Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,488	C→T	P4S (CCA→TCA)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,488	0	C	T	100.0%	67.2 / NA	21	P4S (CCA→TCA)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

ATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGT  >  NC_000913/4278242‑4278706                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               aTCGTCCAGACAATAAAAGAGAAAACAGAAGCAAACTTCGGTTGAAACAGCCGCTATGATCGAAGGCTAATAGTATGCTTTTTTTACCACCCGTTTTGTATGCGCGGCGATAAACGTTTGCTTTTTTGCGACGCATCAAATTGACGCAAAACTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTACGCCACGCATCTTGAAGAAAATTAAACTCTCAGGGGATGTTTTATTATGTCTACGTCATc                                                                                                                                                                                                                            >  2:349192/1‑251 (MQ=255)     tCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGATATGATCGCCGGAAAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGAAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCACAACTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTATACTCTCAGGGGCTGTTTTCTTATGTCTACGTCATCAgcg                                                                                                                                                                                                                        >  1:56078/1‑251 (MQ=255)            aaTAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCGACGTCATCAGCGCGTACcg                                                                                                                                                                                                                 <  2:230105/251‑1 (MQ=255)              aaaaaGAGAAAAAATCAGCAAACTTCGGGTGAAAACGCCGCTATGATCGCCGTATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCTTCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACTTCGTCAGTGCGTACcggc                                                                                                                                                                                                               <  1:108054/250‑1 (MQ=255)                                              aaaaGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGGT‑TACTCTACGCC‑TGGTTTTAAAATTcaac                                                                                                                                                                             >  1:145039/1‑249 (MQ=255)                                                    cGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTaa                                                                                                                                                                                      <  2:217671/236‑1 (MQ=255)                                                    cGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGGTTaa                                                                                                                                                                                      >  1:217671/1‑236 (MQ=255)                                                        aTGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑GACGCGACGCC‑TGGTTTACAAttttcccaaggttaagg                                                                                                                                                                   >  2:189965/1‑237 (MQ=255)                                                              gCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTCGACGCC‑CGGTTTAAAATTTCACAACGGTGCAGCACTGTc                                                                                                                                                             >  1:222409/1‑251 (MQ=255)                                                              gCCGGATAATCGTTGTCTTTTTGTACCACCCGGTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAATCCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCGTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTc                                                                                                                                                             <  1:383498/251‑1 (MQ=255)                                                                                           ccGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTGGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTAACGGCGGTT‑CACTTGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGTG‑TTaacaac                                                                                                                              <  2:296014/251‑1 (MQ=255)                                                                                                    aTGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTCTgg                                                                                                                      <  2:14281/250‑1 (MQ=255)                                                                                                                aaaCGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CCCTCGGCGCCCTGGTT‑AAAATTTCTCACCCTGGGAGCACCGGCCGGCCGGAAACGGGTTGCGGGG‑TTA‑CAACGTTTCTGGCGGTGGTCTACTc                                                                                                         >  2:156326/1‑251 (MQ=255)                                                                                                                                    cGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTCGACGCC‑CGGTTTAAAATTTTACAAAGTGGGAGCACTGTCCGTCAGGGA‑GGGGTTGCCGGGGTTAA‑AAAGTTTCTGGGGATGGGCTACTCGGGCATCGTCGtttccggt                                                                                      >  1:336267/1‑244 (MQ=255)                                                                                                                                                              cAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGtt                                                                                                                                             <  1:146089/170‑1 (MQ=255)                                                                                                                                                                  aaGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTc                                                                                                                         <  2:43758/185‑1 (MQ=255)                                                                                                                                                                                       gggATTGTCCGCCACGCATCTTGACGAAAATTAACCTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CCCTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTc                                  <  2:299697/251‑1 (MQ=255)                                                                                                                                                                                                 gCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTCA‑TCGACGAC‑TGGGTTAACATTTCACAACGTGGAAGCAATGTCCGCCCGGAC‑GAGGGTGTCGGG‑TTTACAAAGTTTTTGGCGGTGGTCTTCTCGGTGATCGTCGGTCCAGGGATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGGTTTTGTTGCACACTGTTTGGTTGcccg                        >  2:141185/1‑249 (MQ=255)                                                                                                                                                                                                              aCGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGGTTAAAATTTCACCACGTGGAAGCACTGTCCGTCCGGGA‑GTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGGTTTCTTTTCAACCTGTCTGGTTGCCGGACTCGGTTCTAtt           >  1:157379/1‑250 (MQ=255)                                                                                                                                                                                                                       aaaCTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTTGACGCC‑TGGGTTAAAATTTCACAACGTGGAAACACTTTCCGTCCGGAAAGTG‑TTGCCGGG‑TTAACAACGGTTTTGGCGATGGTCTACTCGGGGATCGACGTTCCCGGTGTTTTGGGGAAAGCAGGCTTCCCGCCTGCGGGAGTTTTTGTTTCAACCCGTCTAGTTTCCTGACTTGGTTCTATCGGGAttggg  >  1:370801/1‑247 (MQ=255)                                                                                                                                                                                                                                       ttttCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTCTGGc                                                                                                                     <  1:140188/120‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               ATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTT‑CACTCGACGCC‑TGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAA‑GTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGT  >  NC_000913/4278242‑4278706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑