Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,743	C→T	intergenic (‑256/+61)	arpA ← / ← iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,743	0	C	T	100.0%	102.6 / NA	32	intergenic (‑256/+61)	arpA/iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

CATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTG  >  NC_000913/4222498‑4222958                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        cATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTa                                                                                                                                                                                                                     <  1:92159/250‑1 (MQ=255)           ttaTGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGACGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTGTGTTAATAAGTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGGCAATGCGATTAACAGACACTCTTATTCTATTGcc                                                                                                                                                                                                           <  2:309071/248‑1 (MQ=255)                             cTATCAGAACAGCGAAAAAATTTTATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGc                                                                                                                                                                                          >  1:101596/1‑249 (MQ=255)                                tCAGAACAGTGAAACAATTTAATATGATTACAACTAACGCACTATCATACATCGCTCAATCTCATACTGCAGCAGTACCAGTTTCGGTGGTATGAGATATTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGCTTAACAGCCACTCTTATTCTATTGCCACTACGGTATGATGGGCAGact                                                                                                                                                                                     >  2:175496/1‑249 (MQ=255)                                                         gattaaaaataacaaaccctcctacaTCGCCCACTCTCATAATGCAGCCGTAAACGATTCGGTGGAATGAGATCTTGCGATTTTGTTCATAATTAACAGGTTTGATGTTTAAACATACTGCTGAGTTAATAAACGTGTGTGAAGTGCATAACATTAAGATATTTTGGCGTAAATGCGATTAAAAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGACTATTGCCTCTGCCCGCCAGAAAAAGt                                                                                                                                                            >  2:191555/26‑251 (MQ=255)                                                                      gacatatCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGGGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTAAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGACAGAAAAAGTCAGCGCATTCCAc                                                                                                                                               >  2:217251/4‑251 (MQ=255)                                                                       aaaTATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTCATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCAcc                                                                                                                                              >  1:383466/1‑251 (MQ=255)                                                                                    cGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTACGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTc                                                                                                                                 >  1:242578/1‑251 (MQ=255)                                                                                       tCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCAct                                                                                                                              >  2:95523/1‑251 (MQ=255)                                                                                                                gTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCa                                                                                                           <  2:99835/245‑1 (MQ=255)                                                                                                                gTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCa                                                                                                           >  1:99835/1‑245 (MQ=255)                                                                                                                    cGGTGGCATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATCTTTACAAATAAAGCTGAGTTAATAAACGTGTGTGACGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCAACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGACAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGcc                                                                                                 >  1:50724/1‑251 (MQ=255)                                                                                                                      gTGGCATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTAGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGTGCAGAATATTGACTCTGCCCGCAAGAAAAAGTCAGCGCATCCCACCGTACTCCAGCGTCACTTCCTTCGACGCTTTATTCACCATAGCGCCa                                                                                                >  2:315731/1‑250 (MQ=255)                                                                                                                         gAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACt                                                                                            >  1:357197/1‑251 (MQ=255)                                                                                                                          aaTGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTc                                                                                           <  1:277307/251‑1 (MQ=255)                                                                                                                              agaTCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTc                                                                                       <  1:336738/251‑1 (MQ=255)                                                                                                                                       gATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCa                                                                              <  1:95523/251‑1 (MQ=255)                                                                                                                                             gTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTa                                                                        <  1:299356/251‑1 (MQ=255)                                                                                                                                                 ataatTAAGTGGTTTGATGTTTAAAACTAATGCTGAGTTACTAAAAGTGTGTGCAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGCCACGCGGTCATCGGTAATAc                                                                    >  2:180713/1‑251 (MQ=255)                                                                                                                                                              ttGATGTTTAAAAATAAGGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTc                                                        <  1:396510/250‑1 (MQ=255)                                                                                                                                                                                   gAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCAtt                                                                                                                                                   >  1:80691/1‑138 (MQ=255)                                                                                                                                                                                   gAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCAtt                                                                                                                                                   <  2:80691/138‑1 (MQ=255)                                                                                                                                                                                        aataaAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATCAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGtt                             <  2:166972/251‑1 (MQ=255)                                                                                                                                                                                         ataaAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGACAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGtt                             >  2:303480/1‑250 (MQ=255)                                                                                                                                                                                              aGTGTGTGAAGTGTATGACATTAAGTTATTGTGGCGTCAAGGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGt                       <  2:175289/251‑1 (MQ=255)                                                                                                                                                                                                tgtgtgAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGc                     >  2:283304/1‑251 (MQ=255)                                                                                                                                                                                                        gtgtATGACCTTAAGATATTTTGGCGTCAATGCGATTAACAAACACTCTTATTCTATTGCCACTCAGGTCTGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAACTCGGTCCGGAAATAGAAATTGCAGCAAACGGTTCCAGGTGCTCATCGa              >  2:332681/1‑250 (MQ=255)                                                                                                                                                                                                           tATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAAt          >  1:211721/1‑251 (MQ=255)                                                                                                                                                                                                              gACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCACGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGCCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATac        >  1:238588/1‑250 (MQ=255)                                                                                                                                                                                                              gACATGAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTGCACGGTGCTCATCGAAAATac        <  1:283304/250‑1 (MQ=255)                                                                                                                                                                                                                   tAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGACAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTg  >  1:28919/1‑251 (MQ=255)                                                                                                                                                                                                                   tAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCt   <  1:4790/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        CATTGTTTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTG  >  NC_000913/4222498‑4222958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑