Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,588,787 G→A P132S (CCG→TCG)  ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,7870GA98.7% 226.6 / NA 74P132S (CCG→TCG) ugpCsn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/40);  minor base C (1/0);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCT  >  NC_000913/3588543‑3589010
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               
gCAACACCGCCGTTCGTCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAATGTAGAGTGAAGCCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCCGCTTGGCATCGAGGCTAGAGAGCGGCTCATCAAACAGGAACACAGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGCCCGCCGGAAAGCTCGCGCGAGCgccg                                                                                                                                                                                                                           >  1:45620/1‑248 (MQ=255)
gCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACg                                                                                                                                                                                                                           >  1:176690/1‑251 (MQ=255)
 cAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACATGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGAGCGGCCCATCGCCACACGCTGGCGATGACCGCCGGAAAGCTCGCGCGAGCGACGt                                                                                                                                                                                                                          >  1:45499/1‑251 (MQ=255)
  aaCACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACATGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCACATCGCCACACGCTGGCGATGACCGCAGGACAGCTCGCGCGAGCGACGtt                                                                                                                                                                                                                         >  1:210445/1‑251 (MQ=255)
    cacCGCCGTTCATCACCAGTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTg                                                                                                                                                                                                                       <  2:119952/251‑1 (MQ=255)
     acCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTg                                                                                                                                                                                                                       >  1:302247/1‑250 (MQ=255)
              catcgacATTACTCCCTGGGTGAGCGGCATCGCTGCACCCTGATCGTGATGAACGTAGAGTGAAGTCGGTTTCAGGCGACGATTCAACTGGTGCAGTTCAAGACACATCTGCACGCGCTGCTGGGCATCGAGGTTAGAGAGCGGCGCATCAAACAGGGACACCGCCGGTTCGCGCACAATCTCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGGAAGCTCTCGCGAGCGACGTTTTAGCTGACCg                                                                                                                                                                                                              <  2:203552/244‑1 (MQ=255)
                    cATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCt                                                                                                                                                                                                       <  1:7524/251‑1 (MQ=255)
                       tACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTcc                                                                                                                                                                                                     <  1:105405/250‑1 (MQ=255)
                               gggCGAGCGTCATCGCTTCAACCTGTTCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCTGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATg                                                                                                                                                                                               <  1:244309/248‑1 (MQ=255)
                                  ggAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgcc                                                                                                                                                                                         <  2:195656/250‑1 (MQ=255)
                                   gAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGCTCAAGACGCATCTGCACGCGCAGATTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATAGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGCGCGACGTTTGAGCCGACCGTCCCGCTCCAGAATGCGcgccg                                                                                                                                                                                        >  1:402824/1‑251 (MQ=255)
                                       gtccgcGCTTCATCCTGATCGGGAGTAACGTAGAGCGAAGTCGTTTTCAGGCGACGGTGCCACTGTTGCAGTTCAAGCCGCATCTGCCCGCGCCGCTTGGAACCGAGGTTAGAGGGCGGCTCATCAAACAGGACCTACGGCGGATCGCGCACAACCGCGCGGCACATCGCCTCACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGGCCAGCTCCAGAATGCGCGCCGcttc                                                                                                                                                                                    <  2:113954/246‑1 (MQ=255)
                                       gTCATCGCTTCAACCTGATCGGGAGGAACGAAGAGGGAGGTCGGTGTCAGGCGACGGTGCAACTGTTCCATTTCAAGCCGCATCTGCACGCGCAGTTATGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCAGCGCCACACGCTGGCGCTGACCGTCGGAAAGCTCGCTCGAGCGACGTTTGATCAGACCGTCCAGCTCCGGAATTCGCCGCGcttc                                                                                                                                                                                    <  2:28256/251‑1 (MQ=255)
                                            cGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTa                                                                                                                                                                                >  1:11094/1‑250 (MQ=255)
                                                tCAACCTGAGCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAcg                                                                                                                                                                             <  2:245505/249‑1 (MQ=255)
                                                    cctgaatgtgaatagcGTAGAGTGTAGTCTCTTGCAGGCGACGGTCCAATTGGTGCAGTTCAAGGCCAATCTGCACGCGCAGCTTGGCATGGAGGTTAGAGAGCGCTTCATCAAACAGGAACACCGCCGGATCGCGCTCAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCTCGAGCGACGTTTGAGCAGATCGGCCAGCTCCAGAATGCGCGCCGCTTCTTTACCGTGCTCg                                                                                                                                                                       <  2:85462/236‑1 (MQ=255)
                                                      tGATCGTGCGTCACGTAGCGTGAAGTCGTTTTCAGGCGACGGTGCAACTGCTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGCTCGCGCCCAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGc                                                                                                                                                                     >  1:344987/1‑251 (MQ=255)
                                                       gATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAACCAGGAACACCGCCGGATCGCGCACAAGCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGGCCAGCTCCAGAATGCGCGCCGCTGCTTTAACGCGCTCGGc                                                                                                                                                                     <  2:155714/250‑1 (MQ=255)
                                                       gATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCa                                                                                                                                                                    <  1:219417/251‑1 (MQ=255)
                                                       gATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTACAGAATGCGCGCCGCTTCTTTAACGCGCTCGGc                                                                                                                                                                     >  1:260339/1‑250 (MQ=255)
                                                          cgggagaaaCGTAGAGTGAAGGCGTTTTCAGGCGACGGTGCAATGGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCAGCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCGGGCGCTGACCGCCGTAAAGCTCGCGCGAGCGACGGTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAtt                                                                                                                                                                 <  2:205941/244‑1 (MQ=255)
                                                           gTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAttt                                                                                                                                                                <  1:225605/251‑1 (MQ=255)
                                                               gTAACGTAGATTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGGTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTtgctg                                                                                                                                                            <  1:403071/251‑1 (MQ=255)
                                                                 aaCGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTgctgct                                                                                                                                                          >  1:311525/1‑251 (MQ=255)
                                                                    gTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTCGGCATCGAGGTTAGAGAGCGCCTCATCAACCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGCCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGc                                                                                                                                                       >  1:185379/1‑251 (MQ=255)
                                                                           gAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCGCATCAAACAGGACCACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGcc                                                                                                                                                <  2:299127/251‑1 (MQ=255)
                                                                              gTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCcgcg                                                                                                                                             <  1:292366/251‑1 (MQ=255)
                                                                                 gTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAAt                                                                                                                                          >  1:266829/1‑251 (MQ=255)
                                                                                  ttttCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAAtt                                                                                                                                         >  1:366978/1‑251 (MQ=255)
                                                                                       aGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTc                                                                                                                                      >  1:122384/1‑249 (MQ=255)
                                                                                        ggCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGACTGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGc                                                                                                                                   >  1:16551/1‑251 (MQ=255)
                                                                                          cGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGccc                                                                                                                                 >  1:62349/1‑251 (MQ=255)
                                                                                             aggggCAACTGTTGAAGTTCAAGCCGCATCTGCACGCGCAGTTTGGCATCGAGGTTAGACAGCGGCTCTTCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCTGCTCCAGAATTCGCGCCGCTTCTTTAACGCGCTCGGCAAGTTGCTGCTTGCCCATGCCGCGAATTTTCAGccctc                                                                                                                               <  2:104252/246‑3 (MQ=255)
                                                                                               gTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACg                                                                                                                            <  1:69523/251‑1 (MQ=255)
                                                                                                    aCTGTTGCAGTGCAAGACGCATCTGCACGCGCAGCTTGGTATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCTCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATg                                                                                                                       <  1:203719/251‑1 (MQ=255)
                                                                                                           cAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGCTAGAGAGCGGCTCATCAAACAGGAACACCGCCGCAGCGCGCACACTCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGCCGTTTGAGCAGACCGTCCAGCTCCCGAATGCGCGCCGCTTCTTTCACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTAAGCCCCCACGCCACGTTttctt                                                                                                                >  2:378036/1‑251 (MQ=255)
                                                                                                            aGTTCAAGACGCATCTGCAAGCGCAGCTTGGCCTCGCGGTAAGAGAGCGGCTCCTCAAACAGGCACACCGCCGGATCGCGCACCATCGCGCGGCCCATCGACACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGCCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCATTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGAGCATTTTCAGCCCACACGCCATGTTttctt                                                                                                                >  2:234468/1‑250 (MQ=255)
                                                                                                             gTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCg                                                                                                              >  1:402992/1‑251 (MQ=255)
                                                                                                                 aaGACGCATCTGCACGCGAAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAAGCGCTCGGCAATTTGCGGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCACTGTTTTCTTCGaccc                                                                                                          >  2:193786/1‑249 (MQ=255)
                                                                                                                 aaGACGAATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATAGCGCGGCCCATCGCCACCCGCTGGCGCTGACCGCAGGAACGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCCGCCCCCACGCCATGTTTTCTTCTacac                                                                                                          >  1:44700/1‑251 (MQ=255)
                                                                                                                  aGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACt                                                                                                         <  1:195950/251‑1 (MQ=255)
                                                                                                                  aGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACt                                                                                                         <  1:359465/251‑1 (MQ=255)
                                                                                                                    aCGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCAATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTc                                                                                                        >  1:369815/1‑250 (MQ=255)
                                                                                                                    aCGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCACCAAACAGGAACACCGCCGGATCGCGCACAACCGCGCGGCCCATCGCCACACGCGGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCa                                                                                                       <  2:100482/251‑1 (MQ=255)
                                                                                                                       cATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCCCGCCATGTTTTCTTCGACACTCatat                                                                                                    >  2:296719/1‑251 (MQ=255)
                                                                                                                          cTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATAGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCg                                                                                                 >  1:207062/1‑251 (MQ=255)
                                                                                                                           ggCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCgg                                                                                                <  1:383302/250‑1 (MQ=255)
                                                                                                                                 cgcAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACAAGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCCATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATaa                                                                                            >  1:38943/1‑249 (MQ=255)
                                                                                                                                   cAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCTGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACTCTCATATGCGGATAAAgcg                                                                                        <  1:346186/251‑1 (MQ=255)
                                                                                                                                          gCATCGAGGTTGGAGAGCGGCTCATCAAACAGGAACGCCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCGGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATTCGGATAAAGCGCGTAGtt                                                                                 <  2:383914/251‑1 (MQ=255)
                                                                                                                                            aTCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCAGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGACTTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCt                                                                               >  2:253035/1‑251 (MQ=255)
                                                                                                                                                 ggTTAGAGAGCGGCTAATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGCTAAAGCGCGTAGTTCTGGAac                                                                          >  1:268927/1‑251 (MQ=255)
                                                                                                                                                    tAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACAcc                                                                       >  2:99841/1‑251 (MQ=255)
                                                                                                                                                                 atcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCcgc                                                          <  1:253035/251‑1 (MQ=255)
                                                                                                                                                                     aacgggAACCCCGCCGGATCGCGCACAAGCGCGCGGCCGATCGCCACACGCTGGCGCTGACCGACGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATc                                                      <  2:176690/247‑1 (MQ=255)
                                                                                                                                                                       cAGGAAAACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACCCGCAGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCtt                                                    >  2:193417/1‑251 (MQ=255)
                                                                                                                                                                              acCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTGGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTGCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgcgc                                                                                       <  1:81022/209‑1 (MQ=255)
                                                                                                                                                                               cctccgGATCGCGCACAACCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTATCGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCTCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTcc                                            <  2:194767/248‑1 (MQ=255)
                                                                                                                                                                                cgccgGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGCCCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGCTCTTTTGGCTccc                                           >  1:182856/1‑250 (MQ=255)
                                                                                                                                                                                 gccgGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAt                                          >  1:246735/1‑251 (MQ=255)
                                                                                                                                                                                   cgGATCGCGCACAAGCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAttt                                        <  2:122384/251‑1 (MQ=255)
                                                                                                                                                                                                   gcgcgGCCCATCGCCTCTCGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCAAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTGCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCATTCTCGCGCTGGTc                        <  2:311525/251‑1 (MQ=255)
                                                                                                                                                                                                   gcgcgGCCCATCGCCACACGCGGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGAGCTTTTGGCTCCATTTCAGTCACGCGCTGGTc                        <  1:296719/251‑1 (MQ=255)
                                                                                                                                                                                                      cgGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGtt                     >  2:83903/1‑251 (MQ=255)
                                                                                                                                                                                                       ggCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTTAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTg                    <  1:234468/251‑1 (MQ=255)
                                                                                                                                                                                                       ggCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCCATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAc                                 >  2:148/1‑238 (MQ=255)
                                                                                                                                                                                                       ggCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAc                                 <  1:148/238‑1 (MQ=255)
                                                                                                                                                                                                          ccttCGCCCCACGCTGGCGCTGCCCGCCTGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCCTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATc                 <  2:303263/248‑1 (MQ=255)
                                                                                                                                                                                                               cccccccGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTCGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGTATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATGTCAGTCACGCGCTGGTCGTTGATCCAGat            <  2:384009/245‑1 (MQ=255)
                                                                                                                                                                                                                  aaacGCTGGCCCTGACAGCCGGAAAGTTCGAAAGAGCGACGTCCGACCAGACCGACCAGCTCCGGATTCCACGCCGCTCCTTTCACGAGCTCGGCAATTTGCTGCTTTACCATGCAGCGACTTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCCTATGCGGCTCAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGTTCTTTTGGCTCCATTTCAGTCACACGCTGGCCGTCGAtaccaatatc         >  2:147276/3‑242 (MQ=255)
                                                                                                                                                                                                                     cGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGcc      <  2:372474/251‑1 (MQ=255)
                                                                                                                                                                                                                         ggCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt  <  1:321830/251‑1 (MQ=255)
                                                                                                                                                                                                                         ggCGCTGACCGCCGGAAAGCGCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGTGAATTTTCAGCCCCCACGCCAGGTTGTCTTCGACAGTCATATGCGGATGAAGTGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTGTGGGCGCCAGTGCAGGCACGCGGTGGTCGTTGATCCAGATATCGCCTTCt  <  2:344987/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               
GCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCT  >  NC_000913/3588543‑3589010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑