Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,292	T→C	D46D (GAT→GAC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,292	0	T	C	100.0%	149.7 / NA	45	D46D (GAT→GAC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/18);  total (27/18)

TTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACG  >  NC_000913/3927052‑3927530                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                tttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGCCAACTTGTCg                                                                                                                                                                                                                                        <  2:5776/250‑1 (MQ=255)      tGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACCACTTGTCGGGCtgt                                                                                                                                                                                                                                  >  2:182825/1‑251 (MQ=255)                  gAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGt                                                                                                                                                                                                                       <  1:219519/250‑1 (MQ=255)                     ttAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGc                                                                                                                                                                                                                     >  1:289407/1‑249 (MQ=255)                                    aaTGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAACTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTAGTGCAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGAGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGATGTGAACAAGCGGTGCAGGTAAAAGTGAAAGct                                                                                                                                                                                                    >  1:193116/1‑251 (MQ=255)                                       gATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTg                                                                                                                                                                                                 >  1:246954/1‑251 (MQ=255)                                             tGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGTGCTTAAAAAATGAAAACCGCTTACATTGCCACACAACGACAAATTAGATTCGTGACATCTCACTTTTCTCGTCCACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGCTTCTTAGCCGTGAGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGAt                                                                                                                                                                                           >  1:203999/1‑251 (MQ=255)                                                      gtCGGTTTTTGTTGCTTCATCATAAGCAACAGGACGCAGGCGTATAAAACATGAAAACCGCTTACATTGCCAAACAACGTCAAATTCGCTTCGTGACATCTCACTTTTCTCGTCAACTGCCAGAACTTATTGGGCTGATCGAAGTCCAGTCGCCGATTCTTAGCCGTTTGGGGGATGGCACGCAGGACAACTTGTCGGGCTTTGACAAAGCAGTGCAGGGAAACGTGAAGGCTCTGCCTGATACCCAGTTc                                                                                                                                                                                  >  2:117735/1‑251 (MQ=255)                                                                 ttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGctct                                                                                                                                                                                                  <  2:314919/224‑1 (MQ=255)                                                                 ttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGctct                                                                                                                                                                                                  >  1:314919/1‑224 (MQ=255)                                                                    cTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcattc                                                                                                                                                                    >  1:139191/1‑251 (MQ=255)                                                                                    aGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGGCAACTGGAAGAACGTCTTGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGaaa                                                                                                                                                    <  2:289407/251‑1 (MQ=255)                                                                                      gACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTATGGGGCTGATCGAAGTCCAGGCGCCGCTTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGGGGAAACg                                                                                                                                                  >  2:39870/1‑251 (MQ=255)                                                                                         gCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCa                                                                                                                                               <  1:349619/251‑1 (MQ=255)                                                                                          cAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACCACTTCCCCTTCGCTTCGTGAAATCTCCCTTTTCTCGTCAACTGGAAGAACGTCTGGGCCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCCGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGACGTGGAAACGTc                                                                                                                                                >  1:118952/1‑249 (MQ=255)                                                                                            ggAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGCTTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGAc                                                                                                                                            >  1:231070/1‑251 (MQ=255)                                                                                               gTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCtt                                                                                                                                         >  1:71652/1‑251 (MQ=255)                                                                                                      aaaTGAAAACCGCTTACATTGCCAAACAACGTCACATTAGCTTCGTGCAATCTCACTTTTCTCGTCAACTGGCAGCACGTCTGGGGCTGATCGAAGTCGAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGGAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTAACTGGCGAAGTGGAAACGTCAGACCTTAGGGCa                                                                                                                                   >  1:144782/1‑250 (MQ=255)                                                                                                              aCCGCTTACATTGCCAAACAACGTCAAATTAGCTGCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGTTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACtt                                                                                                                          <  1:63665/251‑1 (MQ=255)                                                                                                                 ccTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACGTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAg                                                                                                                       <  1:253836/250‑1 (MQ=255)                                                                                                                       aTTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCggg                                                                                                                 >  2:312988/1‑251 (MQ=255)                                                                                                                                      cAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTaca                                                                                                   <  1:182825/250‑1 (MQ=255)                                                                                                                                               ttCGTGAAAACTCACTTTTCCCGTCAAATGGAAGAACGCATGGGGCTGATCGAAGTCCAGGCGCCGATTATTAGCCGTGTGGGGGGTGGAACGCAGGACAACTTGTCAGGCTGTGAAACTGCGGGGCAGCTAAAAGTGAAAGCTCTGCATGATGCACAGTTGGAAGTGGTTCATTCAATGGCGGAGGAGAAAAGGCAGACATTAGGGCAACACGACTTCAGCGCGGGCGCAGGGCTGTACACGCACA‑TGa                                                                                         >  2:138501/1‑250 (MQ=255)                                                                                                                                                           cACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGACCCGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACACCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAACGTGAACGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGACGTGGTAACGTCAGACCTTAGGTCAACCCGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGccc                                                                            >  1:342411/1‑251 (MQ=255)                                                                                                                                                            tcTTTTCTCGTCACCTGGAAGAACGTCTGGGGCTGATCGAAGTCCCGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTCCACGC‑CACTGAAAGCCCTTCGcccc                                                                           >  2:262664/2‑251 (MQ=255)                                                                                                                                                            aCTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGcccc                                                                           <  1:312988/251‑1 (MQ=255)                                                                                                                                                              ttttCTCGTGAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGACGAAGGGCTGTACACGCACA‑TGAACGCCCTTCGCCCCgg                                                                         >  2:338175/1‑250 (MQ=255)                                                                                                                                                              ttttCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCg                                                                          >  1:203594/1‑250 (MQ=255)                                                                                                                                                                 tctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATg                                                                       >  1:204967/1‑250 (MQ=255)                                                                                                                                                                    cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACAAGCACA‑TGAAAGCCCTTCGCCCCGATGa                                                                      >  1:178031/1‑248 (MQ=255)                                                                                                                                                                      tCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGAcc                                                                 >  1:249396/1‑251 (MQ=255)                                                                                                                                                                        aaCTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGAAAAAGGGAAAGCTCTGCCTGATGCCCAGTTGGAAGGGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGt                                                               >  2:78558/1‑251 (MQ=255)                                                                                                                                                                             gaagaaCGTCTGGGGCTGATCGAAGCCCAGGCGCCGATTCTCAGCCGTGTGGGGGATGGCACGCAGGACCACTTGTCGGGCTGTGAAAACGCGGAGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTtc                                                          >  2:365993/1‑251 (MQ=255)                                                                                                                                                                                gaacggccggggCTAAGCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGGCGGGCTGTGAAAAAGCGGGGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCAGTCACTGGCGAAGTGGGAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAACGGCGGTACACGCATA‑TGAAAGCCCTGCGCTCCGATGAAGACCGTCTTTCTcc                                                       <  2:144782/243‑1 (MQ=255)                                                                                                                                                                                 aaCGTCTGGGGCTGATCGAAGTCCCGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCACGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCACTCACTGGCGAAGTGGAAACGTCACACCTTAGGGCAACACGGCAACAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAATCCCTGCGCGCCAATGAAGACCTTCTTACTCCg                                                      <  2:203999/251‑1 (MQ=255)                                                                                                                                                                                   cGTCTGGGGCGGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGtt                                                    <  1:308946/251‑1 (MQ=255)                                                                                                                                                                                       tGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                                                >  1:339218/1‑251 (MQ=255)                                                                                                                                                                                        ggggCTGATCGAAGTCCAGGCGCCGATTCTTATCCGTTTGGGGACTGGCACGCAGGACAACTTGTCGGTCTGTGAAAAAGCGGTGCAGGTACAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGTGCGAAGGGCTGTACACGCCCA‑TGAATGCCCTTCGCTCCGATGAAGACCGTCTTTCTCCGTTGCa                                                 <  2:281295/249‑1 (MQ=255)                                                                                                                                                                                         gggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGAACTc                                              >  2:168385/1‑251 (MQ=255)                                                                                                                                                                                              gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTc                                          <  2:203594/250‑1 (MQ=255)                                                                                                                                                                                              gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTc                                          <  1:351813/250‑1 (MQ=255)                                                                                                                                                                                              gATCGAAGGCCAGGCGCCGATTCTTAGCCGTGGGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCATACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGGCACGATGAAGACCGTCTTTCTCCGTTGCACTCGGTc                                          <  2:287666/250‑1 (MQ=255)                                                                                                                                                                                                          ggCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt                             >  1:69883/1‑251 (MQ=255)                                                                                                                                                                                                            cgcCGAGTCTTAGCCGTGGGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTgg                           <  2:242406/251‑1 (MQ=255)                                                                                                                                                                                                                                     gggCACGCAGGACAACGTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg  <  1:2920/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                TTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACA‑TGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACG  >  NC_000913/3927052‑3927530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑