Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,536	C→T	Q180Q (CAG→CAA)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,536	0	C	T	100.0%	52.1 / NA	17	Q180Q (CAG→CAA)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

AAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTATT  >  NC_000913/4282298‑4282778                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aaaGAGTTACCTCAGCCATGTATGACATATAAAAATTTGAATAATCAATGAAGGTATTTTTAATATTGGCTCTAGTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGTACATGAATGTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATAAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCTTTTTATACATATGACAGTCTTGAAAAATAGCg                                                                                                                                                                                                                                        <  1:103339/251‑1 (MQ=255)      tttACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTg                                                                                                                                                                                                                                   >  2:13584/1‑251 (MQ=255)                         ccATATAAAAATTTGAATAATCAAGGAAGGTAATTTTAATATTGGCTCTATTCATTGTTGCATTGTATAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATTGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAATAATAGCGTTTTATAGGCTGCAACCTGCaaaa                                                                                                                                                                                                                <  2:40205/251‑1 (MQ=255)                                       gAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATa                                                                                                                                                                                                   >  1:177783/1‑250 (MQ=255)                                                             tAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGAt                                                                                                                                                                             <  2:37446/250‑1 (MQ=255)                                                                tatTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGCGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCt                                                                                                                                                                         >  1:147911/1‑251 (MQ=255)                                                                   tGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTc                                                                                                                                                                      >  1:232187/1‑251 (MQ=255)                                                                                tgttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAGGGtgtg                                                                                                                                                         >  2:136923/1‑251 (MQ=255)                                                                                                                         ttAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTg                                                                                                                                                                                                                            >  2:93286/1‑143 (MQ=255)                                                                                                                         ttAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTg                                                                                                                                                                                                                            <  1:93286/143‑1 (MQ=255)                                                                                                                                       gtcaTTTTTATATGAGCGAAATTTGAGTCATCGAGAATACAATTCTCAATAAGTATTTTTTCCATTATGGCGTAATTACAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGCGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGAc                                                                                                  >  1:182757/1‑251 (MQ=255)                                                                                                                                                                                  tATCAAAAAGTATTTTTTCCATTATGTCGAAATTAAAATTCGTTGTATACATATTACAGTCTTGAAAAATAGCTTTTTGTAGGCTGCAACCTGCAAAAGTCGAATTGGAGAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCTCCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCGTAATTGACACTTc                                                        <  1:389181/250‑1 (MQ=255)                                                                                                                                                                                       aaaaGTATTTTTTCCATTATGGCGAAATTAAAATTCGGTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTACTCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACTATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCTTTAtt                                                  >  2:130365/1‑250 (MQ=255)                                                                                                                                                                                                     cATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTaa                                     >  1:3652/1‑250 (MQ=255)                                                                                                                                                                                                                     aaaTTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCt                    <  2:368445/251‑1 (MQ=255)                                                                                                                                                                                                                                ataCATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACAtt         >  1:144072/1‑251 (MQ=255)                                                                                                                                                                                                                                        tACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTattatt  <  1:157427/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   AAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTATT  >  NC_000913/4282298‑4282778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑