Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,589,956	G→A	L24L (CTC→CTT)	ugpE ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,589,956	0	G	A	100.0%	213.0 / NA	64	L24L (CTC→CTT)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/26);  total (38/26)

ACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGAC  >  NC_000913/3589715‑3590198                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aCCATACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCAcc                                                                                                                                                                                                                                           <  2:154394/251‑1 (MQ=255)     gACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcgcg                                                                                                                                                                                                                                        >  1:338375/1‑250 (MQ=255)         aTGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGGGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATccc                                                                                                                                                                                                                                   <  2:308967/251‑1 (MQ=255)           ggCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                                                                                                                  >  1:291774/1‑250 (MQ=255)                 tGCCGAGAGAATCGAGACGGTAATTTTGCCGAGCGCAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAAAGGCGCGCTATTCGTGCCTCCCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCCCGTACAGCGGGAAAAGGATCACCGCGATACCGAggctc                                                                                                                                                                                                                            >  2:208096/1‑247 (MQ=255)                         gcagcGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTa                                                                                                                                                                                                                   <  2:213674/247‑1 (MQ=255)                                  cGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAat                                                                                                                                                                                                          >  1:138063/1‑251 (MQ=255)                                       aTTTTGCCGAGCGTAATGCTGACCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGt                                                                                                                                                                                                     >  1:129138/1‑251 (MQ=255)                                         tttGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTc                                                                                                                                                                                                    <  1:299165/250‑1 (MQ=255)                                          ttGCCGATCGTAATGCTGAACGCCATTACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGTTGTGTGGCGGGGATGAGCGTCATCGGCGGGGCATATACGGCCTGTGTATCCAGCGGCGCCGCTATAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGGATATCGTCa                                                                                                                                                                                                   <  1:23012/250‑1 (MQ=255)                                                     aaTGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggacgg                                                                                                                                                                                        <  1:113003/250‑1 (MQ=255)                                                     aaTGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGCGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGCTGAGCGTCATCGGCGCGGCATAGACGGCCTGTGTATCCAGCGTCGCCGCGACCAACTCCACGTACAGCGGGACAAGGATCCCCGCGATCCCGAGGATCGGCATGGTATGGCGGAATAACGTCAGACacggacggg                                                                                                                                                                                       >  2:96652/1‑250 (MQ=255)                                                      aTGCTGAACGCCATCACAAAGCTGTTAATCAACATCCGCCAGAACGGCGCGCAATTCGTGCCTACCCCGTTCACCCA‑GATGTTGGGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGGTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCTCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGtt                                                                                                                                                                                      <  2:327628/251‑1 (MQ=255)                                                      aTGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGTCGGtt                                                                                                                                                                                      <  1:279371/251‑1 (MQ=255)                                                       tGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTtc                                                                                                                                                                                     >  1:12548/1‑251 (MQ=255)                                                        gCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTtc                                                                                                                                                                                     <  2:338375/250‑1 (MQ=255)                                                               gCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGCCAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCAt                                                                                                                                                                             <  1:17652/251‑1 (MQ=255)                                                                      caAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGGTCTCAATCATTGGTAAc                                                                                                                                                                      >  2:365946/1‑251 (MQ=255)                                                                       aaaGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACg                                                                                                                                                                     <  1:58864/251‑1 (MQ=255)                                                                       aaaGCTGTTAAGCAACATCCGCAAGAAAGGCGCGCTATTCGTGACTACCCCGTTCACCCA‑GATGTTGTGGCAGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGCCGGCCTGTTTATACAGCGTCGCCGCGACAAACGCCACGTACAGCGGGCAAAGGATCACAGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTACTCAATCATTGGTAACg                                                                                                                                                                     >  2:356806/1‑251 (MQ=255)                                                                                aaGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCt                                                                                                                                                            >  1:9721/1‑251 (MQ=255)                                                                                    aaCATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGCTGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGCATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTc                                                                                                                                                         >  1:223829/1‑250 (MQ=255)                                                                                    aaCATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGATGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTc                                                                                                                                                         >  1:167826/1‑250 (MQ=255)                                                                                     aCATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCa                                                                                                                                                        >  2:11235/1‑250 (MQ=255)                                                                                       aTCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCTCCTTGCTTTCAACa                                                                                                                                                     <  1:316390/251‑1 (MQ=255)                                                                                          cgccaggccagcgcgcTATTCCTGCCTCCCCCGTTAACCCA‑GCTGCTGTGGATGTTTTACAGCAGATGTGTGCCGGGGCTGCGCGTCATCGGCGCGGCATAGACGGCCTGTTTCTCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGCTCCCGAGGCTCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATAATTGGTAACGCACCTTGCTTTCAACAt                                                                                                                                                    >  2:360418/11‑249 (MQ=255)                                                                                              aGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCgg                                                                                                                                               >  1:117847/1‑250 (MQ=255)                                                                                                 aCGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACACACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCCGCATGGTATGGCTGAATATCGTCAGCCACGGCCGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACt                                                                                                                                           >  2:375246/1‑251 (MQ=255)                                                                                                   ggCGCGCTATTCGTGCCTACCCCGTTGACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCACGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATAATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGc                                                                                                                                         >  2:46299/1‑251 (MQ=255)                                                                                                        gcTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGAGGATGAGCGTCCTCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcaccac                                                                                                                                    >  1:23156/1‑251 (MQ=255)                                                                                                          tATTCGTGCCTACCCCGTTC‑CACACGAGGCTGTGGATGTCAACCAGAAGATGCATGCAGGGGATCAGCGTCATCGGCGCCGCATAGACCGCCTGTTTATCAAGCGTCGACGCAACAAACGAAAAGTACAACGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGTATATCGTCAGCCACGGACGGTTCGCACTCATTGGTAACACACCTTGACTTCAACACAGCGGACCTGCACCACCg                                                                                                                                  >  2:204054/1‑251 (MQ=255)                                                                                                                tGCCTACCCCGTTCACACA‑GATGTTGTGGGTGTTTTCCGGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTGTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACAGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGGCAGCCACGGACGGTTCTCAATCATTGGTAACGCAACTTGCTTTCAACATAGCGGGACTGCACCACCGTCAGc                                                                                                                             >  2:193640/1‑250 (MQ=255)                                                                                                                  ccTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATAGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACg                                                                                                                          >  1:301039/1‑251 (MQ=255)                                                                                                                    tACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGAt                                                                                                                        >  1:30401/1‑251 (MQ=255)                                                                                                                        ccGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGCTGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTACCAAGAGTCGACGCGACAAACGCCACGTACAGAGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATAGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGCTGACg                                                                                                                    >  1:384957/1‑251 (MQ=255)                                                                                                                           ttCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGAGTCATCGGCGCGGCATAAACGGCCTGTTTATCCAGAGTCGCAGCGACAAACGCCAGGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATAATTGGGAACGCACCTTGCTTTCAACATAGCGGAACTGAACCACCGTCAGCACGATGACGAgg                                                                                                                 >  1:176213/1‑251 (MQ=255)                                                                                                                            tCACCCA‑GATGTTGAGGATGTTTTCCAGCAGATGTATGCCGGGGATAAGCGTCATCGGCGCGGCATAGAAGGCATGTTTATCCAGCGTCGCCGCGACAACCGCCACGTACAGCGGGAAAAGGCTCACCGCGATCCCGAGGATCCGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCAACGtaagaccgaagacgcgg                                                                                                                 >  2:54401/1‑234 (MQ=255)                                                                                                                                     aTGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAac                                                                                                        <  1:11235/251‑1 (MQ=255)                                                                                                                                          gtgGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAAcaccac                                                                                                   <  1:147120/251‑1 (MQ=255)                                                                                                                                               tGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACt                                                                                              >  2:153711/1‑251 (MQ=255)                                                                                                                                                             gggggtccGGGGATGAGCTGCACCGGCGCGGCATAGACGGACTGTTGATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGCAAAGGATCACCGCGATCCCGATGAGCAGCATGGTATGGCTGAATATCGACAGCCACGGACGGTTCTCAATCAGTGGTAACGCACCTTGCTTGCAACATATCGGAACTGCACCGCCGTCAGCACGAGGAGGAGGAATATCAACACCACCGTCTGGGCGGCATACGaa                                                                                <  2:370954/245‑1 (MQ=255)                                                                                                                                                                     gtggATGCGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGAAAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGacgca                                                                             >  2:252479/3‑242 (MQ=255)                                                                                                                                                                     ggggATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAACGGATCACCGCGATCCCGAGGATCAGCATGGTATGGATGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGAATGTGCGGCAGACGAAGcc                                                                             >  1:359423/1‑246 (MQ=255)                                                                                                                                                                      gggagtaGCGTCAGCGGCGCGGCATAGACGGACTGTTGATCCAGCGTCGCCGCGAAAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATcc                                                                       <  2:221057/245‑1 (MQ=255)                                                                                                                                                                           gAGCGTCATCGGCGCGGCATAGACGGCCTGTTTAGCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTc                                                                   <  1:208096/250‑1 (MQ=255)                                                                                                                                                                             gCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCgg                                                                <  2:199328/251‑1 (MQ=255)                                                                                                                                                                               gTCATCGGCGCGGAATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGt                                                               >  1:68646/1‑250 (MQ=255)                                                                                                                                                                                       cgcgGCATAGACGGCCTGTTTATCCAGCGTCGACGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTc                                                      >  1:352010/1‑251 (MQ=255)                                                                                                                                                                                       cgcgGCATAGACGGCCTGTTTATCCAGCGGCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGAGGACGAGGAACATCAACACCACCGACTGTGGGGCAGACGAATCCAGATCCAGTCCGGTAAAACCTTc                                                      <  2:68646/251‑1 (MQ=255)                                                                                                                                                                                         cgGCATAGACTGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGAGTCGCAATCAGTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAACCCTTcgc                                                    <  2:87819/251‑1 (MQ=255)                                                                                                                                                                                          ggCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTcgcg                                                   <  1:153711/251‑1 (MQ=255)                                                                                                                                                                                           gCATAGACGGACTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGACAGATCCAGTCCGGTAAAACCTTCGCgg                                                  >  2:380631/1‑251 (MQ=255)                                                                                                                                                                                                  cGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCt                                           >  1:304674/1‑251 (MQ=255)                                                                                                                                                                                                            aTCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAg                                 <  2:124214/251‑1 (MQ=255)                                                                                                                                                                                                            aTCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTAGAAATCAg                                 <  2:242604/251‑1 (MQ=255)                                                                                                                                                                                                             tCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGc                                >  1:180394/1‑251 (MQ=255)                                                                                                                                                                                                              ccAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTACAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGc                                <  2:4686/250‑1 (MQ=255)                                                                                                                                                                                                                  cGTCGACGCGACAACCGCCACGTACAGCGGGAAAAGGATCAACGCGATCCAGAGGATCAGCATGGTATGACTGAATATAGTCAGCCACGGACGGTTATCAGTCATTGGTAACGCAACTTGCTTTCAACATAGCGGAAATGCACCACCGTCAGCACGATGACTAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATACAGTCCGGTAAAACCTTCGCGGTAGAGCTTATAAATCAGcgtcgg                           >  1:284238/1‑250 (MQ=255)                                                                                                                                                                                                                     caccgcGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGAGCCCGAGGATCAGCATGGTATGGCTGAATACCGTCAGCTACGGACGGTTCTCAATCACTTGTAACGCACCTTGCATTCAACATAGCGGAACTGCACCACCGTCATCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCG‑TAAAACCTTCGCGGTAGATCTTATAAATCATCGTCgttgtg                       <  2:1779/249‑1 (MQ=255)                                                                                                                                                                                                                      gccgcGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCgtggtg                       >  1:384602/1‑251 (MQ=255)                                                                                                                                                                                                                               aaCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAAcc              >  1:326625/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGc           >  1:320190/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGATGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTTGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCccg        <  2:167826/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGAc  >  1:377170/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCA‑GATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGAC  >  NC_000913/3589715‑3590198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑