| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,139,298 | G→A | A137A (GCC→GCT) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,139,298 | 0 | G | A | 95.7% | 134.0 / ‑6.0 | 47 | A137A (GCC→GCT) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (16/28); total (17/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGG‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAG > NC_000913/4139051‑4139536 | gaTGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTTCCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCa < 1:334031/251‑1 (MQ=255) gaggagtGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTGGGTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCAGGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATc < 1:24001/246‑1 (MQ=255) gtagtGACAAATGCGGCATTCCAGGCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCTGCACCATGCTTTCTTGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTAc < 1:51342/251‑1 (MQ=255) agtGCCAAATTCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATGTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCTTACTAATGCCGTCTCCGCCCTGA‑GCATCTAcgc < 1:293391/251‑1 (MQ=255) aaaTGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTaa < 2:347847/251‑1 (MQ=255) ttCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGCGCATTTGTTGCGCTACATCTATGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAACCAGGCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTACTGAACCGTACGAATGCGGTCTCCGCCCTGC‑GCATCTACGCGGTTCACATACGAAGc > 1:389074/1‑251 (MQ=255) gtTCGACCTTATCTATAGCTGACTATACAGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGC‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGAAGCGGCGCTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGATCCTGAACCGTACGAATGCCGTATCCGCCCTGA‑GCACCTACGCGGTTAACATACGGAGCAACGTCtttt > 2:80365/1‑251 (MQ=255) gtTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTAtttt < 2:169131/251‑1 (MQ=255) tCGAACTTCTCTATAGCTTACTCTGCCGCAGGGGTGTGGAGCATTTGTTGCGCTACATCGAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTg < 1:155238/250‑1 (MQ=255) ctctATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGccc > 1:119167/1‑250 (MQ=255) aGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCgg < 1:82654/251‑1 (MQ=255) cctctACCGCAGGGGTGTTGTGCATGGGTTGCGCTACATCTAAGGGCAGGGTTGGTG‑ATTCACTTCCTGCAAGTAAACAGTCCAGCGCCCGACGCGGCGGTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGAGCGTGCATTCCTAACAGCGTTTGCAGCGCCTGAACCGTACGAATCCCGTCTCCGCCCTGA‑GCATCGACGCGTTTAACATACGGAGCAACGTAGTTGGCCCCTGCCAGTGCGTCtaat < 2:389074/250‑1 (MQ=255) acagCAGGGGTGTTGAGCAGTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAcc < 2:181214/247‑1 (MQ=255) ccGCAGTGGTGTTGAGCATTTGTTGCGCTACCTTGAAGGGCAGGGT‑GATGGTTTCTAATCCTGCCCTTAAACAGGCCAGCGCCTGACGCGTCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGG‑GCATCTCCTCGTTTAACATACGGAGCAACGTTTTTTGCCCCTGCTATTGCGGCtaagacccct < 2:318353/251‑8 (MQ=255) tGAGCATTTGTTGCGCTCCATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCATCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCt < 2:136028/250‑1 (MQ=255) aTTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCCGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtatata > 2:16891/1‑250 (MQ=255) ttgttgCGCTACAGCTAAGGGCAGGGT‑GATGTATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAca < 2:92927/250‑1 (MQ=255) gttgCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAcagc < 2:84567/250‑1 (MQ=255) gcgcTACATCTAAGGGCAGGTT‑GATGGATACACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGt < 1:299908/250‑1 (MQ=255) aCATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCa < 1:349185/249‑1 (MQ=255) tCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCCTGCTTTATGGCGCGTGCATTTCTAACAGCGTCTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt > 1:38854/1‑251 (MQ=255) aaGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCACCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagt < 1:8363/251‑1 (MQ=255) aaGGGCAGGAC‑GATGGAATCACATGCTGCCAGTAAACAGGCCAGCGCCTGACGCGGCGTTTTTAGGCTGGCTGCCAGCACAATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCAACTGAACCGTACGAATGCCGACTTCGCCCTGA‑GCACCTACGCGGTTAACATACGGCGCAAAGTATTTTGCCCCGTCCAGTGCGTCCACTAACCCTTGTGAGGCGCTATATAAAGCACAGCCAAGTatagc > 2:183119/1‑250 (MQ=255) aGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagt > 1:312330/1‑250 (MQ=255) ggT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCct > 2:75503/1‑251 (MQ=255) gT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCctc < 1:317064/251‑1 (MQ=255) cacaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCAGCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCGAATAACCCTTGTGCGGCGCTATAGACAGCAGTGCCAAGGGTAGTAATAcccgctggtttcag < 2:257138/250‑12 (MQ=255) aaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACAGACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCTGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTAt < 2:185869/250‑1 (MQ=255) gCCAGTAAACCGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCTGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTaa < 2:265080/250‑1 (MQ=255) gTAAACAGTCCAGCGCTTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtgct < 2:301678/251‑1 (MQ=255) tAAAGCTGGCTGCCAGCCCCATACTTTCTGGCGCGAGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTCCGACTGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGCCCCCGGGATTTTc > 2:266729/1‑251 (MQ=255) aaaGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTca < 1:320371/251‑1 (MQ=255) cACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GTATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGa > 1:59893/1‑250 (MQ=255) aCCATGCTTTCTGGCGCGTGCCTTTCTAATAGCGTTTGCAGATCCAGCACCGTACGAATGCAGTCTCCGACCTGA‑GCATCTACGCGGTTAACATACGGAGCACCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCTGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGACGTCCACGGGATTTTCACCACAATACCCg > 2:162537/1‑247 (MQ=255) cTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACg > 1:304656/1‑99 (MQ=255) cTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACg < 2:304656/99‑1 (MQ=255) cgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGgcgc > 2:205373/1‑251 (MQ=255) tGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCCGTGCCAAGTGTAGTAATAACCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCCGGcgctt > 2:341067/1‑251 (MQ=255) ttCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttcgct < 2:314372/250‑1 (MQ=255) aaCAGCGTTTGCAGCGCCTGAACCGTACAAATGCCGTCTCCGCC‑TGGAGCATCTACGCGGTTAACATACGGAGCAACGTATCTTGCCCCTGCCAGTGCTGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATTCCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATTCCCGGAATAGCGTCGCGCAGGCGCTTCGcttctt < 1:80365/250‑1 (MQ=255) aGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTccac > 1:53347/1‑251 (MQ=255) cGTTTGCAGCTCCTGAACCGTACGAAGGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTccacca < 1:345371/251‑1 (MQ=255) gTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAt > 1:251184/1‑251 (MQ=255) gTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCGCTTTTTGCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTGCTTCCACCAt < 2:41608/251‑1 (MQ=255) gAACCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGt < 1:75503/249‑1 (MQ=255) aaCCGTACGAATGCCGTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAACGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTcgc > 1:219646/1‑251 (MQ=255) gTCTCCGCCCTGA‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAg < 1:183084/251‑1 (MQ=255) | GATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGT‑GATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGG‑GCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAG > NC_000913/4139051‑4139536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |