Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	33.9 / NA	13	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/4);  total (9/4)

GCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGT  >  NC_000913/3707712‑3708160                                                                                                                                                                                                                                            |                                                                                                                                                                                                                      gCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGCGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGCTTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaa                                                                                                                                                                                                         >  1:354214/1‑250 (MQ=255)               tttggttgttTTA‑CCCTTTTCAGGGGGTTGTCGCTGCCTGACCCTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTa                                                                                                                                                                                          <  1:330633/246‑1 (MQ=255)                                      gTGGGTCGTTGCTGCATGACCTTTTTTGTTTTTTGCCCAGTCGAGTCCACGTTATGAGGTGGGGATGCCGTACTAATTACCCTCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCATCGCGACCTACAATCTGTCAACAGAATGTGAAAACGTCACTACAGACAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAACACCTTCAAACAGCTATTTGCAGCAGCGACATTTGTGCt                                                                                                                                                                   >  2:58186/1‑251 (MQ=255)                                                      tGACCTTTTTTGGTTTTGGCCCGGTCGGATCAACGTTATGAGGAGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCACTCGTGACCTACAATATGTCAACAGAATGTGAAAAAGTAAATACAGCCAACCGGGATTTACACAACCGGTGAGAATCCAAAACCACAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAAAAATTAGTGCAACTCCAACATGAcc                                                                                                                                                     >  2:328078/1‑249 (MQ=255)                                                                 gttttgtgCCTGGTCGTGTCATCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGAGCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTGACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTGGCAGCAGAGAAAGTTGTGCTACTCCAACAGGACCAGAACAATCAGc                                                                                                                                         <  1:142907/250‑1 (MQ=255)                                                                                    cAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTg                                                                                                                     <  2:228982/251‑1 (MQ=255)                                                                                            tGAGGTGGGGATGCCGTACTACTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTa                                                                                                              >  1:80590/1‑250 (MQ=255)                                                                                                                     aCATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCGACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAGGCCACAAATATAGATTTAAAAAACTTCAATCAGCGATTTGCAGCAGAGAAAGTTGTGCTTCTCCAACATGACGATAACAATCAGCTGAATAGTTAGCAACATTTGGTGATTAAGTTGTAGGCAGTTAATGAGAAAAAt                                                                                    <  2:55993/251‑1 (MQ=255)                                                                                                                         cagtgaaccagcgcgaaTCCTCCAAAAAATGCCCCTCGTGACCTACAATCTGCCAACAGACTGTGCAAACGTAAATCCAGCCAACCGAGATTTCCACCAAAGGTGAGAATCCACAAAAAACGATTAAAAAAACATCAAACAGCTATTTGCAGCAGCGAACTTTGAGCTACTCCAACATGACCAGCACAATCAGCTTAATATTTAGCAACATTTGGTGCTTAAGTTTTATGCATATAATGAAAAAATTCTg                                                                                 >  2:209548/17‑250 (MQ=255)                                                                                                                                                        ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCACTACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATAcc                                                 >  2:20987/1‑251 (MQ=255)                                                                                                                                                                           tGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGcac                               >  1:396524/1‑250 (MQ=255)                                                                                                                                                                                          aaaaCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCCCAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGCCCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAACTGCCAgg               >  1:351276/1‑251 (MQ=255)                                                                                                                                                                                                       aGCCAACCGGGCTTTAAACCAACGCTGAGAATCCACCAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAACAAATCTGAGGAAAAGGTGGATATCTGCGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGt  >  2:29867/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                      GCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGT  >  NC_000913/3707712‑3708160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑