Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,077	C→T	A276T (GCG→ACG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,077	0	C	T	93.5%	134.4 / ‑6.6	47	A276T (GCG→ACG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (19/24);  minor base G (2/1);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACAT  >  NC_000913/4283833‑4284323                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTAcc                                                                                                                                                                                                                                                  >  1:173362/1‑251 (MQ=255)               gccCACTGCATTCGCCAGGGGAACGGCCGACATGTTGTTACCACCACACAGATGGCCCGCCGCGTCCTTATATTACGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAAAACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGGCGTACCACACATCAGTccc                                                                                                                                                                                                                                     >  2:340632/1‑250 (MQ=255)                   aCTGCATTCGAAAGGTGAAAGGACGCCATGTTGTTACAAACAATCAGATGGCCCGCAGAGTCTTTATATTCAGGATTCGCACCAACCAGCATGATCGCGACGCAGCCGATAATACAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGAGCAGCACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTCAAGACGAGCATCAGAATGAGCGGCAAGCCCGGCGAAACAAATATCAGGaccagac                                                                                                                                                                                                                                 >  2:269272/1‑250 (MQ=255)                        aTTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCTATCAGATGGCCCGCCGCGTATTTATATTCCGGATTCGCACCAAACAGCATGATCGCGCCGAAGCCGGTAATAAAGGTCAGAATATAGAAGAAGCCCATAAACCC‑GGTGGCGTAGAACAAGCTCTTACGTGCTTCGCGGCCATCGCTGACTGTAAAGAAGCGCATAAGAATGAGCGGCAAGCCCGTCGTACCAAACATCAGCcccagacccaggg                                                                                                                                                                                                                           >  2:400303/1‑249 (MQ=255)                          tcgccggggtaccGGCCGCCATTTTGTTTCCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCagaga                                                                                                                                                                                                                          <  2:271393/238‑1 (MQ=255)                                 gtgaccgtccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGcgcga                                                                                                                                                                                                                  <  2:131612/243‑2 (MQ=255)                                     aCGGCCGCCATGTTGTTACCACCAATCAGGTGGCCCGCCGCGTCTTTAGATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCTGCAAGCGCGTCGTACCAATCAGCAGTCCCAGACCTAGAGAGAGCGCGGAGAt                                                                                                                                                                                                              <  2:380132/251‑1 (MQ=255)                                       ggCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCGTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCAGCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCg                                                                                                                                                                                                            <  2:204487/251‑1 (MQ=255)                                             cATGGTGTTAACACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGGAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCCTCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGCGATCGGATCtt                                                                                                                                                                                                      >  2:129158/1‑251 (MQ=255)                                                gttgttCCCACCAATCAGATGGCCCGCCGCCTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGTAGATCGGATCTTTc                                                                                                                                                                                                    <  1:275229/250‑1 (MQ=255)                                                 ttgttACCACCAATCAGATGGACCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGGTCGGCTCTTTCAc                                                                                                                                                                                                  >  2:278987/1‑251 (MQ=255)                                                      accaccAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgcc                                                                                                                                                                                             <  1:296098/251‑1 (MQ=255)                                                                  aTGGCCCACCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAAAATAGAAGTAGCCCATAAACAC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCAGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTc                                                                                                                                                                                  >  1:285173/1‑250 (MQ=255)                                                                           cgcgTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCGTAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGCTCTTTCACCAGCCCGCCCGGCTTCATGATGTCg                                                                                                                                                                         >  2:289178/1‑250 (MQ=255)                                                                              gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGacac                                                                                                                                                                     <  1:220920/251‑1 (MQ=255)                                                                              gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGacac                                                                                                                                                                     >  2:125611/1‑251 (MQ=255)                                                                                               tCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCTGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGc                                                                                                                                                     <  2:173362/250‑1 (MQ=255)                                                                                               tCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTGACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGGCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGc                                                                                                                                                     <  1:340632/250‑1 (MQ=255)                                                                                                cGCACCAACCAGCATGATCGCGCCGAAGCCTATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTTGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGGCGACACCTTGCGGGTGTACCGcta                                                                                                                                                   <  2:244262/251‑3 (MQ=255)                                                                                                        ccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCa                                                                                                                                           <  2:111934/251‑1 (MQ=255)                                                                                                         caGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCAc                                                                                                                                          <  1:401686/251‑1 (MQ=255)                                                                                                          aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACt                                                                                                                                         >  1:104805/1‑251 (MQ=255)                                                                                                            cATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGACTGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGAACTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCACTTCACTg                                                                                                                                        >  1:218670/1‑250 (MQ=255)                                                                                                                      cagAAGCCGATAATTAAGGTCAGCATATAGACGTAGCCCATAAACCC‑GGTGGCGAAGAACACGCTCTTACGTGCCTCGCGGGCATCGCTGACTGTAATGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGAGCGGATCTTTCACCAGCCCGCCCGGCCTCATGATGTCGACACCTTTCGGTTGTTCCGCCATCGCTTCATTTAACAGAttgt                                                                                                                              <  2:249430/248‑1 (MQ=255)                                                                                                                        gAAGCCGAGAATAAAGGTCAGAATATAGAAGTATCCCATAAACCCCGGTG‑CATAGACCCCGCTCGTACGTGCTTCGCGGCCATCGCTGACTGGAAAGAAGCGCATCAGAATGTGCGGCACGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCTGAGATCGGATCTTTCACCAGCCCTCCCTGCTTCATGATGTCGACACCGTTCGGGTGTACCTCCTTCGCTTCACTGATCAGATTGTTGa                                                                                                                           <  2:242250/251‑1 (MQ=255)                                                                                                                                   taaAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCg                                                                                                                >  1:135886/1‑251 (MQ=255)                                                                                                                                                  aGAAGTAGCCCATAATCCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGTAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAcc                                                                                                 <  1:171146/251‑1 (MQ=255)                                                                                                                                                        aGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATaa                                                                                             >  2:111160/1‑249 (MQ=255)                                                                                                                                                        aGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAg                                                                                           <  1:38147/251‑1 (MQ=255)                                                                                                                                                              tAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCAt                                                                                      >  2:109484/1‑250 (MQ=255)                                                                                                                                                                        tGGCGTATAACACGCGCTTCCGTGCTACGCGGGCATCGCTGACTGAAAAGAAGCGCATCAGCATGTGCGGCAAGCACGTAGTCCCAAACATCAGTACCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCACGGCTTCATGATGGCGACACCTTTCGGGTGTACCGCCATCGCTTAACTGCACAGATTGTTGAAGCTAAAGCCGACGTGTTTTATCACCATAAAGGCCATAAAGCTGGc                                                                             >  2:168715/1‑250 (MQ=255)                                                                                                                                                                          gCGTAGAACACGCTCTTACGGTCTGCGCGGGCATCGCTGACTGGAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCTCCAGCCCGCCCGGCTTCATGATGGCGACACCTTTCGGGTGTATCGCCATCGCTTCACTGAACAGATGGTTGAAGCTAAAGCCGACGTGTTTCATCCCCATAAAGGCCATAAAGCTGGCAcc                                                                          <  2:186094/251‑1 (MQ=255)                                                                                                                                                                              aGAACACGCTCTTACGTGCGTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAAGGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaa                                                                       <  1:66228/250‑1 (MQ=255)                                                                                                                                                                                   acGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCAACACCATAAAGGCCATAAAGCTGGCACCGaacagcaa                                                                 <  1:128316/251‑1 (MQ=255)                                                                                                                                                                                      ctctTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAATCGCATCAGTCTGTGCGGCAAGCCCGTCGTACCAAACATCAGACCCAGACCCAGCGAGAGCGCGGAGATAGCATCTTTCACCAGCCCGCCCGGCTTCATGTTGTCGCCACCTTTCGGGTGTACCGCCCTCCCTTCACTGCACAGATTGTTGAAGCTAAAGCCGAAGTGTTTCATCACCATAAAGGCCATCAAGCTAGCACCGaccagcaac                                                                >  1:230498/1‑249 (MQ=255)                                                                                                                                                                                             gTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGtt                                                                                                         >  2:288136/1‑201 (MQ=255)                                                                                                                                                                                             gTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGtt                                                                                                         <  1:288136/201‑1 (MQ=255)                                                                                                                                                                                                            cGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAc                                         <  1:278987/250‑1 (MQ=255)                                                                                                                                                                                                             gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCCGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGTCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc                                       >  1:210050/1‑251 (MQ=255)                                                                                                                                                                                                             gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCAGCGCTTCACTGAACATATTGTTGAAGCTAAAGCCGACGTTTTTCATCACCATAAAGTCCATAAAGCTGGCACCGAACAGCAACAGCTCGGCTTTGATAATTTGCAccc                                       <  2:202422/251‑1 (MQ=255)                                                                                                                                                                                                                   tGTAAAGAAGCGCATCAGACTGTGCGGCACGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACCGATTGTTGCAGCTAAAGCCGACGTGTTTCATCACCATaaa                                                                                            >  2:23972/1‑192 (MQ=255)                                                                                                                                                                                                                   tGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATaaa                                                                                            <  1:23972/192‑1 (MQ=255)                                                                                                                                                                                                                      aaaGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTc                               <  1:109484/250‑1 (MQ=255)                                                                                                                                                                                                                                    gAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGccgccg                >  1:373387/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGACGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAgg          <  1:206658/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGCGCGCGGCGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGAc        >  1:311818/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cccGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGAAcat  >  2:277442/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCC‑GGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACAT  >  NC_000913/4283833‑4284323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑