Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,683	C→T	N289N (AAC→AAT)	carB →	carbamoyl‑phosphate synthetase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,683	0	C	T	100.0%	131.2 / NA	48	N289N (AAC→AAT)	carB	carbamoyl‑phosphate synthetase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/27);  total (21/27)

GAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTC  >  NC_000913/31438‑31917                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGaa                                                                                                                                                                                                                                       <  2:91340/251‑1 (MQ=255)     cGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAAc                                                                                                                                                                                                                                   <  1:71609/251‑1 (MQ=255)      gCTGATCGGCTGGAAAGAGGACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAACCGCCGATGCGATGGGCATCCACCCCGGTGACTCCATCCCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTAGAAACCGGTGTTTCCAACGTTCAGTTTGCGGTGACTCCGAAAAACg                                                                                                                                                                                                                                  <  2:336/251‑1 (MQ=255)                tGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGCTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTAGTCTggt                                                                                                                                                                                                                        >  2:336685/1‑249 (MQ=255)                  gAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCAGCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGTTTCCAACGTGCAGTTTTCGGTGAATCCGAAAAACGGTCGTCTGATTg                                                                                                                                                                                                                      <  1:126651/251‑1 (MQ=255)                       agTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCAGCGGCTGCTCTATCGAAAACTGCGATGCGATGGGCATACACGCCGGTGACTCCATCACGGTCGCGCCAGCCCAAACGCTGACCGACAAATAATAGCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTGGCGGTGAATCCGAAAAACGGTCGTCTTATTTTTATc                                                                                                                                                                                                                 <  2:209526/251‑1 (MQ=255)                                        gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGACCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCAGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCgt                                                                                                                                                                                                >  1:228577/1‑251 (MQ=255)                                            gTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTcc                                                                                                                                                                                            >  1:265537/1‑251 (MQ=255)                                                   aaaCGACAACTGCATCATCGTCTGCTCTATCGAAATCTGCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                                                                                     <  2:287900/251‑1 (MQ=255)                                                   aaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                                                                                     <  1:52314/251‑1 (MQ=255)                                                    aaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGGGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGACAAACGGTCGTCTGATTGTTCTCGACATGAAACCACGCGCGTCCCGttcttc                                                                                                                                                                                    >  1:402515/1‑251 (MQ=255)                                                    aaCGACAACTGCATCATCGGTTGCTCTATCTAAAACTTCTATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGTTGGTTCCAACGTTCAGTTTGCGGTAAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGtgcttc                                                                                                                                                                                    <  1:41036/251‑1 (MQ=255)                                                     aCGACAACTGCAACATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCATCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTAAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                                                                                   <  2:111068/251‑1 (MQ=255)                                                          aaCTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCCAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                                                                              >  1:374590/1‑251 (MQ=255)                                                              gCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc                                                                                                                                                                           <  1:169400/250‑1 (MQ=255)                                                                      gTCTGCCCTATAGAAAACTTATATGCGAAGGGCATCCACACCGGTGACTCCATCACTGTAGCGCAAGACCAAACGCTGACCGACAAAGAATATCAAATAATGCGATAA‑GCGTAGATGGCGGTACTGCGAGAAATGAGAGTTGAAAACGCTGGTTCCTACGTTCGGTTTGCGGAGAATCCGAAAAACGGACGTCTGATTGGTATCGAAATGAACACACGAGTGTCCAGTTCTTCgctactggcgacggaagc                                                                                                                                                                  >  2:344478/1‑234 (MQ=255)                                                                       tCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                                                                                 >  2:187984/1‑251 (MQ=255)                                                                           ctctATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCg                                                                                                                                                             >  2:266556/1‑251 (MQ=255)                                                                                  gAAAACTTCGATGCGTTGGGCATCCACACCGGTGTCTCCATCACTGTCGTGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGGGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGGCGTCTGATTGTTATCGACATGAACCCACGCGTGTCCCGATCTTCGGCGCTGGCGTCGAAGGGGACCGGGTTccc                                                                                                                                                      >  2:3995/1‑251 (MQ=255)                                                                                          cgatgcgatgGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCt                                                                                                                                               >  1:41266/1‑250 (MQ=255)                                                                                            atgcgatgGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaaa                                                                                                                                            <  1:295508/251‑1 (MQ=255)                                                                                                gatgGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                                                                                        >  1:318923/1‑251 (MQ=255)                                                                                                   gggCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTATTATCGAAATGATCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcg                                                                                                                                      <  2:335832/250‑1 (MQ=255)                                                                                                    ggCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcggc                                                                                                                                    <  1:178410/251‑1 (MQ=255)                                                                                                     gCATCCACACCGGTGACTCCATCACTGTCGCGCAAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGCAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGTTTGCTAAAGTGgcggcg                                                                                                                                   >  2:320169/1‑251 (MQ=255)                                                                                                         ccACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                                                                                <  1:28928/250‑1 (MQ=255)                                                                                                                 gTGACCCCATCACTGTCGCGACAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTTAATCCGAAAAACTGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTg                                                                                                                       <  2:143334/251‑1 (MQ=255)                                                                                                                               gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTACCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                                                                                           <  1:12168/249‑1 (MQ=255)                                                                                                                                 cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggggttaacccccgt                                                                                                         >  1:84540/1‑237 (MQ=255)                                                                                                                                 cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAATGGCGGGGGGGTTCACCCCcgacg                                                                                                       >  2:139113/1‑251 (MQ=255)                                                                                                                                     ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGCTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGATACTCCCTcaccggtac                                                                                                   >  1:106780/1‑243 (MQ=255)                                                                                                                                         cccAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGTAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTAACCCCTCGACGCACttgtg                                                                                               >  1:271935/1‑247 (MQ=255)                                                                                                                                                gCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGCAAATGGCGGTGGGGTAAACCCCCGACGAACTGCAGAACGAcc                                                                                        >  1:242544/1‑250 (MQ=255)                                                                                                                                                     ccGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAGCGAAATGAACCCACGCGTGTCCCGTTCTTCTGCGCTGGCGTCGAAAGGGTCCGGTTTCCTGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                                                                                   <  2:204348/251‑1 (MQ=255)                                                                                                                                                            aGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc                                                                            <  2:281484/251‑1 (MQ=255)                                                                                                                                                                      aTCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc                                                                  <  1:187984/251‑1 (MQ=255)                                                                                                                                                                       tCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTAAAACCTCGACGGACTGTGGAAAGACATCAATGGGGGaggggatccggcc                                                                 >  2:115637/1‑239 (MQ=255)                                                                                                                                                                       tCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGTACCACCTCGACGAACTGGTGAACGACATCCCTGGCgggcgacatccggcc                                                                 >  1:188607/1‑238 (MQ=255)                                                                                                                                                                             g‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTc                                                           <  1:245561/251‑1 (MQ=255)                                                                                                                                                                                         aTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTGGCCCGATTGCTAAAGTGGCGGCTAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCAt                                                <  1:171864/251‑1 (MQ=255)                                                                                                                                                                                                 gctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtg                                        <  2:14035/251‑1 (MQ=255)                                                                                                                                                                                                 gctgcGGGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGAGTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGGCGAAAGCGACCGGTTGCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGTACGGACTCCTGCGTCCTTCGAGCCGGCGATCGACTAt                                         <  2:208908/250‑1 (MQ=255)                                                                                                                                                                                                    gcGTGAAATCTGCGTGGAAACCGGTGGTTCCAACGTTCAGTTTTCGGTGAATCCGAAAAACGGTCGTCGGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACCCCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgt                                       <  2:228580/249‑1 (MQ=255)                                                                                                                                                                                                    gcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgt                                       <  1:379912/249‑1 (MQ=255)                                                                                                                                                                                                         aaaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACt                                <  1:302084/251‑1 (MQ=255)                                                                                                                                                                                                                cgtgtaaaCCGGTGGTGCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGACCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCGTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTc                         <  2:122563/246‑1 (MQ=255)                                                                                                                                                                                                                                     cGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGTTACACCCCCCACGAACTGATGCACGACACCACTGGCGGGCGGACACCGGCCTCCCTCGAGCCGTCCCTCGACTATTGGGTTTCTATAATTCCCCCCTTCaaactccaaaaat    >  1:84122/1‑240 (MQ=255)                                                                                                                                                                                                                                       ttCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGGCGAACTGATGAAAGACATCACTGGCGGACGGACTCCCGCCCCCTTCGAGCCCTCCCTCGACCATGTGGGTACTAAAATTCCTCGCTTCTACTTCGGAAAAttt  >  1:177340/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCG‑TAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTC  >  NC_000913/31438‑31917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑