Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,185,777	C→T	S143F (TCC→TTC)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,185,777	0	C	T	98.3%	193.2 / ‑3.6	60	S143F (TCC→TTC)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (36/22);  total (37/23)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

TGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGG  >  NC_000913/4185535‑4186022                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      tGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACAGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTGTGCATTCTATGTgg                                                                                                                                                                                                                                               >  2:34135/1‑251 (MQ=255)      cGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCAGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATc                                                                                                                                                                                                                                          >  2:66181/1‑251 (MQ=255)          aaaaGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGa                                                                                                                                                                                                                                        >  1:130326/1‑249 (MQ=255)             aGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCAGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCg                                                                                                                                                                                                                                   <  1:66181/251‑1 (MQ=255)               aTTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGACCGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGt                                                                                                                                                                                                                                 >  1:1688/1‑251 (MQ=255)                         ccTGTGCGGTAAGAACACGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACCTCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGCACGCGTCCTGTACTTTGAATTCTCTGTGGTTATCGAAGGCGGTATGACCAAcc                                                                                                                                                                                                                       >  2:333180/1‑251 (MQ=255)                            gtgCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGGATGACCAACCTg                                                                                                                                                                                                                     <  1:296600/250‑1 (MQ=255)                                     gTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTcagc                                                                                                                                                                                                           <  2:41581/251‑1 (MQ=255)                                            cgcCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCct                                                                                                                                                                                                    <  2:319184/251‑1 (MQ=255)                                              ccTGAAACACCGTGGCGACATCGGGGAGAAGTGCGGCGTTGAAGTGACCCAGACTAACGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCGTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCCATATGCCGCTGCGCGATATCGAACGCGTACGGTCCTTTGAATTCTCTGTGGATATCGAAGGCGGTATGACCAAACTGGGACGTCAGCAGGTCctg                                                                                                                                                                                                   >  2:118626/1‑250 (MQ=255)                                                tGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGAATAAAGTACGCCGTGAGCGTATGGGACACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGGCTGCTGCTCGATATGCCGCTGAGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGAATGACCAACCTGGAACGTCAGCAGATCctgact                                                                                                                                                                                                >  1:341699/1‑251 (MQ=255)                                                   aaCACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATAGGCCGCTGCGCGATATCGAACGCGTACTGGACTTTGAATTCTATGTGGTTAGCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGAGCCTGACTGaa                                                                                                                                                                                             <  2:186635/251‑1 (MQ=255)                                                       ccGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCGGGAACGTCAGCAGAGCCTGACTGAAGAGc                                                                                                                                                                                         <  1:308217/251‑1 (MQ=255)                                                          gggCGTCATCTGTGAGAAGGGCGGCGGTGAAGTGCCCCAGCCGAAAGGAAGCCGTGAGCGTATGGGCCCCATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGt                                                                                                                                                                                      <  1:69041/250‑1 (MQ=255)                                                              gTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCt                                                                                                                                                                                  >  2:71748/1‑251 (MQ=255)                                                                     gtgAGCAGTGAGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGAGTATGGGCCAAATCGAACTGGCTTCCCCGACTGCGCAAATCTGGTTCCTGACATCGCTGCCGTCCCGTCTCGGTCTGCTGCTCGCTATGCCGCAGCGCGATATCGAACGCGTATTGTACCTTGACTTCCATGGGGTTCTCGAAGGCGGTATGACACACCTGGAACGTAAGCAAATCCTGTCTGAagagacgtctctggaggcg                                                                                                                                                                           >  1:400215/1‑236 (MQ=255)                                                                       gagaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCt                                                                                                                                                                         <  2:286489/251‑1 (MQ=255)                                                                        agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTCCTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTg                                                                                                                                                                        >  2:347477/1‑251 (MQ=255)                                                                        agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTg                                                                                                                                                                        >  1:273516/1‑251 (MQ=255)                                                                        agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTg                                                                                                                                                                        <  1:65502/251‑1 (MQ=255)                                                                         gaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGACGAGCAGTATCTGGACGCGCTgg                                                                                                                                                                       >  1:177560/1‑251 (MQ=255)                                                                               cggcgTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGt                                                                                                                                                                 >  1:36300/1‑251 (MQ=255)                                                                                    ttGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGt                                                                                                                                                            >  1:364122/1‑251 (MQ=255)                                                                                      gAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGa                                                                                                                                                          <  1:5409/251‑1 (MQ=255)                                                                                           gACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTCCTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTCTCTGGACGCGCTGGAAGAGTTCGGTGACGAAt                                                                                                                                                     >  1:173237/1‑251 (MQ=255)                                                                                           gACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAAt                                                                                                                                                     >  2:70642/1‑251 (MQ=255)                                                                                                     aaaGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGa                                                                                                                                            >  2:99187/1‑250 (MQ=255)                                                                                                               gTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTAAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGCTGGGGGCg                                                                                                                                 >  1:74928/1‑251 (MQ=255)                                                                                                                   gCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGa                                                                                                                               >  1:190838/1‑249 (MQ=255)                                                                                                                    cGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGCAGc                                                                                                                            >  1:348797/1‑251 (MQ=255)                                                                                                                         gggCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGAGATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTGCGGTGACGAATTCGACGCGAGGATGGGGGCGGAAGCAATcc                                                                                                                       <  2:273099/251‑1 (MQ=255)                                                                                                                             cacaTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGc                                                                                                                   >  2:68852/1‑251 (MQ=255)                                                                                                                              acaTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGct                                                                                                                  >  1:266167/1‑251 (MQ=255)                                                                                                                                    aaCTGGCGTCCCCGACTGCTCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCGCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGTTGATCGAAGGCGGTATGACCAACCTGGAACGTCAGTAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGTAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTctgctg                                                                                                            <  1:30687/251‑1 (MQ=255)                                                                                                                                        ggCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGTTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGTACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAATCAATCCAGTCTCTGCTGAaga                                                                                                        <  2:144897/251‑1 (MQ=255)                                                                                                                                        ggCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGa                                                                                                           >  1:325776/1‑248 (MQ=255)                                                                                                                                          cTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGc                                                                                                      >  1:196356/1‑251 (MQ=255)                                                                                                                                            tCCCCGAATGCGCAAATATGGTTCCTGAACTCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCAt                                                                                                    >  1:312776/1‑251 (MQ=255)                                                                                                                                               ccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGa                                                                                                 <  1:241033/251‑1 (MQ=255)                                                                                                                                                     gcgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGa                                                                                           <  1:234319/251‑1 (MQ=255)                                                                                                                                                      cgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTgg                                                                                            >  1:260608/1‑249 (MQ=255)                                                                                                                                                      cgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAg                                                                                          <  1:2498/251‑1 (MQ=255)                                                                                                                                                                     tgcaaTCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGTTTATCGAAGGCGCTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGAGGAATTCGACGCGAATCTGGGGGCGTCCGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAAAGTGCGaaa                                                                             >  2:6939/4‑248 (MQ=255)                                                                                                                                                                      gAAATCGCAGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCAGCGCGATATCGAACGCGTACTGTACTTTGAAGTCTATGTGGTTATCGAAGGCGGTATGACCAACCGCGAACTTCATCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTGCGGTGACGAAGTCGTCGCGAAGATGGGGGCGGAGGCAATCCAGGCGCTGCTGAAGAGCATGGTTCTGGATCaatactgggaaca                                                                            <  2:384942/249‑12 (MQ=255)                                                                                                                                                                           cGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCg                                                                      <  1:64528/250‑1 (MQ=255)                                                                                                                                                                                ccgtcacggaacagTCTGCTGCTCGAAATGCCGCAGCGAGAAATCGAACGCGTCCTGTACTTTGCATTCTCTGTGGTATTCGAAGGCGGTATGCCCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGCGTTCGGTGGCGAATTCGACGCGACGATGGGGGCGGAAGCAATCCGTGCTCTGCTTAAGAGCATGGATCTGGAGCAAGGGTGCAAAAAGCTGCATGAagt                                                                >  2:384144/14‑250 (MQ=255)                                                                                                                                                                                 cGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAag                                                                 >  2:3721/1‑249 (MQ=255)                                                                                                                                                                                    cccGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTg                                                            <  1:108062/251‑1 (MQ=255)                                                                                                                                                                                                    gctCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCg                                            <  2:266167/251‑1 (MQ=255)                                                                                                                                                                                                       cGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGaa                                          >  1:233605/1‑250 (MQ=255)                                                                                                                                                                                                         atatGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCGGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCTCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCTAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAAcc                                       <  2:386189/251‑1 (MQ=255)                                                                                                                                                                                                              ccGCTGCGCGATATCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCg                                  <  1:379530/251‑1 (MQ=255)                                                                                                                                                                                                                  tGCGCGATATCGAACGCGTACTGTACTTTGGATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCACGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAACCCAACTCCGAAACCAAGCGTaa                               >  1:223819/1‑250 (MQ=255)                                                                                                                                                                                                                          atCGAACGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGAc                      <  1:93748/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGCGTACTGTACTTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTAAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGCTGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGCAGCGCTGCACGAAACCAGCTCCGACACCAAGCGTAAAAAGCAGACCAAGc                 >  2:127654/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgTACTGTACGTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCgc               >  2:123229/1‑249 (MQ=255)                                                                                                                                                                                                                                    tactgtactTTGAATTCTATGTGGTTATCGGCGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATc            >  1:112899/1‑251 (MQ=255)                                                                                                                                                                                                                                    tactgtactTTGAATTCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGCGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATc            >  1:39257/1‑251 (MQ=255)                                                                                                                                                                                                                                     aatgtactTTGAATACTATGTGGTTCTCGCAGGTGGTATGACCAACCTGGCACGTCAGCAGATCCTGACTGAACAGCAGTATCTGGACGCGCTGGAAGAGTTCGGAGACGAATTCGACGAGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGCCCAGCTGCGTGACGAGCTGAACGAAACCAACTCCAAAACTAAGCGTACAAAGCTAACCAAGCGTAta            >  2:55165/3‑249 (MQ=255)                                                                                                                                                                                                                                              tGAATTCTATGTGGTTAACGACGGCGGTATGACCAACCTGGAACGTAAGCAGATCCTGACTGAAGCGCAGCATCTGGCCGCGCTGGAAGAGTTCGGTGACGAATTCGCCGCGAAGATGGGGGCGGACGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGTACAGCTGAGTGAAGAGATGCACGAAACCCACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAActgccgg  >  2:162507/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      TGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGG  >  NC_000913/4185535‑4186022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑