Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,337,864	C→T	V22V (GTG→GTA)	adiY ←	DNA‑binding transcriptional activator AdiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,337,864	0	C	T	100.0%	56.5 / NA	22	V22V (GTG→GTA)	adiY	DNA‑binding transcriptional activator AdiY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

GGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTCACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACA  >  NC_000913/4337662‑4338094                                                                                                                                                                                                           |                                                                                                                                                                                                                                       gggTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTgg                                                                                                                                                                                        <  1:400695/251‑1 (MQ=255)     gAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCg                                                                                                                                                                                     <  1:325434/250‑1 (MQ=255)       gTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTCAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCGTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTg                                                                                                                                                                                  <  1:371851/251‑1 (MQ=255)              cGTAGCGCTACGTTGCCATTCTGGTTTTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCTATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGTTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATcc                                                                                                                                                                           <  2:370789/251‑1 (MQ=255)                                 aCTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAg                                                                                                                                                        <  1:114321/251‑1 (MQ=255)                                                      ttATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGt                                                                                                                                      <  2:185931/248‑1 (MQ=255)                                                                      ggTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACGCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGa                                                                                                                   <  2:261707/251‑1 (MQ=255)                                                                           tCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCg                                                                                                              >  1:116058/1‑251 (MQ=255)                                                                                   gatgTCGTGACGAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTGTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCACTAAAGCAAAGCGATACCTGTAAATg                                                                                                       <  2:64660/250‑1 (MQ=255)                                                                                      gTCGTGAGTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCttttt                                                                                                                                                                                                                  >  2:262083/1‑140 (MQ=255)                                                                                      gTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTGCCCGTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGtt                                                                                                    <  1:166859/250‑1 (MQ=255)                                                                                      gTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCttttt                                                                                                                                                                                                                  <  1:262083/140‑1 (MQ=255)                                                                                                                                      gATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAACGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATACCGAGTTTTAc                                                   <  1:93502/251‑1 (MQ=255)                                                                                                                                                    cacaATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAAtt                                     <  1:86831/251‑1 (MQ=255)                                                                                                                                                     acaATTTAATAACGCAATATGGTAAGCTTTAACGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGAAATCTTTTTCAGTCACTAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAACAGACCAGTTCTCGCTAACGCAAAGCGATACCGGGAAATGGTTATATAACTGTATGATGAATATAAACATGCATACAAATAACGAGTTTTACTAATAGTACAAAtat                                    >  2:245495/1‑249 (MQ=255)                                                                                                                                                            aataaagcgctatGGTTAGCTTTAAGGCTCATAGGTTCTTTCACATTTACACTTCTCATGACATCTTTTGCAGTCAATAAAACAATACACGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAACGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAACTTTTTATAGt                             >  2:188196/11‑251 (MQ=255)                                                                                                                                                             ttaaCGACATATGGTTAGCTTTAAGGCTAATAGGTTCTTTACCATTTACACTTATCCAGACATCTTTTTCAGTCAATAAAACACTACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGAACAGTTCTCGCTAAAGCAACGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATACAAATAACGAGTTTTACTAATAGTACAAATCTTTATAGt                             >  2:89422/2‑250 (MQ=255)                                                                                                                                                                         ggTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGt                 >  1:144615/1‑250 (MQ=255)                                                                                                                                                                          gTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTAc               <  1:394381/251‑1 (MQ=255)                                                                                                                                                                                         aaTAGGTTCGTTCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACGCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATTGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTGCTCATGTACATATTTTTTTACa  <  2:169860/249‑1 (MQ=255)                                                                                                                                                                                                  tttCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAACACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTa                                                    >  1:309494/1‑190 (MQ=255)                                                                                                                                                                                                  tttCCCATTTACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACGCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTa                                                    <  2:309494/190‑1 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                                       GGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAGGCTAATAGGTTCTTTCCCATTCACCCTTATCCAGACATCTTTTTCAGTCAATAAAACAATACAAGGTTGGTCGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACA  >  NC_000913/4337662‑4338094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑