Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,825	C→T	N305N (AAC→AAT)	melA →	alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,825	0	C	T	97.8%	148.7 / ‑4.9	45	N305N (AAC→AAT)	melA	alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/22);  minor base G (1/0);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTG  >  NC_000913/4342584‑4343065                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  aaCTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGAGTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑Taaa                                                                                                                                                                                                                                         <  1:159859/251‑1 (MQ=255)                aTGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGGGAATTGGCA‑TAAAGAGCTGGATGAGtat                                                                                                                                                                                                                          <  2:153931/251‑1 (MQ=255)                           caggcaCCGAAGCCGAATATTCATGGCAATACGCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTc                                                                                                                                                                                                               <  1:303488/251‑1 (MQ=255)                              gcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCg                                                                                                                                                                                                            <  1:369022/251‑1 (MQ=255)                                aCCGAAGCCGACTATTCATGGCAATACTCGCTGCAAGAATATTGTGCGCTACGCAATGTTCAAAAACATGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGACAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAACCGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGCGTATAAAACAGCCTCCCgg                                                                                                                                                                                                           >  2:355011/1‑250 (MQ=255)                                           atatTCATGGCAATACTCGATGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGATGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTaa                                                                                                                                                                                                >  2:43623/1‑250 (MQ=255)                                            tatTCATGGCAATACACGCTGCCCGAATATTGTGCGCTACGAACTGTTCAAAAAGATGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAc                                                                                                                                                                                              >  2:195003/1‑251 (MQ=255)                                                cATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTc                                                                                                                                                                                          >  2:88076/1‑251 (MQ=255)                                                cATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTCGGCTATTTCGTAACGGCATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAACGAGCTGGAGGCGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTc                                                                                                                                                                                          >  1:62135/1‑251 (MQ=255)                                                     cAATACTCGATGCCAGAATATTGTGCGCTCCGAAATGTTAAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGATCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACggg                                                                                                                                                                                     >  2:140745/1‑251 (MQ=255)                                                        tACTCGCTGCCAGAATATTGTGCGCTACGCAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGCTTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACACGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAat                                                                                                                                                                                  >  2:391145/1‑251 (MQ=255)                                                          cTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAatat                                                                                                                                                                                >  2:240018/1‑251 (MQ=255)                                                                  cAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGcac                                                                                                                                                                        >  1:246742/1‑251 (MQ=255)                                                                  cAGAATATTGTGCGCTACGAAATGTTAAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACTTTTTGCTGAGTATACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTTAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTTGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGACACGGGAATATGCCAGcac                                                                                                                                                                        <  2:171363/251‑1 (MQ=255)                                                                        atTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCAt                                                                                                                                                                  <  2:112577/251‑1 (MQ=255)                                                                                    gAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACTCCGTGGTTTATTAAGCCGGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTg                                                                                                                                                      >  1:324192/1‑251 (MQ=255)                                                                                         gTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATGAAGCCAGGGCGTGAGGATTTGAGTGAGCGTGATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTTGCA‑GAAAGAGCTGGAGGATTATAAAAAATCCGCCCGGAGTGATATTAAACCGTCACGGGAATATGCCAGCACAATTAGGAAGGCTATCTGGACt                                                                                                                                                  <  2:156868/250‑1 (MQ=255)                                                                                                     gggCTATTTCTTAACGGAATCAGCAGAACCTTTTGCTGAGTACACCCCGTGTTTGATTAAGCTATGTCGTGTGGATTTGATTGAGCGTTCTAAAGTGCCGCTGGATGAGTACCCGAAACGCTGCGCCGAGCAGCTGGCTAATTTGCA‑TAAAGATCTGGAGGTGTATAAAAAATCCTCCCGGATGGATATTAAACCGGCACGGCAATATGCCAGCACAATCATGAACGCTATCTGGTCTGGCGAGCCGat                                                                                                                                       <  2:211409/249‑2 (MQ=255)                                                                                                               gTCACGGAATCGTCAGCAAGTTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGCTTGATATTAAACCGTCACGGGAATATGCCAGCACCATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACgg                                                                                                                           >  1:348098/1‑251 (MQ=255)                                                                                                                         cGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCg                                                                                                                  <  1:21834/250‑1 (MQ=255)                                                                                                                            cAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAAc                                                                                                              >  2:209084/1‑251 (MQ=255)                                                                                                                              gAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACg                                                                                                             >  2:305916/1‑250 (MQ=255)                                                                                                                                 cATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGTACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATgg                                                                                                         <  2:39006/251‑1 (MQ=255)                                                                                                                                  aTTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGAAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGAATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTCCGGCAACGTCCGTAACGATGGt                                                                                                        >  2:304312/1‑251 (MQ=255)                                                                                                                                          gAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgattg                                                                                                 <  1:391145/250‑1 (MQ=255)                                                                                                                                              tcgcacCGTGGTTTTGTAAGCCAGGTCGTGAGGATGTGATTGAGCGTTATAAAGTACCGCGGGATGAGTACCCGAAACGCTGCGTCGAGCAGGTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGGATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGTGAATATGCCAGCACAATCATGAACGCTCTCTGGACGGGCGAGCCGAGTGGGATTTACGGCATGGTTCTTAACGATGGTTTGATTGATaa                                                                                             <  2:357629/247‑1 (MQ=255)                                                                                                                                                      tGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCcac                                                                                     >  1:105944/1‑250 (MQ=255)                                                                                                                                                       ggTTGATTCAGCCTGGTCGTGAGGTTTGGATTGAGCGTAATAATGTACCGCTGGATGAGGACCAGAAACGCTGCGTCGAGCAGCTGGCAAATTGCCT‑TAAAGAGCTGGAGGAGTAGAAAAAAGCCTCCCGGATTGATATTAAACCGTCCCGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCACCGTCCGTACCGATGTTTTGATTGATAACCTGCcaca                                                                                    <  2:81343/250‑1 (MQ=255)                                                                                                                                                            attaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGAt                                                                               <  1:305916/250‑1 (MQ=255)                                                                                                                                                                     ggTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTgg                                                                      >  1:122476/1‑250 (MQ=255)                                                                                                                                                                         gTGAGTATTTGATGTAGCGTTATAAATTACCGCGGGATGAGTACCCGAAAGGCTGCGGCGAGCAGCTGGCGAATTGGCA‑TAAAGAGATGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGGCCCGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTa                                                                 <  2:7191/251‑1 (MQ=255)                                                                                                                                                                             ggATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCt                                                             >  1:80640/1‑251 (MQ=255)                                                                                                                                                                                tttGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAg                                                                                  <  2:178867/227‑1 (MQ=255)                                                                                                                                                                                tttGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAg                                                                                  >  1:178867/1‑227 (MQ=255)                                                                                                                                                                                  tgattgaGCGATATAAAGTACCGCTGGATGAGTAACCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACCATCATGAACGCTATCTGGACTGGCGAGACGAGTGTGATTTACGCCCACGTCCGTAACGATGGTTTGATTGATAACCTTCCACAAGGATGTTGCGTGGACGTAGCCTGTCTg                                                        >  2:326007/1‑251 (MQ=255)                                                                                                                                                                                   gattgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCTAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATGGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTgg                                                       <  2:80640/251‑1 (MQ=255)                                                                                                                                                                                   gattgaGCGTTATAAAGGACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTg                                                        <  1:219456/250‑1 (MQ=255)                                                                                                                                                                                          cgttcaaacggcccGCTGGATAAATCCCCGAAACGCTGCGTAGAGGAGCTG‑CCAAGTTGCAATAAAGTGCTGGAGGAGTCTAAAACAGCCTCCCGAATTGATATTAACACGCCACGGGAATATGCAAGAAACCTCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTCCGGCAACGTCCGTAACGCTGGTTTGATTAATAACATGCCACAAGGATGTTGCGTGGCAGTAGCCTGTCTGGTTGATGc                                                >  2:399650/13‑251 (MQ=255)                                                                                                                                                                                                     cccGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTc                                     <  1:240018/250‑1 (MQ=255)                                                                                                                                                                                                       cGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTc                                     >  1:301721/1‑249 (MQ=255)                                                                                                                                                                                                       cGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAg                                   <  1:161951/251‑1 (MQ=255)                                                                                                                                                                                                           ggATGAGTACCCGCAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGCTTGATAACCTGCCACAAGGATGTTGCGTGGACGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCg                                >  2:135962/1‑250 (MQ=255)                                                                                                                                                                                                             aTGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGAcc                             <  2:301721/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTCGAGCAGCTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTc         >  2:131798/1‑251 (MQ=255)                                                                                                                                                                                                                                        agcTGGCGAATTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGAGTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATATCCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTTCCTTCGCATCTg  <  1:32744/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  AACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCA‑TAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTCGCATCTG  >  NC_000913/4342584‑4343065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑