Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,033	C→T	A447T (GCG→ACG)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,033	0	C	T	95.5%	182.3 / NA	68	A447T (GCG→ACG)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (33/31);  minor base G (1/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCAT  >  NC_000913/4285787‑4286279                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTAGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGt                                                                                                                                                                                                                                                    >  2:113845/1‑251 (MQ=255) tCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGAGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGt                                                                                                                                                                                                                                                    >  1:242063/1‑251 (MQ=255)     cAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGCAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGc                                                                                                                                                                                                                                                 >  1:172672/1‑250 (MQ=255)      aGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGCCGGTTGccc                                                                                                                                                                                                                                               >  2:326842/1‑249 (MQ=255)        aCGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGc                                                                                                                                                                                                                                             >  1:52622/1‑251 (MQ=255)              aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCgaaga                                                                                                                                                                                                                                       <  1:159528/251‑1 (MQ=255)                 ggAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACg                                                                                                                                                                                                                                    <  1:48344/251‑1 (MQ=255)                      cGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACgg                                                                                                                                                                                                                               >  2:265565/1‑250 (MQ=255)                       gTTCAGCACGGCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCAGCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACgtg                                                                                                                                                                                                                              <  1:93607/251‑1 (MQ=255)                         tCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACgtgt                                                                                                                                                                                                                             <  1:33670/250‑1 (MQ=255)                          cAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGtt                                                                                                                                                                                                                            <  1:102215/250‑1 (MQ=255)                          cAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTg                                                                                                                                                                                                                           >  1:393117/1‑251 (MQ=255)                                   gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCGACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCgg                                                                                                                                                                                                                  <  1:171226/251‑1 (MQ=255)                                     ccACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCt                                                                                                                                                                                                                >  1:27067/1‑251 (MQ=255)                                              cGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCg                                                                                                                                                                                                       <  2:331410/251‑1 (MQ=255)                                                   aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTGCGGTGTCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAg                                                                                                                                                                                                   <  1:176942/250‑1 (MQ=255)                                                     ccAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgcgc                                                                                                                                                                                                <  1:42662/251‑1 (MQ=255)                                                       aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGcc                                                                                                                                                                                               >  1:104412/1‑250 (MQ=255)                                                                     tCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGt                                                                                                                                                                                   <  1:290222/248‑1 (MQ=255)                                                                      aaTCGCGAAGAGCACCGTCCCCACTGACATACATATTTATGACGGTGCAGAAGTAGGTCTGTTCAAAACGTTCGTCATCGCCAAACAGCGTACGCGCCTGACCAGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGGCGGTTGCACGCCGCAGAACGGAAGTGTTGCAGCACCGGCTTTCAGCGCGGTATCGCCCGGCCGCGGGGCGATc                                                                                                                                                                               >  2:40319/2‑251 (MQ=255)                                                                         cgcgACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATg                                                                                                                                                                            >  1:139629/1‑251 (MQ=255)                                                                            gACGCGCGCCGTCGCCGCTGAAATACATAGTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAGCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTGCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCATCTTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCAAGaaa                                                                                                                                                                         <  1:373250/251‑1 (MQ=255)                                                                                  cgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTCCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGGCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAGccgccg                                                                                                                                                                   <  1:70668/251‑1 (MQ=255)                                                                                    ccGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGt                                                                                                                                                                 >  1:67642/1‑251 (MQ=255)                                                                                          ccgcTGAAATACATATTTTGGAAGGTGGAGAAGTGGGTCTTTTCAAAACGTTCGTGATCGCCAAACAGCGTACCCGCCTGCCCCGGCCAGGGGTCGGTGTTTCCCAGGCTCCCTTCGGGGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTTGCACGCCGAAGAACGGACGGTTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGt                                                                                                                                                           <  2:52622/251‑1 (MQ=255)                                                                                           cgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGCTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTc                                                                                                                                                          >  1:343499/1‑251 (MQ=255)                                                                                               gAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAACCAGCGTACGCGCATGACCCGGCCCGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCCGCGGGTTACCTTCGTTATCGACCAGCGTAGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCCGCTCGGTAGGGACAGACAGCGGGGTGATCAAAAAACAGCCGGTTTCGTTCTGcc                                                                                                                                                      >  2:318286/1‑251 (MQ=255)                                                                                                  atatatatTTTTGAAGGTGGAGAAGTATGTATGCTCAAACCTTTCGTGATCGCCAAACCGCGTTCGCCCCTGCCCCGGCCAGGAGTCGGTGATTACCAGGCTCCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCCCCAGGCAGCGGGGGGTTCATGAAACCGCCGGTTTCGGTCTGccacc                                                                                                                                                   <  1:220648/247‑1 (MQ=255)                                                                                                   tACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacc                                                                                                                                                   >  2:68532/1‑250 (MQ=255)                                                                                                    aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTCCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGGGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAg                                                                                                                                                 <  1:363648/251‑1 (MQ=255)                                                                                                      atatTTTTGCAGGAGGAGAAGCAGGACTGTTAAAAACATTCGTGATCGCCAAACAGCGTACGCGCCTGACCAAGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGACCCATCCAGCGGGTTACCTTCGTTATAGCCCAGCGTCGGTTGCACGCAGACGAACGGAAGTGTTGCCGAACCGACTTTCAGCTCGGTCGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAggg                                                                                                                                               >  2:402408/1‑250 (MQ=255)                                                                                                          ttttGAAGGTGGAGAAGAAGGTCTGTTAAAAGCGTCCGGGATCGCCAAACCGCGTACCCGCCTGACCCGCCCATGAGTCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTCCCTTCGTTATCGCCCAGCGTCGGTGGCACGCCGAAGACCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGG‑‑TGCCACCAGGTATCGa                                                                                                                                          <  2:233568/250‑1 (MQ=255)                                                                                                             tGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCAGGAAACCGCCGGTTTCGGTCTGCCACCACGTATCGacc                                                                                                                                        >  2:313220/1‑251 (MQ=255)                                                                                                               aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccac                                                                                                                                      >  2:288497/1‑251 (MQ=255)                                                                                                                  gTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCgg                                                                                                                                   >  1:266383/1‑251 (MQ=255)                                                                                                                     gagaAGTAGGTCTGTTCAAACCGTCCGTGATCCCCAAACAGCTTACCCGCCGGCCCCGGCCAGGATTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAAACCGGACGTGTTGCCGACCCGCCTTTCAGCTCGTTAGCCCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACa                                                                                                                                <  2:104412/251‑1 (MQ=255)                                                                                                                             ggTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGTCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCCCCAGCGGGTTACCTTCGTTAACGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGt                                                                                                                        <  1:247299/251‑1 (MQ=255)                                                                                                                                  gTTCACAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCg                                                                                                                   >  2:204272/1‑251 (MQ=255)                                                                                                                                   ttCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCg                                                                                                                   >  1:360055/1‑250 (MQ=255)                                                                                                                                       aaaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCCCGCCGAAGAACGGACGGGTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCTCCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtctt                                                                                                              >  2:65602/1‑248 (MQ=255)                                                                                                                                          cgttcggtaTCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGTTTACCAGTCTCCCTTCGGTGGCCCCCCCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTTGCACGCCGAAGAACGGACGGGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGGGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttttttt                                                                                                           <  1:391041/243‑1 (MQ=255)                                                                                                                                                 gATCGCCAAACAGCGTACGCGCCTGCCCCGGCCAGGGGGCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGACCGGATGTGTTGCCGAACCGGCTTTCAGCTCGGGAGCGCCGGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGGATCGACCACCGGACATTTCTCGTTGCCGATTTTTTGCCAGTAc                                                                                                    <  2:263285/251‑1 (MQ=255)                                                                                                                                                 gATCGCCAAACAGCGTACGCGACTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGCTCATGACACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTCCAGTAc                                                                                                    >  2:184578/1‑251 (MQ=255)                                                                                                                                                   tCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCAAGTAccc                                                                                                  >  2:297450/1‑250 (MQ=255)                                                                                                                                                    cGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGGATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTCCCAc                                                                                                 >  2:192689/1‑251 (MQ=255)                                                                                                                                                         aaCAGCGTCCGCGCCGGACCCGGCCAGGAGTCGGTGATTACCAGGCTCCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCa                                                                                            <  1:159461/251‑1 (MQ=255)                                                                                                                                                                    cgcCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCCGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCGGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCCGTACCACTCCCACCCTTCCGGGt                                                                                 >  1:330002/1‑251 (MQ=255)                                                                                                                                                                     gcCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCACCTCCAGCGGGTTACCTTCGTTATCGACCAGAGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTCTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTCCCACGCTTCCGGGtt                                                                                >  1:150875/1‑251 (MQ=255)                                                                                                                                                                      ccTGACCCGCCCAGGTGTCGGTGTTTACCAGGCTGCCTTCTGTGGCCCCCTCCAGCGGGTTACCTCCTTTTGCGCCCTGCGTCGTTTCCACGCCGAATAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCAGGAAACCGCCGGTTTCGGTCTGCCACCAGGGATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTa                                                                               <  1:217987/251‑1 (MQ=255)                                                                                                                                                                         gACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCCCTCCCCCGCTTCCGGGTTAAtt                                                                            >  1:8721/1‑251 (MQ=255)                                                                                                                                                                                     gggtggtgtttacaaggtccccTTCGGTGGCCCCCACCAGCGGTTTTCCTTCGTTATCGCCCACCTTCGTTGGCCCGCCGAAGAACGGACGGTTTGCCGACCCGCTTTTCAGCTCGGTCCCGCTGGGCAGCGGGGTGATCATTAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTTCCCCTCCCACGCTTCCGGGTTAATTGGCTCGTCCACg                                                                <  2:287211/231‑1 (MQ=255)                                                                                                                                                                                      gTCGGTGATTACCAGGCTCCCTCCGGTGGCCCCCTCCAGCGGGTTACCTCCGTTATCGCCCAGCGTCGGTTGCACGCGGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACgg                                                               <  1:73789/251‑1 (MQ=255)                                                                                                                                                                                           tGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCCGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCg                                                          >  2:45402/1‑251 (MQ=255)                                                                                                                                                                                               tACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCgaga                                                       <  1:288497/250‑1 (MQ=255)                                                                                                                                                                                                       tACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGc                                              >  2:117785/1‑251 (MQ=255)                                                                                                                                                                                                        aCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGCACCGCGAATGCGCAGcc                                             >  2:102175/1‑250 (MQ=255)                                                                                                                                                                                                            tCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTAGCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGTCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGcgac                                           <  1:129061/249‑1 (MQ=255)                                                                                                                                                                                                                  gCCCCCTCCAGCGGGTTACCTTTGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGCCGTGTTGCCGACCCGGCTGTCAGCTCGGTAGCGCCAGCCAGCGGGGTTATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTTCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAAcggtc                                   <  1:240530/251‑1 (MQ=255)                                                                                                                                                                                                                  ccccccTCCAGCGGGTTACCTTCGTTATCGACCAGCGGCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAAcggtc                                   <  1:281856/250‑1 (MQ=255)                                                                                                                                                                                                                           aGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCGGGCAGCGGGGTGATCAGGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTc                          <  1:131275/251‑1 (MQ=255)                                                                                                                                                                                                                           aGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTc                          <  1:68532/251‑1 (MQ=255)                                                                                                                                                                                                                              gggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAt                       >  2:58050/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTAAATTGGCTCg                                                                      >  1:248254/1‑202 (MQ=255)                                                                                                                                                                                                                                      ttCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGCACGGTCGGTGCCTTCGCTCGCTTTAt               >  2:154641/1‑251 (MQ=255)                                                                                                                                                                                                                                       tcgttcgcgcctaGCGGCGGTCGCACGCCGAAGACCGGACGTGTTGCCGACCCGTCTTTCAGCTCGGTAGCGCCAGGCAGCGGGTTGATCGTGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTa                <  1:65602/237‑1 (MQ=255)                                                                                                                                                                                                                                                 ccAGCGTCGGTTGCGCGCGGAAGAACGGAGGTGTTGCCGAACCGGCTTTCAGCTCGGGAGCGCCAGGCAGGGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTTCCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGcc    <  2:278061/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aGCGTCGGTTGCACGCCGAAGAACGGAGGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCAt  <  1:117785/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCAt  >  1:252229/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCAT  >  NC_000913/4285787‑4286279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑