Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,269,449	C→T	C12C (TGC→TGT)	aphA →	acid phosphatase/phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,269,449	0	C	T	100.0%	71.0 / NA	22	C12C (TGC→TGT)	aphA	acid phosphatase/phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/7);  total (15/7)

AATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATG‑AC‑ACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAA  >  NC_000913/4269227‑4269692                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      aataaACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                                                                                                                                                           <  1:42208/251‑1 (MQ=255)  ataaACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTACCGTTTGTTTATTGTTCGATCTAAACAGTCCCGCTGt                                                                                                                                                                                                                          >  2:223289/1‑251 (MQ=255)                      aaaaGACAGTAACTTATCTACTGGGTGAAATATAATCATCCTGCTTTTCATCACAAAAACCTCAGATAATCCTTCCGGTCCCCGGCAGATGTCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACGGACTATTTAGGGAAAAATATGCGCAAGATCACACATGCAATCAGTGCCGTTTGTTTATTGTTCGCGCTAAACAGTTCCGCTTTTGCCCTGGCCTGATCTCCtt                                                                                                                                                                                                      <  2:328714/251‑1 (MQ=255)                       aaaGACAGGAACGTATTTACTGGGTTAAATATCATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCACCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGAGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTc                                                                                                                                                                                                     >  1:344586/1‑251 (MQ=255)                           aCAGGCACGTATTTAGTGGGTTAAAAATACTCATCCTAATTTTCATCACCACAACCGCAGATAATCCTTCAATTCCCCGGCAGATGGAGTTATGGTAAGCTGGTTTTTGCAACACATCTCACACTACAAAGTTTCAACATACTGACTATTTAGGGAAAACTATGCGAAAGATCACACTGGCAATCAGTGCCGTTTGTTTTTTGTTCGCTCAAAACATTTACGCTTTTGCCATGGCCTCATCTCATTCAc                                                                                                                                                                                                   >  2:357994/1‑249 (MQ=255)                                       ttACTGGGTTAAATATAATCATCCTGCTTTCCATCACAAAAACCGCAGATAATCATTCCTTACCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACACATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTggg                                                                                                                                                                                     >  2:246279/1‑251 (MQ=255)                                              gTTAAATCTAATCATCATGCTTTACATCACCAAAACCGCAGATAATCCTTCATTTCACCGGCAGCTGGCGTTATGGTCAGATGGCTTTTGCAACAAATCTCACAATAAAACGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTACCCCTGGGCCTAACg                                                                                                                                                                              >  2:148594/1‑251 (MQ=255)                                                  aaTATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTACCTTTCCCCGGCAGCTGGCGTTATGGTCCGATGGTTTTTGAAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTACACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGc                                                                                                                                                                          >  1:162328/1‑251 (MQ=255)                                                           atcCTGCATTACATCCCAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGATTTTACAACACATCTCACAATAACAAGCTTCAACATACTGACTATTTAGGAAGAAATATGCTCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTACCGCTGTTGCCCTGGCCTCATCTCCTTAACCGCTTAACCGTGGGACTAACGATGCCAGGCTTGc                                                                                                                                                                 >  1:246974/1‑251 (MQ=255)                                                                          acaAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGtt                                                                                                                                                                            <  1:312434/225‑1 (MQ=255)                                                                          acaAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGtt                                                                                                                                                                            >  2:312434/1‑225 (MQ=255)                                                                                    cAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCCCAATAAAAAGTTTCAACCTACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGACAGGCTTGCTGAACCGGCACCCATTCATTGGGtt                                                                                                                                        >  2:389147/1‑251 (MQ=255)                                                                                                               tGGCGTTATGGTCCGATGGTCTTTGCCACCAATTTCCCAATAAAAAGTTTAAACATACTGACTATTTAGGGAACCATACGCGCACCATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTAGCACAACTTGAAAATAGCCTc                                                                                                             >  2:186188/1‑251 (MQ=255)                                                                                                                ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAAGAAAAATTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCt                                                                                                              <  2:118502/249‑1 (MQ=255)                                                                                                                                       gCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTg                                                                                     >  1:8696/1‑251 (MQ=255)                                                                                                                                            aaaTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCAGCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGtt                                                                                <  1:389147/251‑1 (MQ=255)                                                                                                                                                                       aCTGTCTATTTAGGGAAAAATATGCTCAAGAGCCCACAGGCAAGCAGTGCCGTTTGTTTATTGGTCGCTCTAAACAGTGCCGCTGTTGCCCGGGCCTCATCTCCTTCGCCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATGCATTGGGGTTCGGTGGCACAAATTAAAAATAGCCTCGCAGGTCTTCCGCCAATGGCGGTGGGGGTTGATATCGATG‑AC‑ACTGTACTTTTTTc                                                   <  2:331520/251‑1 (MQ=255)                                                                                                                                                                                                   agatcatgcaGGCACTCAGTACCGTTTGTTCATTGTTCGTTCTAAACAGTTCCGCTCTTGCCCTGGCCTCATCTACTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCCCCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATTGCTGTAC‑‑CGGTACTTTTTTCCAATCCGGGCTTCTTGCGGGGCaaaaa                        >  2:252496/9‑250 (MQ=255)                                                                                                                                                                                                            agccAATCATTGCCGTTTGTTTATGGTTCGCTCTACACAGTTCCGCTCTTGCCCTGGCCTCATCGCCTTCACCGCTTAACCCTGTGACTAACGTTTCCAGTCTTGCTTAACAGCCACCCAGTCATTGGGTTGCGTTCGCACAAATTCAAAATAGCCTCGCAGGGCGTCCGCCAATGTCGGTGGGGTTTGATATCGATG‑AC‑ACGGTACTTTTTTCCAGTCCTTGCTTCTGGCTCGGCAAAAAAACCCTCTCg              <  2:58554/248‑1 (MQ=255)                                                                                                                                                                                                            aGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGATGTTGACCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTAGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTTATATCGATT‑ACCACG‑TACATTTTTTCAGTCCCGGATTCCTGCGCGGCAAAAAAACCTtctcc              >  2:196599/1‑250 (MQ=255)                                                                                                                                                                                                                    aGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGGTG‑AC‑ACGGTACTTTTTTCCCGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGaa        >  1:162748/1‑249 (MQ=255)                                                                                                                                                                                                                         cGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGGTATCGATG‑GC‑CCGGTACTTTTTTCCCGTCCGGGCTTCTTGCGCGGCCAAAAACCCTTCTCGCCCGAAATCGaa  >  1:269151/1‑250 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      AATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATG‑AC‑ACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAA  >  NC_000913/4269227‑4269692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑