttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAgggg                                                                                         <  1:218830/250‑1 (MQ=255)                                                                                                                                                                  aGCCCATCTCGTGTCATATCACTGATGTGCGAGAAGTAAATCAGATGCACCACCAAGAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGGGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGAct                                                                                 <  1:251681/251‑1 (MQ=255)                                                                                                                                                                         atcgtgTCATATCACTGATTTGCGAGAAGTAAATCAGATGCCCCACCAATAGCACCTAGAACAGTACCGCACTTATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATTTCTCATCAGCTTCCTTTTGTGACAAATTTCTTAAGCAGTATCTATGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGAGTGGCTATATAGTGGAAGCGATTCTGGCa                                                                           <  2:42367/245‑1 (MQ=255)                                                                                                                                                                                 atatCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACt                                                                  <  1:320676/251‑1 (MQ=255)                                                                                                                                                                                          aTTTGCGAGAAGTAAATCAGATGCACCACCAAGAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCt                                                        <  2:248175/251‑1 (MQ=255)                                                                                                                                                                                                         aTCAGATGCACAACCAATAGCACCGAGAACTGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCTGTATGAAGATTCCgg                                         <  1:291305/251‑1 (MQ=255)                                                                                                                                                                                                                             cACCGAGAACAGTTCCGCACTGATCATTAAGATCACTTTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCTGTATGAAGATTCCGGGGCTATGCTtata                            <  1:218288/244‑1 (MQ=255)                                                                                                                                                                                                                             cACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTTTAAGGGTGGAAAAATGATTGGCTATATAGGGGAAGAGACTCTGGAGACGGAAACAGCACA‑TGCTGTATGAAGGTTCCGGGGCGATGCTtataaaatcgg                     >  2:218288/1‑244 (MQ=255)                                                                                                                                                                                                                                   gaACAGTACCGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGGAATTCCAAGGGGGGAAGAATGCTTTGCTATATAGGGGCAGAGACTCTGGCAACAGACAATGG‑CAGGGATGTATGAAGATTCCGGGGGTATGGTTATAGCGATAATAATAc               >  1:372495/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccGCACTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGTAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATgaga       <  1:241064/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCAGCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                    <  1:185471/155‑1 (MQ=255)                                                                                                                                                                                                                                                cTGATCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGGAATTCAAAGGGGGTAAGAATGATTGGTGCTATAGGGGGAAAGACTCTGGGAACGGAAACTGC‑CAGTGGTGTATGAAGATTCCGGGGGTATGCTTATAGCGGTAATTATACtggtgggaggggg  >  1:328413/1‑240 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         GACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTA‑CTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGC‑CAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGG  >  NC_000913/4351347‑4351836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑