Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,469	C→T	G633D (GGC→GAC)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,469	0	C	T	97.7%	138.2 / ‑7.9	45	G633D (GGC→GAC)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (23/20);  minor base G (0/1);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

CGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGCCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCC  >  NC_000913/4297222‑4297705                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               cGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCtt                                                                                                                                                                                                                                             <  1:313043/251‑1 (MQ=255)           gCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCTTCAATCACGTACTGCTCGGCGGCGCGCTGAGCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGTACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCGT‑TACGCGAGTg                                                                                                                                                                                                                                  <  1:377016/251‑1 (MQ=255)                      gCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTCCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGCACGGAGCAGTATTTGTACTCCGGAGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGACATGTAAATCGCCACTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGCGATAATTTTGTCTT‑TACGCGAGTGCACCCAAAcc                                                                                                                                                                                                                        >  2:30223/1‑250 (MQ=255)                               cAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCAt                                                                                                                                                                                                              <  1:337205/251‑1 (MQ=255)                                    ttttgtACTCTTCAATCAAGTACTGCGCGGCGGCGCTCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGGCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CTCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACTAATGCCTCATCtt                                                                                                                                                                                                           <  2:354496/247‑1 (MQ=255)                                             cGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAg                                                                                                                                                                                                 <  1:236228/250‑1 (MQ=255)                                                       gTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGt                                                                                                                                                                                      >  1:11021/1‑251 (MQ=255)                                                            gCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCg                                                                                                                                                                                 >  1:303304/1‑251 (MQ=255)                                                                         cTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCAACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCAG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGAtttttg                                                                                                                                                                   >  2:342244/1‑248 (MQ=255)                                                                           gatcggcgatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCg                                                                                                                                                                 >  1:241621/1‑251 (MQ=255)                                                                                  gatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGGTTTCGTAATCGGGCTTAAGGTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGCGATAATTTTGTCTT‑TACGCGCGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGTCGTTTGGCGTCTTAGGTAT‑TGATTTGTGCGTAGcacg                                                                                                                                                          >  2:304291/1‑248 (MQ=255)                                                                                     cggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGt                                                                                                                                                        <  2:400162/250‑1 (MQ=255)                                                                                            aCGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGGCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATcagc                                                                                                                                                <  1:364972/251‑1 (MQ=255)                                                                                               cgAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATcagcc                                                                                                                                               >  1:103487/1‑249 (MQ=255)                                                                                                 aaCGGCGCAGTATTTGTACACCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAgcg                                                                                                                                           <  2:49908/251‑1 (MQ=255)                                                                                                  aCGGCGCAGTATTTGTACTCCGGCGTTTTCGAAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGCTTTGTGCGTAGCCAGGTTAATCAGCCAgcgc                                                                                                                                          >  1:295972/1‑251 (MQ=255)                                                                                                   cGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGcgcc                                                                                                                                         >  1:7843/1‑251 (MQ=255)                                                                                                          agctttGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTACCCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGGGCACCCAAACCAATGCCTCATCTTCAAGCCCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTg                                                                                                                                  <  2:134347/248‑1 (MQ=255)                                                                                                            aTTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGcc                                                                                                                                <  1:43988/251‑1 (MQ=255)                                                                                                              ttGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTGACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTg                                                                                                                                  >  1:134347/1‑247 (MQ=255)                                                                                                                  accccGGCTTTTTCGTAATCGGTCTAAAGTTTTCGGTAACCAGCCCGTTACAGCCACCAA‑TCCCCCACTGGTAGGTCATGTATATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACTTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGcaca                                                                                                                           <  2:106620/247‑1 (MQ=255)                                                                                                                  aCTCCGGTGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCCCCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTCCATCAGCCAGCGCCGCCAGTGCCGCACAg                                                                                                                          <  2:282047/251‑1 (MQ=255)                                                                                                                         cGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGt                                                                                                                                                                                                                                   <  2:97737/140‑1 (MQ=255)                                                                                                                         cGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGt                                                                                                                                                                                                                                   >  1:97737/1‑140 (MQ=255)                                                                                                                            tttCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTc                                                                                                                 >  1:381636/1‑250 (MQ=255)                                                                                                                                   aTCGGGCTTAAGTTTTCGGTACCCAGCTGGTTACAGGCACAAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGCAGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGAACCCAAACCAAGGCCTAATCTTCAAAACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGGGCGTAGCCAGGTTAACCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACg                                                                                                         <  2:112137/251‑1 (MQ=255)                                                                                                                                     cGGGCTTAAGTTTTCGGTAACCCGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGc                                                                                                       >  1:302320/1‑251 (MQ=255)                                                                                                                                      gggCTTAAGTTTTCGAAAACCAGCTCGTTGCAGGCCCCAA‑TCCACCACTGGTAGGTCATGTAACTCGCCCCTTTGTTCGGAAGATCGCTGAACTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTAATCGACCGGc                                                                                                       >  2:25998/1‑250 (MQ=255)                                                                                                                                       ggCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCa                                                                                                      >  1:295953/1‑250 (MQ=255)                                                                                                                                               ttttCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTg                                                                                             >  1:17401/1‑251 (MQ=255)                                                                                                                                                         ccAGCTCGTTAAATGC‑CCAACTCCACCAATGGCAGGCCATGTAAATCGCCCCTTTGTACGGCAGATCGCTAAACTG‑CGCACGGGTGATAATTTTGTATT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGAAT‑TGATTTGTGCGTAGCCCGGCTCATCAGCAAGAGCCGACAGTGCCGCACAGTTACCGGTCATCGAACGGCAGGCTTAGTGGCCAACTTc                                                                                    >  2:75451/1‑250 (MQ=255)                                                                                                                                                                attACAGGCTCCAA‑TCCTCCACTGGTAGGTCACGTAAATCGCCCCTGAGTGCGGATGATCGCTGAC‑TGTCTGTCGGGTGATAATGT‑GTTTTCTACGCGAGTTCACCCAATCCATTGCCTCTTCTTCAATTCCCGGACGTTGGGCGCCTTTGG‑ATGTTAGTGGGGCGTATCCATGGTCTTTAGCCGGTGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAGGAGTAGTGACCAACTTCACGCACCg                                                                            <  2:381087/250‑1 (MQ=255)                                                                                                                                                                            aa‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTAcc                                                                 >  2:101823/1‑250 (MQ=255)                                                                                                                                                                                          tAGGACATGTAAATCGCCACTTTGTTCGGAAGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACACAGACGTTTGGCGTCTTCGGTAT‑TCATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACCGTACAATAGGGTACtcgtc                                                   >  2:345104/1‑251 (MQ=255)                                                                                                                                                                                               cATGTAAATCGCCCCTTTGTGCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCCCCCAACCCAATGCCTCATCTTCAATCCCCAGACGTTTGGCGTCTTCGTTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTg                                               <  2:103487/250‑1 (MQ=255)                                                                                                                                                                                                       tCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGcgcc                                                                                                                                         <  1:350382/152‑1 (MQ=255)                                                                                                                                                                                                       tCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGcgcc                                                                                                                                         >  2:350382/1‑152 (MQ=255)                                                                                                                                                                                                          ccccTTTGGTCGGACGATCGCTGACCTG‑CGCACGTGTGATAATTTTGTCTT‑TACGCGTGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCACGAGTAGTGACCAACTTCACGCACCGTTGAAAGTACCATCGGGTATCCGTCGGTGGGTTTggcggtt                                   >  2:244150/1‑245 (MQ=255)                                                                                                                                                                                                                 gTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCATCATCTTCAATACCCAGACGTTTGGAGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGCGAGTTTGTCGATTGGCGCt                             >  1:171099/1‑250 (MQ=255)                                                                                                                                                                                                                         gcgcGCTGTCCTG‑CGAAGGGGTGATAATGTGGGCTT‑TACGCGAGTGCCCCAAAACCAATTCCTCATCTTCAATACCCAGACGTTGGGCGTCGTAGGTAT‑TGATGTGGGCGTAGCCAGTTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCATCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCTCTACCCAGttg                    <  2:51249/248‑3 (MQ=255)                                                                                                                                                                                                                         caTCGCTGCCCTG‑CGCACGGGTGATAATTTTGTCTT‑TACGCGATTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCTTCTTCGGTAT‑TGATTTGTGCGTAGCCAGTTTCATCAGCCAGCGCCGCCAGTGCCGCACGGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCg                    <  1:143861/250‑1 (MQ=255)                                                                                                                                                                                                                                     g‑cgcACGGGTGATAATTTTGCCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATGCCCAGACTTTTGGCGTCTGCGGTAT‑TGAGTTGTGCTTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGTTgaagaa         <  1:291307/250‑1 (MQ=255)                                                                                                                                                                                                                                      gcgtaCGGGTGATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAAGACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGGCGATTGGCGCTACCCAGGCGCAGGTGAAGAAc        <  1:155245/247‑1 (MQ=255)                                                                                                                                                                                                                                         cgtgggTGATAATGTTGTCTG‑TAGGAGAGTGCCCCAAAACCAAGGTCTCATCTTCAATACCCATGCGTTGGGCGTCTTCGTTTT‑TGATTTGTGCGTAGCCAGGATCGTCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCGCCGTTGACAGTACCAGCGGGTACGCGTCGGTGAGTTTGTCGAGTGGCGCTACCCATTGGCAGGTGAATAACTgc     <  2:373497/248‑1 (MQ=255)                                                                                                                                                                                                                                             ggagATAATTTTGTCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGCAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGcc  >  1:180705/4‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               CGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAA‑TCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTG‑CGCACGGGTGATAATTTTGCCTT‑TACGCGAGTGCACCCAAACCAATGCCTCATCTTCAATACCCAGACGTTTGGCGTCTTCGGTAT‑TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCC  >  NC_000913/4297222‑4297705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑