Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,271,130	C→T	E914E (GAG→GAA)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,271,130	0	C	T	100.0%	147.9 / NA	44	E914E (GAG→GAA)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/15);  total (29/15)

GGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTC  >  NC_000913/4270890‑4271372                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   ggAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGGGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTcgccgcaac                                                                                                                                                                                                                                          >  2:314586/1‑248 (MQ=255)   aGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGCAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGccaccc                                                                                                                                                                                                                                        >  1:369795/1‑250 (MQ=255)             ctctcGCCGGTTCGCTGCTAGTTGCGCAACTCTATTCTTTGGTGGCTGCGTCTTATCAGCCGGCGGTGAATTTACTGCCGGCAGACAACCATAAATTGCTGATGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTACCGATTACCGCATCGGCTTACGAAAGCGTGCCGTGTGTGGTGCTTCGCACTCCGCCACGGTTTCTTGCTTACCGGGGACGAGGATTTCACCGCCGCCACTGCCGCCtt                                                                                                                                                                                                                              >  2:176440/2‑251 (MQ=255)                       ttagttGCCAGATTCGCAAATCTATTATCTGGAGGATGCGTCTTATCAGCAGTCGGTGAATTTACTACCGGAAGAAAAACGTCAATTGCTGGTGCAACTCTGAACGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTCAGGAAGCGAGCAGTGTGTGATGATTCGCACTCCGCACCGGTCTCTGGCGTACCGGCGCCGCGTATTTCGCCGCCACCACTGCAGCCTTCTGGTccccg                                                                                                                                                                                                                    >  2:308739/6‑249 (MQ=255)                        tCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCCCTGCCGCCGTCTGGTCCCAgg                                                                                                                                                                                                                   >  2:281360/1‑251 (MQ=255)                                    tCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCa                                                                                                                                                                                                        <  1:358366/250‑1 (MQ=255)                                     cGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGt                                                                                                                                                                                                      >  1:164429/1‑251 (MQ=255)                                                      gtggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAACCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGATTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACg                                                                                                                                                                                     >  1:48556/1‑251 (MQ=255)                                                       tggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGGGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGt                                                                                                                                                                                    >  2:108115/1‑251 (MQ=255)                                                             cGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTTCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGTGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGCCACTCCAGTCAGCCGTTTTGATCACGTCGCGAt                                                                                                                                                                              >  2:273055/1‑251 (MQ=255)                                                                      agcagGCGGTGAATTTACTGCCGGAAGCAAACCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGCCGAGGCTTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGCCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCa                                                                                                                                                                      >  2:361932/1‑250 (MQ=255)                                                                         agGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATc                                                                                                                                                                   >  1:275703/1‑250 (MQ=255)                                                                              gTGAATATACTGCCGGAAGAAAAACGTAAATTGATGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTGCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGACCACGTCGAGATTGTGCTCAATCACcaca                                                                                                                                                             <  2:85166/251‑1 (MQ=255)                                                                                           cGGAAGACAAACGTAAACTGCAGGTGCAACTCTGACAGGAAAAGGCCGCTCAGAAACAGGCCTTAACGATTACAGCATCGGATTAAGGAAGCGTGCAGTGTGTGCTGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTAGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGACAGCGGTTTTGATCCCGTCGCGATTGTGCTAAATCACCACAACGGTATTGCCCt                                                                                                                                                >  2:196805/1‑251 (MQ=255)                                                                                           cGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGAAAATCCAGTCAGCGGTTTTGATAACGTCGAGATTGTGCTCAATAACCACAATGGTGTTGCCCt                                                                                                                                                >  2:308101/1‑251 (MQ=255)                                                                                                gaaAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATcg                                                                                                                                           >  1:279070/1‑251 (MQ=255)                                                                                                 aaaaaCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATcgc                                                                                                                                          <  1:369483/251‑1 (MQ=255)                                                                                                     aCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAg                                                                                                                                      <  1:103520/251‑1 (MQ=255)                                                                                                        tAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGttt                                                                                                                                   >  1:83306/1‑251 (MQ=255)                                                                                                          aaTTGCTGGTGCAACGCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTAt                                                                                                                                 <  1:370938/251‑1 (MQ=255)                                                                                                              gCTGGTTCAACTCTGAAAGGAAAAGGCCGCCCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGTTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTTCCGCCTTCTGGTCCCAGGGCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCCCCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCa                                                                                                                              <  1:131915/250‑1 (MQ=255)                                                                                                                  gTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGAGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACg                                                                                                                         >  2:381385/1‑251 (MQ=255)                                                                                                                    gCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTc                                                                                                                       <  2:119207/251‑1 (MQ=255)                                                                                                                      aaCTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                                                                                                                      >  2:106693/1‑250 (MQ=255)                                                                                                                                    aaGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGAGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGATCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTCTGCAGTACGACGAGCAGTTGCTGAat                                                                                                        >  2:303424/1‑250 (MQ=255)                                                                                                                                         cGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGCGCAGTTGCTGAATATCGGc                                                                                                  >  1:351542/1‑251 (MQ=255)                                                                                                                                              aGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCGTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGt                                                                                             <  1:130369/251‑1 (MQ=255)                                                                                                                                                  aGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGAGTGCAATGTGTGATGCATCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGGCGACAATCCAGTCAGCGGTTTTGATCCCGTCGAGAATGTGCTCAATCACCACAATGGTGGTGCCATGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGCAGTga                                                                                           >  2:284134/1‑248 (MQ=255)                                                                                                                                                    cGGCCTTAACGATTACAGCATCGGCTTACGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGATGAATATCGGCGCAGTGccacc                                                                                       >  2:399521/1‑247 (MQ=255)                                                                                                                                                                  aCAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcg                                                                         >  1:137440/1‑251 (MQ=255)                                                                                                                                                                  aCAGCAGCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAATTGCAGACCGGTGGTCGGCtc                                                                          <  1:348078/250‑1 (MQ=255)                                                                                                                                                                       aTCGGCTTAAGGAGGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCGGACCGGGCGTCGTCTCGTCgag                                                                    <  1:306967/251‑1 (MQ=255)                                                                                                                                                                         cGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGaa                                                                  <  2:164429/251‑1 (MQ=255)                                                                                                                                                                            cTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCCCGTCGAGAatat                                                               >  2:165644/1‑251 (MQ=255)                                                                                                                                                                                            gtgtgtGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGATTACACAGCGTCTGCCCggg                                               >  2:383153/1‑250 (MQ=255)                                                                                                                                                                                                gtgATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCcg                                           >  1:355383/1‑251 (MQ=255)                                                                                                                                                                                                   aTGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGt                                        >  1:353969/1‑251 (MQ=255)                                                                                                                                                                                                    tGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGATTTGATCACGTCGAGATTGTGCTCAATCACCACCATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTATGCCCGGTGCCGCGtt                                       >  1:16614/1‑251 (MQ=255)                                                                                                                                                                                                     gCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGtt                                       <  1:349601/250‑1 (MQ=255)                                                                                                                                                                                                          gCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGGGGTCGGCTCGTCGAGTTAAAACAGCATCTTCCCGGAGCCGCGTTTTGAcc                                 >  2:303042/1‑250 (MQ=255)                                                                                                                                                                                                             cTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGtt                              >  1:79597/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAg                    <  1:106693/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTg        <  2:79597/251‑1 (MQ=255)                                                                                                                                                                                                                                         aCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTc  <  1:161648/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   GGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTC  >  NC_000913/4270890‑4271372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑