Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,099	C→T	intergenic (‑130/‑223)	yifB ← / → ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,099	0	C	T	94.1%	38.1 / ‑5.9	17	intergenic (‑130/‑223)	yifB/ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (9/7);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAG  >  NC_000913/3949856‑3950340                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  tGGTAAGCACACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCTACAGTGATCGGGGCCGACTTTACTCCCAGGGCTGCGCGGGCCTGCCCAATTGACAGTGACCTATACCCTCCCTGAGTCACCATTATGTGCATACGCTCTCGCTGCTGTCGCCCGCTACTTAGTGAATTTTAGTGGCTGATTCCTGTTTGTTTGTGCAAGTGAAGGTGAATTGTTCTGGCGGAGGAATGATGCTCGCaa                                                                                                                                                                                                                                             >  1:201607/1‑250 (MQ=255)                       ccGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGAGCTGCGCGGGTATGAACCATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTACTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGAGGTTCGCAAAAATGCAGCGGGCAAAGGATGa                                                                                                                                                                                                                       >  1:65461/1‑250 (MQ=255)                                         atatGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaaca                                                                                                                                                                                                     >  1:150715/1‑250 (MQ=255)                                           atGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaacaac                                                                                                                                                                                                   <  2:125975/250‑1 (MQ=255)                                                                                aGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatttt                                                                                                                                                             <  1:187028/251‑1 (MQ=255)                                                                                                              gACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                                                                                                                                >  1:60161/1‑250 (MQ=255)                                                                                                               gcaggagcggtcggtgagtcggggttATGTGGGTAAGATATCGGTGCTGTAGGTCGAGAATTCGTGAAGTTTAGGGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGATGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCTCTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat                                                                                                                              <  2:16195/227‑1 (MQ=255)                                                                                                                    agcactcctTGAGTCCACATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTGCTGTATATTTGTGCAAGTGAAGTGTAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAAATACTATGAATGGATCAACATTAAAACTGAAATTGAATTTTTTTCTCTCACTATTTTATTTTTAAAAAACAACAAGTTATATTGAAAttatta                                                                                                                         <  2:144408/247‑1 (MQ=255)                                                                                                                                 ttgccATTTTGTTCATAAGAGATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTTTGCAAGCGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATTCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAACTATTAACCGCCTCATaa                                                                                                             <  2:192649/247‑1 (MQ=255)                                                                                                                                                 aaGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAat                                                                                             <  2:150715/250‑1 (MQ=255)                                                                                                                                                                        tAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCt                                                                      >  1:320481/1‑250 (MQ=255)                                                                                                                                                                          aTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAAACAATTTATATTGAAATTATTAAACGAATCATAAAAATCGGCCAAAAAATATCTTTTACTATTTACAAAACCTATg                                                                   >  2:188425/1‑251 (MQ=255)                                                                                                                                                                                           tcTGATTCCTGTTTATTTGTGCAAGGGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAAAATTCATCCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAAACAATTTATATTGTAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGATATCTCTTTAGGCAtt                                                  >  2:257803/2‑251 (MQ=255)                                                                                                                                                                                                             gtgCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAGCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCa                                 >  2:8136/1‑250 (MQ=255)                                                                                                                                                                                                                  aGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATACGGAGGACAACGGATGAATCACGGGGAAGGGAACAACCTTCCTATTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTCAAAAACAACAATTTATATTGAAATTATTAAACGCCTCATAAAAATCGGACAAAAAATATCTTGAACTATTTACAAAACCCATGGTACCTCTTTAGGCCTTACTTCGAACAAGAAGCAAGaaa                            >  1:357712/1‑250 (MQ=255)                                                                                                                                                                                                                             gttgttCTGGCGGTGGAATGATGTTCGCAACAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAAACTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGAc                >  1:191106/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAAGGACAGCCCTTCTACGAg  <  2:62830/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  TGGTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAG  >  NC_000913/3949856‑3950340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑