Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,219,752	C→T	S386F (TCC→TTC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,219,752	0	C	T	100.0%	160.2 / NA	50	S386F (TCC→TTC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/25);  total (25/25)

ACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACC  >  NC_000913/4219510‑4219993                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  aCAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCg                                                                                                                                                                                                                                               <  2:290733/247‑1 (MQ=255)   aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACTCCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTa                                                                                                                                                                                                                                         <  2:363526/251‑1 (MQ=255)        tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTACCGGAATTAATgg                                                                                                                                                                                                                                     >  1:341698/1‑250 (MQ=255)                     tCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCa                                                                                                                                                                                                                        <  1:394633/250‑1 (MQ=255)                           aaGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCGGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAagc                                                                                                                                                                                                                  >  1:292198/1‑250 (MQ=255)                            tgcgcagggagttCGTGGAATAGCGATGTTAGGGTTTACGCAGACGAGCTTTGATAGGGGATTCAAAGTCATCCCAGACAGGATCGCGTGGCAGAAAGAGATGTCTGCAGCACACGTTCCTGACTGATATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGACTGGCGGACAAACCGGAGTTTGACAACCTTGTGCTGGAGAAACGGCATCTTTTCCCGGCATTATTGGTATTACTGCTTCAGGAagc                                                                                                                                                                                                                  >  2:63759/12‑249 (MQ=255)                               gcCGGGTATTCGGGGAATGGTGATGTTGGTGTTTACGCTGCCGGTCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCgg                                                                                                                                                                                                             <  2:371272/251‑1 (MQ=255)                                ccGGGTATTAGTGGCATGGCGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGATTCAGGAAGCAGCgg                                                                                                                                                                                                             >  2:264155/1‑250 (MQ=255)                                        tCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATc                                                                                                                                                                                                     <  1:81568/250‑1 (MQ=255)                                         cggggAATGGTTAGGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGGCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGTAAGCAGCGGAAAAAATCa                                                                                                                                                                                                    <  2:373933/247‑1 (MQ=255)                                                 ggTGATCTTGGTGTTTACGCTGCCGAGCTTTGATCGGGTATTCAACGTCATCAAAGACAGGTTCGCGCCGCAGACAGAGATGTCTGACGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGCGTTTGAAAACTTTGGGCTGGAGAAGCGGCATATTTTCCCGGACTTAATGGAATTACTGCTTCTGGAAGCAGCGGAAAAAATCACCGATCTCg                                                                                                                                                                                           >  2:184625/1‑251 (MQ=255)                                                    gaggTTGGGGTTTGCGCTGCCGGGCTTTGATCGGGTATTCAAATTCATCAAAGACACGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTTGTGCCTGCTATCAACTGGGGAAAGAGTACGATCTCGTGGGCCGAATGTCGGACACCCAGGAGTTTGAAATCTTTGTGCTGGAGAAGCGGCATATTTTCCCGTCATTAATGGAATTACTGCTTCAGGAAGCAGCTGAAAAAATCGCCGATTTCgtc                                                                                                                                                                                         <  2:399949/247‑3 (MQ=255)                                                     aTGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCg                                                                                                                                                                                        >  1:278012/1‑250 (MQ=255)                                                          ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACaa                                                                                                                                                                                   <  1:2236/250‑1 (MQ=255)                                                               ttACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATtgt                                                                                                                                                                              <  1:68584/250‑1 (MQ=255)                                                                        cGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCAt                                                                                                                                                                    >  1:259193/1‑251 (MQ=255)                                                                                gATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCt                                                                                                                                                                                                                           >  2:142690/1‑188 (MQ=255)                                                                                gATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCt                                                                                                                                                                                                                           <  1:142690/188‑1 (MQ=255)                                                                                       tATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAgcgg                                                                                                                                                     >  1:374255/1‑251 (MQ=255)                                                                                        aTTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAgcggc                                                                                                                                                    >  1:103678/1‑251 (MQ=255)                                                                                           cAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATGCGCCATCTTTATATTGAGcggcgg                                                                                                                                                  <  2:135234/250‑1 (MQ=255)                                                                                              aGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCATCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATgg                                                                                                                                              >  1:211604/1‑251 (MQ=255)                                                                                                            ggtgcgcgCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGTATGTGGCCGCGCAATATc                                                                                                                                <  2:259193/247‑1 (MQ=255)                                                                                                                   gccgcAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAAATGGCGAAAGAGCACGATCGCGTGGGACGAATGGCGGAAACCTAGAAGTTTGAAAACTTTGCGCTGGAGAAGCGGCATATTATCCCGGCAATAATGGAATTAATGATTCAGGAAACAGCGGAAAAAATCTCCGATTTCGGCGAAACAATGGTGAATCGACATATTTATATTTAGGGGCGGATGTTGCCGCTCAATATctggctgg                                                                                                                         >  1:396683/1‑251 (MQ=255)                                                                                                                               gaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAg                                                                                                             <  1:123250/251‑1 (MQ=255)                                                                                                                                  gTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAACTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTc                                                                                                          <  1:75150/251‑1 (MQ=255)                                                                                                                                    cTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAACATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGCTGGTGCCGCTCAATATCTGGCTGGAACCAGTGGAAGGTc                                                                                                          >  1:340319/1‑249 (MQ=255)                                                                                                                                        cGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGt                                                                                                    >  2:195777/1‑251 (MQ=255)                                                                                                                                            cACGTTCGTGACTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGGGGAAGGTCAGCAGTTgcg                                                                                                >  2:54249/1‑251 (MQ=255)                                                                                                                                                      ccTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGGTGCGTGACGCCAtt                                                                                      <  2:384430/251‑1 (MQ=255)                                                                                                                                                           tATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGCCACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATAACTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTga                                                                                    >  1:160223/1‑248 (MQ=255)                                                                                                                                                           tATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACACGTGGAAGGTCAGCAGTTGCGCGACGCCATTgg                                                                                    >  2:285379/1‑247 (MQ=255)                                                                                                                                                            aTCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaaga                                                                                 >  1:246096/1‑250 (MQ=255)                                                                                                                                                                          gagCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGTAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTAt                                                                  <  1:150481/251‑1 (MQ=255)                                                                                                                                                                               cGATCGCGTGGACCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCCGCGGAAAACATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGCTGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGcc                                                             >  2:356111/1‑251 (MQ=255)                                                                                                                                                                                gATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAAAGCTATTCGcc                                                             >  1:195554/1‑250 (MQ=255)                                                                                                                                                                                 aTCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGcc                                                             >  2:197593/1‑249 (MQ=255)                                                                                                                                                                                          gCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCgct                                                  <  1:195777/251‑1 (MQ=255)                                                                                                                                                                                              caTGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGCGAAGCGGCCTATTTTCCCGGCATTAATGGAATTACTGCTTGAGGAAACAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATACGCCATCTTTATATTGAGCGGCGGGTGGTGCCGCTCAATAACTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCACTTGCAGAAAACGGGAAGGGTATTCCCCAGGTTGCCgctgca                                               >  2:50627/2‑249 (MQ=255)                                                                                                                                                                                              aaTGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAATAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAAGATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTa                                              <  1:18626/251‑1 (MQ=255)                                                                                                                                                                                                 ggCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAGGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAAc                                            <  1:319286/250‑1 (MQ=255)                                                                                                                                                                                                         cccAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTGTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATGCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCt                                   <  1:54249/251‑1 (MQ=255)                                                                                                                                                                                                            aGGAGTTTGAAAACTTTGTGCGGGGGAAGCCGCATATTTTCCCGGCATTAATTGAATTCCTGCTTCAGGAAGCAGCGGAAAAAATCGCCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGGTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTCCCCTGGc                                <  2:3050/251‑1 (MQ=255)                                                                                                                                                                                                                     aaagctgtgtgCTGGAGAAGCGGCATATTTTCCCTGCATTAAAGGAATCACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAGCTCGGCGAACAAATTGTGAGTCGACATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTCGCGCGACGCCATTGAAGAATACGGTAACTCTATTCGCCAGCTTGCCGCTGCTAACATTTGCCCTGGCGTCATGCTg                       <  2:94733/244‑1 (MQ=255)                                                                                                                                                                                                                       aaCTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGtt                     >  1:195791/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCTTTGTGCTGGAGAAGCGGCATAGTGTCCCGGCATTAATGGAATTACGGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTGTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGGGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGtt                     <  2:340319/251‑1 (MQ=255)                                                                                                                                                                                                                            tgtgCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCgctg                                                 >  1:756/1‑218 (MQ=255)                                                                                                                                                                                                                            tgtgCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCgctg                                                 <  2:756/218‑1 (MQ=255)                                                                                                                                                                                                                                     gaAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGgtg       >  1:139108/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggCATATTTTCCCGGCATTAATGGAATTACTGCTGCAGGAAGCAGCGGAAAAAATCACCGATCGCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCTCCAGCTTGCCGCTGCTAACATTTTCCCTGGCTACATGCTGTTTAAAAACTCCGGTGTCAcc  <  2:55186/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACC  >  NC_000913/4219510‑4219993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑