Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,349,259	C→T	intergenic (‑515/+56)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,349,259	0	C	T	100.0%	102.5 / NA	31	intergenic (‑515/+56)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

ATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGAA  >  NC_000913/4349031‑4349498                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aTTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACgg                                                                                                                                                                                                                            <  1:289148/250‑1 (MQ=255) aTTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGt                                                                                                                                                                                                                           <  2:308003/251‑1 (MQ=255) aTTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGt                                                                                                                                                                                                                           <  1:73425/251‑1 (MQ=255)     tttGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGtaat                                                                                                                                                                                                                        <  2:151645/250‑1 (MQ=255)     tttGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGtaat                                                                                                                                                                                                                        >  1:242804/1‑250 (MQ=255)            tcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAattatt                                                                                                                                                                                                                 >  1:115913/1‑250 (MQ=255)                     aTGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttacttac                                                                                                                                                                                                           >  2:103099/1‑247 (MQ=255)                               gTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGAt                                                                                                                                                                                             <  1:298511/251‑1 (MQ=255)                                  tataGATTAATCTGCTCTACCCATTTCTGGGTAAAAATACACATAATGCGCGTGACATATTAGTTACTTAACTTTTGTTAGCGTTTTGAAATTAAAAACAACGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCg                                                                                                                                                                                          >  2:151345/1‑251 (MQ=255)                                             cTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACATGAAGAGATATTAATTTTTAGAGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGAATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAat                                                                                                                                                                               >  2:345459/1‑251 (MQ=255)                                                                  aaaaaTACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCagtagtgagtag                                                                                                                                                           <  2:107141/250‑1 (MQ=255)                                                                          acaTAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGcc                                                                                                                                                  >  1:178283/1‑251 (MQ=255)                                                                            aTAATGCGGGCGACATAATAGTTCATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGAGTTCTGCCTg                                                                                                                                                >  2:146583/1‑251 (MQ=255)                                                                                  cGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTACTTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATacg                                                                                                                                          >  1:153397/1‑251 (MQ=255)                                                                                     gTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACATGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACATATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATacggt                                                                                                                                        >  1:218990/1‑250 (MQ=255)                                                                                                gTTAATTAACGTTGGTGAGCGTAGTGAAATAAAAAACACCGTGCAACTGAAGAGAAATTAATTTGTAGCGATGATGGAGGGATAATTATATTTGATCTGGCAAAAGTGTTACTGATAAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAAGTATTACGTACAGAGAACCAGCGATCCGTGATTAGCAATATTGTTTCAGGAGTGAGTAGTGTGCGGCCTGAAAAGGATACCGGTAAACTg                                                                                                                            <  2:236645/251‑1 (MQ=255)                                                                                                 ttaattaaCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGAAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTgg                                                                                                                           >  1:337588/1‑251 (MQ=255)                                                                                                   aattaaCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGAc                                                                                                                         >  1:115848/1‑251 (MQ=255)                                                                                                   aattaaCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTGTAGCTATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTTATGATGAAGGATGTAACTTGTGTTAGGGGTATTTTGCATTACGTTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTGCAGTAGTGAGTAGTGGTCTGCCTGAATACGGTAACGGTAAACTGGAc                                                                                                                         <  2:135083/251‑1 (MQ=255)                                                                                                          ttttGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTCATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGAAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt                                                                                                                  >  1:27182/1‑251 (MQ=255)                                                                                                                                                gAAGAGATATTAATTTTTAGCGATGATGGCGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGt                                                                            >  2:199347/1‑251 (MQ=255)                                                                                                                                                              ttttAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTAGTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTTACCAGCCAGAt                                                               <  2:242804/250‑1 (MQ=255)                                                                                                                                                                                           ttGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAAt                                 >  1:276349/1‑251 (MQ=255)                                                                                                                                                                                            tGATCTGGCACAATGTTTACTGATTAAGGATGTAACTTGTGTCAGGGGTAATTTGCTTTACGGTAATAATTATTACTTACAGATAACCAGCGCCCCGATATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGTTATTTACTGCAGGAACCACGCGAAAt                                 <  2:212698/250‑1 (MQ=255)                                                                                                                                                                                             gATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGt                               >  1:231225/1‑251 (MQ=255)                                                                                                                                                                                                            ttACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATTCGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACGCGCGAAATGTTAACCTCGTTGGCt                 <  2:337588/250‑1 (MQ=255)                                                                                                                                                                                                             tACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCt                 >  1:55143/1‑249 (MQ=255)                                                                                                                                                                                                                  atgaAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGAGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTCCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTAAGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCtcgt          >  1:202582/1‑251 (MQ=255)                                                                                                                                                                                                                   tgaAGGATGTAACTTGTGTCCGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGACACACGCGAAATGGTAAACTCGGTGGCTAGTtcgtc         >  2:346784/1‑251 (MQ=255)                                                                                                                                                                                                                    gaAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCtcgtc         <  2:55143/250‑1 (MQ=255)                                                                                                                                                                                                                           gTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGaa  <  2:71633/250‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGAA  >  NC_000913/4349031‑4349498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑