| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,224,483 | C→T | S219F (TCC→TTC) | metH → | cobalamin‑dependent methionine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,224,483 | 0 | C | T | 96.9% | 195.1 / NA | 65 | S219F (TCC→TTC) | metH | cobalamin‑dependent methionine synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (32/31); minor base G (2/0); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCA > NC_000913/4224236‑4224726 | aaCCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATCTCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAAGGGGGTTTGCAGCGCTGGGCGTTGAGCTGCCGGTTATGATCTCCGGCACCATCACC‑GACGCCTTCgg > 2:393563/1‑251 (MQ=255) cACGGCGTCTATTTCTCCGGGCGTCACCTATCCGGAATTTCGTAATATCACTTGTGACGGGGTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGTTGGAAGGTGGCGCGGAACTGATCCTGAGTGATACCGGTGTCAACACCCTGAACGCCAAAGCGGCGGTATTTGCGGTTAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCGGTCGAGGATGATCTCCGTCACCAGCTCC‑GACGCCTTCGTGCGCa < 2:361906/251‑1 (MQ=255) aCGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCAc < 1:285429/251‑1 (MQ=255) tttCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTGTCGAGAGTCCAACAAAGCGCTGGTGGAAGGGGGCGCGGATCTGATCCTGATTGAACCCGTTTTCGACCCCCTTAACGCCCAAGCGGCGGTAATTGAGGTGGAAACGGAGTTTGCAGCGTTGGCCGTTGAGCTGCCGCTTATGGTCTCCGGCCACAACACC‑GACGCCTGCGGGCGCACggttgccggg > 2:244879/1‑242 (MQ=255) ccGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAg < 1:300345/249‑1 (MQ=255) ggCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGCAAACGGGGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCCCGACCTCCAGGCAGACAACCGAAGCATTTTAc > 2:114809/1‑250 (MQ=255) gCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTAc < 1:262313/249‑1 (MQ=255) tAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGGTTATGATCTCCGGCACCATCACC‑GACCCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAATTTTCAACTCATTgc > 1:303004/1‑251 (MQ=255) atatCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTgcgc > 1:20707/1‑251 (MQ=255) atatCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTgcgc > 1:255324/1‑251 (MQ=255) atCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGcc > 2:306942/1‑250 (MQ=255) cACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACg < 2:291498/251‑1 (MQ=255) tttGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCg > 1:82700/1‑250 (MQ=255) tttGACGGGCTGGGGGCTGCTTAACGAGAGTCCACCAAAGCGATGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACgtc < 2:315972/249‑3 (MQ=255) ggtggCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGAcc < 1:220626/250‑1 (MQ=255) gtggCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCtt < 1:312684/251‑1 (MQ=255) gcggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTgg > 1:399247/1‑251 (MQ=255) tATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACACCTCATTGCGCCACGCCGAAGCTCTGCCCTTTGGCCTGa > 1:133776/1‑250 (MQ=255) aTCGAGAGTCCACCAAAGCGGTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACGCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAAc < 2:102057/251‑1 (MQ=255) tCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACt > 1:232412/1‑251 (MQ=255) agagTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGACTCAACCCGTTTTCGACAACCTCACCGCCAAACCAGCGGTATTTGCGGCCCAACCGGAGTCCGAAACGCAGGGTGTAGAGAAGCCCATTATGATCTCCGGCCCCCTCACC‑GACCCCTTCGGGCGCCAGCCCTACGGCCAGACCACCGAAGCATTTTACCACTCATTACCCCACGCCGCAGctctcgcatctgacctgaccaa > 1:204968/1‑231 (MQ=255) accaAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACtgtg < 2:70457/244‑1 (MQ=255) accaAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACtgtg > 1:70457/1‑244 (MQ=255) ccaAAGAGCTGGTGGAAGGTGGCGCGGATCGGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGATGGGCGTTGAGCTGCAGATTATGATCTCCGGCACCATCCCC‑GACGCCCTCGGGCGCACGCCCTCCGGGACGACCCCCGAAGCCTGTTACAACTCATTGCGCCACGCCAAAGCACTGCCCTTTGGCATGCACtgggccgcgggg > 2:214766/1‑241 (MQ=255) aaaGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGcc < 1:113705/251‑1 (MQ=255) aaGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTAAAACTCATTGAGACACGCCGAAGCCCTGACCTTTGGCCTGGACTGTGCGCTGGGGccc > 2:300301/1‑251 (MQ=255) aGCGCTGGTGGAAAGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCg < 2:320474/251‑1 (MQ=255) tggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTg > 1:77950/1‑251 (MQ=255) aTCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGc > 1:224967/1‑249 (MQ=255) cTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAgg > 1:82055/1‑250 (MQ=255) gATCCTGATTGAAACCTCTTTCGTCACCCTTAACGCCAAAGCGTCGGTATTTGCGGTTACAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCAGGGCAGACCACCGAAGCATTTTACATCTCATTGCTCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTATGTGCAGGAg < 2:332483/250‑1 (MQ=255) gATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAg < 1:281470/247‑1 (MQ=255) gATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGGGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAg < 2:303004/247‑1 (MQ=255) aTCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCt < 1:234901/251‑1 (MQ=255) ccTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGt > 1:70419/1‑251 (MQ=255) ttGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACgg > 2:322487/1‑251 (MQ=255) gAAACCGTTTTCGACACCCTTCACGCCAACGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGACGACTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACCACTCATTGCGCCACGCCGACGCTCTGCCCTTTGGCCTGAACTGTGCGCTGGGGCCCGGTGAACTGCGCCAGTAAGTGCAGGAGCTGTCACGGAt > 2:264211/1‑251 (MQ=255) aaCCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTg < 2:70419/251‑1 (MQ=255) ccGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTCCGTGCCGGAGCTGTCACGGATTGCg > 2:88656/1‑251 (MQ=255) ccGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCGGTCACGGATTGCg < 2:232412/251‑1 (MQ=255) tttCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCCCGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGGGCAGGAGCTGTCACGGATTGCGGAAt > 1:177167/1‑251 (MQ=255) gACACCCTTAACGCCAAAGCGGCGGAATTTGCGGTGAAAACGGGGTTTGAAGCGCTGGGCGGTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGGACGTGCAGGAGCTGTCACGGATTGCGGGATGCtc > 1:216058/1‑250 (MQ=255) acacCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTAc < 1:322487/251‑1 (MQ=255) gTATTTGCGGTGAAACCGAAGTTTGAAACGCTACTCGTTGAGCTGCCGATTACGATCTACGGAACAATAACCAGA‑GCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCCTTTTACAACTCATTGCGCCACGCCGAAGCTCAGACCTTTGGCCTGAACTGTGCGATGGGCCCCGATGAACTCCGCCAGTACGTACAGGAACTGACACGGCTTGCGGAATGCTACGTCACCGCTCACCCGACCGCCgg > 2:373890/1‑251 (MQ=255) ggTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCa < 1:306942/251‑1 (MQ=255) caaaCGGAGTGTGAAGCGCTGGGCGTTAAGCTGCCGATTATGATCTCCGGCACCATCACC‑GCCGCCTGCGGGCGCACGCACTCGGGGCAGACCACCGAAGCAATTTACAACTCATTGAGACACGCCGCAGCTCTGACCTTTGGCCTGAACAGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGCGCAGGAGCGGTCACGGAGTGCGGCATGCTACGTCACCGCGCACCCGAACGCCGGGcacaccaacgc > 2:12875/2‑241 (MQ=255) aaaaCGGCATTTAAAGCGCTGGGGGTTGAGCTGACGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTAAAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCCACGTCACCGCGCACCCGAACGCCGGGCTACCCACCGc > 2:235014/1‑251 (MQ=255) aaaCGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGcc > 1:82336/1‑251 (MQ=255) ggAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTAACGGCTTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTg > 1:337919/1‑251 (MQ=255) aGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGt < 1:359298/251‑1 (MQ=255) gcTGGGCGTTTAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc < 1:389032/251‑1 (MQ=255) ggCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATGTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGa < 1:393596/251‑1 (MQ=255) gCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGac > 1:321001/1‑250 (MQ=255) cGATTATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATg < 1:137955/251‑1 (MQ=255) tATGATCTCCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACGCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCa < 1:48023/250‑1 (MQ=255) tctcCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAAc > 1:148939/1‑129 (MQ=255) tctcCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAAc < 2:148939/129‑1 (MQ=255) tcCGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCACGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAc > 1:41146/1‑247 (MQ=255) ccGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCCCCGAAGCATTTTACAACTCATTGCGCCACTCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCATGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACCCGATGGCAAAACAGATa < 2:200125/251‑1 (MQ=255) ccGGCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAg < 1:88656/248‑1 (MQ=255) gCACCATCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGAAGCCCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGCACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCCAAACAGATACGt > 2:280741/1‑251 (MQ=255) ccccATCACC‑GACGCCGTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTCCCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTg < 2:82700/249‑1 (MQ=255) aTCACC‑GACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGactg > 1:67192/1‑248 (MQ=255) aCC‑GACGCCTTCGGGCTCACGCTCTCCGGGCAGACCACCGAAGGATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTGTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCGTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGgc < 2:316806/251‑1 (MQ=255) gACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCa < 1:25494/251‑1 (MQ=255) | AACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACC‑GACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCA > NC_000913/4224236‑4224726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |