Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,827,026	C→T	A606V (GCG→GTG)	recG →	ATP‑dependent DNA helicase RecG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,827,026	0	C	T	100.0%	208.6 / NA	62	A606V (GCG→GTG)	recG	ATP‑dependent DNA helicase RecG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/35);  total (27/35)

GCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGG  >  NC_000913/3826784‑3827271                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        gCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTc                                                                                                                                                                                                                                                 <  1:64717/251‑1 (MQ=255)  cGGATTAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCg                                                                                                                                                                                                                                                <  1:386026/251‑1 (MQ=255)     aTGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCt                                                                                                                                                                                                                                             <  1:208355/251‑1 (MQ=255)         aaCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCaa                                                                                                                                                                                                                                         <  1:308983/251‑1 (MQ=255)              gCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACCAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAAGTCGCCTGCAAGtgct                                                                                                                                                                                                                                    <  1:93851/251‑1 (MQ=255)               cagagaAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATGCGCCTGCAAGtgctg                                                                                                                                                                                                                                   <  1:223800/249‑1 (MQ=255)                        aGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGGCGGCGTTGATGTGCCTAACGTCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGt                                                                                                                                                                                                                          <  1:158923/251‑1 (MQ=255)                           cGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAac                                                                                                                                                                                                                       <  1:400733/251‑1 (MQ=255)                            ggTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAacg                                                                                                                                                                                                                      >  2:219555/1‑251 (MQ=255)                               gATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTGGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTTGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCTCCTGCAAGTGCTGCGCGACAGTAacgac                                                                                                                                                                                                                    <  2:201029/250‑1 (MQ=255)                               gATGGAGTCGATTAAACAAGGCGAGATACCCCTGATGGTGGCCACCACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGACAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGACTGGCGACGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGATGATCTACAAAACGCCGCTTTCTAAAACGGTGAAAATTAGCCTGCAAGTGCTGCGCGACAGTAccga                                                                                                                                                                                                                     >  2:371415/1‑249 (MQ=255)                                aTGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAacgacg                                                                                                                                                                                                                   <  2:199323/250‑1 (MQ=255)                                aTGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAacgacg                                                                                                                                                                                                                   >  1:199323/1‑250 (MQ=255)                                aTGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGGGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACgg                                                                                                                                                                                                                  >  2:194923/1‑251 (MQ=255)                                  ggcggcGTTTAAACAAGGGGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCGGTCTGATGATTAGCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACGCCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCCCTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGGGCTGCGCGACAGTAACGACGGtt                                                                                                                                                                                                                <  2:51886/251‑1 (MQ=255)                                    cgtcgtTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGtttt                                                                                                                                                                                                              <  1:288674/251‑1 (MQ=255)                                       cgtTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTtgtg                                                                                                                                                                                                           <  1:236019/251‑1 (MQ=255)                                        gtTTAAACAAGGTTAGCTACCCCTTCTGGTTGCCACAATCGTTATTGAAGTCGGCGTTGATGGGCCTATCGCCAGTCTGATGTTTATCGAAACCCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGGGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTtgtg                                                                                                                                                                                                           <  2:333171/250‑1 (MQ=255)                                               cAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCAGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCa                                                                                                                                                                                                   <  1:242986/251‑1 (MQ=255)                                                 aGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGGCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTGTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGTCAGTAACGACGGTTTTGTTATTGCGCaaa                                                                                                                                                                                                 <  2:210296/251‑1 (MQ=255)                                                  ggTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGATGATGTGCCTAACGCCAGTCTGATGATTATCGAAACCCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTAACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCaaa                                                                                                                                                                                                 >  1:385209/1‑250 (MQ=255)                                                      ggcttcaccttttagttggtacaATCGTTATTGAAGTCGTCGGTGATGTGCATGCCGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGGACTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCTCTGCGTGCTGCTCTATAAAACGACGCTTTCTAAAACGGTGTTAATTCGCCTGCAAGTGCTGCGCGACTGTATCAGCGGTTTCGTGATTGCGCAGAAAGAt                                                                                                                                                                                            <  2:116970/231‑1 (MQ=255)                                                          acacCTGCTGGTTGCCACAACCGTTATTGAAAGCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGAGTCGGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCGGCGCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTACGCGACAGTAACGACGGATTTGTGATTTGGCAACAAGATCTgg                                                                                                                                                                                        >  2:220764/1‑251 (MQ=255)                                                              ctgctgGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTgg                                                                                                                                                                                        >  1:219898/1‑247 (MQ=255)                                                                gctgGTTGCCACAACCGTTATTGAAGTCGGTGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGAt                                                                                                                                                                                    <  1:194923/249‑1 (MQ=255)                                                                    gTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCgg                                                                                                                                                                              >  1:263827/1‑251 (MQ=255)                                                                        ccACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGTTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCt                                                                                                                                                                          <  1:235763/251‑1 (MQ=255)                                                                                 ttATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAACCCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAACAGATCTGGAGATTCGCGGCCCTGGCGAATTg                                                                                                                                                                 >  1:41520/1‑251 (MQ=255)                                                                                      gAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAgg                                                                                                                                                            >  1:279547/1‑251 (MQ=255)                                                                                            ggCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCAcgcg                                                                                                                                                      >  1:364115/1‑251 (MQ=255)                                                                                            ggCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCAcgcg                                                                                                                                                      >  1:259056/1‑251 (MQ=255)                                                                                             gCGTTGATGTGCCGTACTCCAGTCGTATGATTATCGAAAACCCGGTGCGTCTGGGTCTGGCCCAGTTACTCTAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTCGCTTCTCACTGCGTGCTGCTCGACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTATCTACGGTTTTGTGATTGCGCAAGAAGAGCTGTAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGt                                                                                                                                                     <  2:243068/251‑1 (MQ=255)                                                                                                ttGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTc                                                                                                                                                    >  1:69610/1‑249 (MQ=255)                                                                                                  gATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGa                                                                                                                                                 <  2:193248/250‑1 (MQ=255)                                                                                                       gCCTAACGCCAGTCTGCTGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGt                                                                                                                                            >  1:300255/1‑250 (MQ=255)                                                                                                        ccTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACgggaa                                                                                                                                           >  2:26536/1‑248 (MQ=255)                                                                                                         cTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGAt                                                                                                                                                                                                         >  1:168279/1‑187 (MQ=255)                                                                                                         cTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGGCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCGACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTGTTGTGAt                                                                                                                                                                                                         <  2:168279/187‑1 (MQ=255)                                                                                                              gCCAGTCTGATGATTATCGAAAACCCGGAGCGGCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGTCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGa                                                                                                                                    <  2:331169/251‑1 (MQ=255)                                                                                                                         gatTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGGAATGCTGAATTTAAAGTgg                                                                                                                         >  1:261831/1‑251 (MQ=255)                                                                                                                                  aaaCCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCACATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTa                                                                                                                >  1:384342/1‑250 (MQ=255)                                                                                                                                    aCCCGGAGCGTCTGGGACTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGAAATGCTGAATTTAAAGTGGCGG‑agtttttg                                                                                                             >  2:279699/1‑244 (MQ=255)                                                                                                                                     cccGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTgc                                                                                                            >  1:221775/1‑251 (MQ=255)                                                                                                                                                       ggCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATcc                                                                                         <  2:88363/251‑1 (MQ=255)                                                                                                                                                         cgcAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGCCAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCg                                                                                       <  2:218380/251‑1 (MQ=255)                                                                                                                                                                acacCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCTAATTGTTAGGCACGCGTCAGACGGGTAATGCGGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTc                                                                                <  2:41520/251‑1 (MQ=255)                                                                                                                                                                 cacCAGCAGCGCGGGCGCGTAGGCCGTGGCGCGGTGGCTTCTCACAGCGCGCTGCACTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATAGCGCAAAAAGATATGGAGATTCGCGGCACTGGAGAATTGTTAGGAACGCGTCAGACGGGTAATGCCGAATTTCAAGTGGCGGCATTT‑CTGcgcggtaaagagatggaccccggaggccc                                                                                >  2:194998/1‑226 (MQ=255)                                                                                                                                                                     aGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTTACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAgcgc                                                                           <  1:3439/251‑1 (MQ=255)                                                                                                                                                                          cgcgGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGc                                                                      >  1:186409/1‑251 (MQ=255)                                                                                                                                                                                  cgTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCAGCTTTCTAAAACGGTGCAAATTCGCCAGCACGTGCTGCGCGAAAGCAACGACGATTTTGTGATTGCGCAAAAAGATCTGGAGCTTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGAAATGCTGAATTTAACGTGGAGG‑GTTTACTGCGCGATCAGGCC‑AAGGTACCGGAAGATCAGCACCTGGCACGCCat                                                               >  1:378410/1‑250 (MQ=255)                                                                                                                                                                                           gggCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAAc                                                     <  2:219898/250‑1 (MQ=255)                                                                                                                                                                                            ggCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGAGTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACg                                                    <  2:364115/251‑1 (MQ=255)                                                                                                                                                                                             gcgcGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGt                                                   <  1:312882/251‑1 (MQ=255)                                                                                                                                                                                                 ggTGGCTTCTCCCTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTAcc                                               <  1:45339/251‑1 (MQ=255)                                                                                                                                                                                                  gTGGCTTCTCACTGCGTGCTGCTCTAAAAAACGCCGCTTTCTACAACGGTGAAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGAGATTGCGCAAAAAGATCTGGCGATTCGCGGCCATGGCGAATTGTTAGGAACGCGTCAGACGGGAAATGCTGAATTTAAAGTGGCGG‑ACTTACCTCGCGGTCAAGCGGATG‑TCCCCGAAGATCAGCGCCTGGccagacctcatcccgaacatatccc                                              >  2:348015/1‑227 (MQ=255)                                                                                                                                                                                                    ggCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCCGGCG‑GTGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGGACGTTAccccc                                            >  2:137604/1‑249 (MQ=255)                                                                                                                                                                                                           cACTGCGTGCTGCTCGACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAgg                                      <  1:63163/250‑1 (MQ=255)                                                                                                                                                                                                            aCTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACAGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGACCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑AATTACCGCGCGATAAGGCG‑ATGATACCCGAAGTTCAGCGCCTGGTACGCCACATTCACGAAAGTTACCCacaaacagcc                                    >  2:313101/1‑245 (MQ=255)                                                                                                                                                                                                               gCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGTGCAAAGTCGCCGGCAAGTGCTGCGCGACAGTAACGACGGTTTGGTGATTGCGCAAAAAGATCTGGAGAGTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCaaaa                                 <  2:167797/251‑1 (MQ=255)                                                                                                                                                                                                                                 cgccgcTTTCTAAAACGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTgg               <  1:219555/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaaCGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGAc    <  1:70574/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaCGGTGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGAACCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACgg  >  1:392783/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        GCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGCGCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAGACGGGTAATGCTGAATTTAAAGTGGCGG‑ATTTACTGCGCGATCAGGCG‑ATGATCCCGGAAGTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGG  >  NC_000913/3826784‑3827271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑