Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,301,071	C→T	A670T (GCA→ACA)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,301,071	0	C	T	100.0%	84.9 / NA	31	A670T (GCA→ACA)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/20);  total (11/20)

GTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTG  >  NC_000913/4300841‑4301300                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       gttgttTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCa                                                                                                                                                                                                                    <  1:17159/251‑1 (MQ=255)   tgttTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGCTCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGGCCAGGCTGGCa                                                                                                                                                                                                                    >  2:369280/1‑249 (MQ=255)    gttTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGc                                                                                                                                                                                                                 <  1:373836/251‑1 (MQ=255)         caccacTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTTCAGTCTGGCAAGCGGGCg                                                                                                                                                                                                            >  2:152719/1‑251 (MQ=255)                          ccAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTTTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAAGGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACgtg                                                                                                                                                                                           <  2:366944/251‑1 (MQ=255)                             gCCGGAGCTGGCAAGATGAAGACCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGAGGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGATACTTGTGCCTGGTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTg                                                                                                                                                                                        <  2:311327/251‑1 (MQ=255)                              ccGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCTTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAATCTGGCAAGCGGGCGAAAAGCttaaagacgggttgc                                                                                                                                                                                       >  1:97124/1‑237 (MQ=255)                                ggAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTTGCAAGCGGGCGAAAAGCTGGCAGACGAGTTGCTc                                                                                                                                                                                     >  1:134928/1‑251 (MQ=255)                                       gCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGCTGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCCGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGGGTTGCTCCTGtttt                                                                                                                                                                              >  2:65724/1‑251 (MQ=255)                                       gCAAGATGAATATCGGCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTTATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGtttt                                                                                                                                                                              <  1:191035/251‑1 (MQ=255)                                        cAAGATGAATATCGTCGGCCAGGTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGGCCAGTCTGGCAAGCGGGCGAAAAGATGGCAGACGGGGTGTTCCTGtttt                                                                                                                                                                              >  1:117436/1‑250 (MQ=255)                                            aTGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaataa                                                                                                                                                                         <  1:22813/251‑1 (MQ=255)                                                    cgtcgGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCgggg                                                                                                                                                                                                                                   >  2:95287/1‑185 (MQ=255)                                                    cgtcgGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCgggg                                                                                                                                                                                                                                   <  1:95287/185‑1 (MQ=255)                                                            aGTTTGATTTGATCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTATGGCAAAGGGGCGAAAAGCGGGCAGAAGGGGTGCTCCTGTTTTAATAAGGTGGGCAGGAGGGcc                                                                                                                                                         >  2:300531/1‑251 (MQ=255)                                                                 gATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTgaga                                                                                                                                                    <  1:97529/251‑1 (MQ=255)                                                                     tGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGGTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCGGAGAGGc                                                                                                                                                 <  2:250192/250‑1 (MQ=255)                                                                          cGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGACAGTTGACGATTGCTCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTAGCAGACGTGTTGCACCTTTTTTCATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtgg                                                                                                                                           >  1:403228/1‑250 (MQ=255)                                                                                        gatgaGGTGCAGAAGCATTACGTACCAGGGCACATCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGGCGTGAAAGTTGACGATTGATCATTGCGTGGCGCCTTGCGCCTGTTCCGTGGCTGGCGTTGATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGGTGGCAGACGTGGTGCTCCTGTTTTAATAAGGTGGGCAGGATCGCCTGAGAGTCGGGTGTCTCTG‑CAAGCGtt                                                                                                                             <  2:382062/250‑1 (MQ=255)                                                                                                     aTCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACg                                                                                                               <  1:36949/251‑1 (MQ=255)                                                                                                     aTCCTGACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTGCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACg                                                                                                               <  2:140710/251‑1 (MQ=255)                                                                                                      tCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGGGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCCT‑TAAGGGGTTTTTGAGGAGaaagg                                                                                                              >  1:53430/1‑248 (MQ=255)                                                                                                                        caGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGGGGGTGTCTCTT‑CAAGCGTTAGTTGAGGAGAACGGCTGAGTGCGGTTGCCAGa                                                                                            <  1:138843/251‑1 (MQ=255)                                                                                                                        caGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGa                                                                                            <  1:152719/251‑1 (MQ=255)                                                                                                                                                  gataaTGCTGCACAACAGCAGACGTGAAAGTTGATGATTGATCATTGCGTGGCTCCTTGTGCCGGGTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGGGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTc                                                                  <  2:322071/248‑1 (MQ=255)                                                                                                                                                                              aGTTGACGATTGATCATTGCGTGGCTCCTTGTGACTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGATGCGGGTGTCTCTT‑CAAGCGTTATTTGATGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGt                                      <  2:40273/251‑1 (MQ=255)                                                                                                                                                                                         gATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTTTCTTTCAGGCGT‑ATTTTAGGGGAAAGGGCGAGGGGGGTTGGCCGACCGGCCGCGGATTTTTCCAGGGGGGCGGCAAAACGACCTGCCCTGTCCGGCTGTAGTGCGGTAtt                           >  1:122702/1‑250 (MQ=255)                                                                                                                                                                                                     gCTCCTTGTTCCGGATCCGTGGCGGGCGTTGATGTCGGGGCTGTCCGGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTTCTGTTTTAATAAGGTGGGCATGATGGCGTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGACTGAGTGCGGGTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGCCCTGCTCTGTCCGGCTGTAGTTCGTTATCCCGCGTCTgcgc               <  2:283715/251‑1 (MQ=255)                                                                                                                                                                                                        ccTTGGGCCTGTTCCGGGGCGGTCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCGGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGa            <  2:356127/251‑1 (MQ=255)                                                                                                                                                                                                                cTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTgagg                                                                                                                     <  2:272115/138‑1 (MQ=255)                                                                                                                                                                                                                  gTTCCGTTGCGGGCGTTAATGTCGGGGCTGTCCAGTCTTGCAAGCGGGCGAAAAGCTTGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCGGGCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTg  <  2:183289/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       GTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTT‑CAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTG  >  NC_000913/4300841‑4301300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑