Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,585,517	G→A	A436V (GCC→GTC)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,585,517	0	G	A	98.0%	162.4 / NA	50	A436V (GCC→GTC)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/28);  minor base C (1/0);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CTGATGGTGGAAACGCGGCGCATTGGTC‑GCTTCGGCGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATAGGCTTTATTGGT  >  NC_000913/3585273‑3585761                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cggATGGTGAAAAGGCGGCGC‑TTGGTCCGCTTCGGGGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGCCGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGgaga                                                                                                                                                                                                                                                <  2:185182/249‑1 (MQ=255)          gAAACGCGGCGCATTGGTC‑GCTTCGGCGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATcatcc                                                                                                                                                                                                                                        >  1:382315/1‑250 (MQ=255)                  gctcagtGGTC‑GCTGCGGCGACTGTCAAGCCATAATCGATGCTATTCACCACCATTTTCAGCACGGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCTGTTTGACGGAGAAATCAGCCATCTGGtta                                                                                                                                                                                                                               <  2:338363/245‑1 (MQ=255)                  gcgcATTGGTC‑GCTTCGGCGACGTTCAAGCCATAATCGATGCTATTAACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGAGGGCGATTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGtt                                                                                                                                                                                                                                >  1:190743/1‑250 (MQ=255)                    gcATTGGTC‑GCTTCGGCGACTTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatt                                                                                                                                                                                                                             <  1:312323/251‑1 (MQ=255)                    gcATTGGTC‑GCTTCGGCGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGCCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatt                                                                                                                                                                                                                             >  2:116405/1‑251 (MQ=255)                       ttGGTC‑GCTTCGCAGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGCCGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTaa                                                                                                                                                                                                                           <  2:279158/250‑1 (MQ=255)                                  cgtcGACGTTCAAGCCATAATCGATGCTATTCACCACTATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCCCCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACTTTCGGTACGCCCGTTTTGACGGAGAAATCATCCATCTGGTTAGTAAGCGGAATACCg                                                                                                                                                                                                                <  1:304341/248‑1 (MQ=255)                                              aGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCcgcg                                                                                                                                                                                                    <  2:315429/251‑1 (MQ=255)                                                    aaTCGCTGCTATTCACCACCATTTGAAGCAATGTAGTGATGATCAGGCTACCGACTGGGCTACCGGTCAACAGACAGGTTTTAACGTATTTGACCGCAATGGTCGGCGCCATCGAAGACAGCGGGCGTTTGTTCGGCCAGACGGCGTTGGCCTCACCGACCCAC‑AGCACGTAATCGTTCGGTACGGAAGGTTTGACGGAGACATCATCCATCTGGTTATTAAGCAGAATACCGCCCTCGCCCGCGACattg                                                                                                                                                                                              >  2:397171/1‑248 (MQ=255)                                                       cGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTTCCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGGATACCGCTCTCGCCCGCGACAATGccc                                                                                                                                                                                           <  2:364573/251‑1 (MQ=255)                                                       cGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGCCAATGccc                                                                                                                                                                                           >  2:124169/1‑251 (MQ=255)                                                        gATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCg                                                                                                                                                                                          <  1:94912/251‑1 (MQ=255)                                                        gATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCg                                                                                                                                                                                          >  1:101300/1‑251 (MQ=255)                                                        gATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCg                                                                                                                                                                                          >  2:29229/1‑251 (MQ=255)                                                         aTGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAAGCAGCCAGGTTTTACCGTCTTACGCCACAAGGGTCGGCGACAGCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGGTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGt                                                                                                                                                                                         <  1:327559/251‑1 (MQ=255)                                                         aTGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGACTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGt                                                                                                                                                                                         <  1:105825/251‑1 (MQ=255)                                                                                        tgatgaTCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGTCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATcatccagct                                                                                                                                                                                                                                    <  2:384777/177‑4 (MQ=255)                                                                                        tgatgaTCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCt                                                                                                                                                                                                                                    >  1:384777/1‑177 (MQ=255)                                                                                                   cTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATTCCCGTACCGGAGGGGGTGTTCAGCGGATAGGTCACCGCCACCGCGtt                                                                                                                                               >  2:111422/1‑251 (MQ=255)                                                                                                            gggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgccacc                                                                                                                                                    <  1:290942/237‑1 (MQ=255)                                                                                                            gggCTACCGGTAACCAGCCAGGTTTTAACGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgccacc                                                                                                                                                    >  2:290942/1‑237 (MQ=255)                                                                                                            gggCGACCGGTAAACAGACAGGTTTTACCGTCTTTCACCACAAGTGTCGGCGACATCCCCGCCTGCGGGCGTTTTTTCGGCCCGACGGCGTTGGCATCACCG‑CCCCCCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCACCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCCCCGCGTTACCATCt                                                                                                                                        <  2:146759/249‑1 (MQ=255)                                                                                                             ggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACCATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTa                                                                                                                                              >  2:117172/1‑242 (MQ=255)                                                                                                             ggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGGGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTa                                                                                                                                              <  1:117172/242‑1 (MQ=255)                                                                                                                           agccagGTTTTACCGTATTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGATTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCCCCGCCACCGCGTCACCAACTTTATCCACCACTGAGt                                                                                                                        >  2:249125/1‑250 (MQ=255)                                                                                                                                     tACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGtt                                                                                                                                               >  1:345617/1‑217 (MQ=255)                                                                                                                                     tACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGtt                                                                                                                                               <  2:345617/217‑1 (MQ=255)                                                                                                                                      aCCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGttt                                                                                                            <  1:388296/251‑1 (MQ=255)                                                                                                                                              tCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCTACGGCGTTGGCATCACCG‑CCCACAAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTAct                                                                                                     <  2:84877/250‑1 (MQ=255)                                                                                                                                              tCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTAct                                                                                                     <  1:323943/250‑1 (MQ=255)                                                                                                                                                     aaTGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCCTCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGAGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATa                                                                                               >  1:357955/1‑249 (MQ=255)                                                                                                                                                     aaTGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGCTTACTCTCATa                                                                                               >  1:205224/1‑249 (MQ=255)                                                                                                                                                           cGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGCCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGAGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGCGTAATGGGTAGTTTGATTACTCTCATAAGGCGCa                                                                                        >  1:403234/1‑250 (MQ=255)                                                                                                                                                                aCATCGCCGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCAACCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTg                                                                                  <  1:309546/251‑1 (MQ=255)                                                                                                                                                                aCATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGCTTACTCTCATAAGGCGCAAGCTTg                                                                                  >  2:352646/1‑251 (MQ=255)                                                                                                                                                                  aTCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGcc                                                                                <  1:225664/251‑1 (MQ=255)                                                                                                                                                                    cgccgacAGCGGGCGTGTGTTTGGCCCGACGGCGTTGGCATCACCG‑TCCTCCAGCACGTAAACGGTCGGTACGACCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGTGTATAGTTCACCGCCACCGCGTTACCATCTTGATCATCCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCTAGCTAGCCgg                                                                              <  2:371291/248‑1 (MQ=255)                                                                                                                                                                                  gTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGGGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTaa                                                                                                                      <  2:144649/197‑1 (MQ=255)                                                                                                                                                                                  gTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGGGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTaa                                                                                                                      >  1:144649/1‑197 (MQ=255)                                                                                                                                                                                  gTTTGTTCGGCCCGACGGCGTTGGCATCAACG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAAGCATCCAGCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGTTGGTGTTCAGCGGAGAGGTCACCGCCGTCGCTTTGCCATCTTTATCCCCCGCTGAGTAATGGGTATTTTGATTACTCTCATAAGGCGCATGCTTGCCGGGGCGAATTTCGCTg                                                                <  2:361024/251‑1 (MQ=255)                                                                                                                                                                                   tttGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTCACCGTTCGGTAAGCTCGGTTTGACGGAGCAATCATCCATCTGGGTATTAAGCAGAATACCGCTCTCGCACGCGACAATGCCCGTACCGAAGGCGGTGTTCAGCGTACAGGTCACCGCCACCGCGTTACCATCTTTATCAACCACTGAGTAATGGGTAGTTTGATTACTCTCAAAAGGCGCACGCTTGCCGGCGCGACTTTCGCTgg                                                               >  2:100901/1‑251 (MQ=255)                                                                                                                                                                                          ggCCCGGCGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTCACGAAGGTGGTGTTCAGCGTATAGGTCACCGCCTCCGCGTTGCCATCTTTATCCACCACAGAGTAATGGGTAGTTTGAGTACTCTCATAAGGCGCAATCCTGCCGGGGCGAGTGTCGCTGGATGGCtt                                                        <  2:69272/251‑1 (MQ=255)                                                                                                                                                                                              cGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGATAAATCATCCATCTGGTTAATAAGCAGAATACCGCGCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTGCAGCGTATAGGTCGCCGCCACCGCGTTACCATCTTTATCCACCGCTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCGTCGCt                                                    <  2:357955/251‑1 (MQ=255)                                                                                                                                                                                                      ttGGCATCACCG‑CACACCAGCCCGTAAACGTTCGATACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGat                                            >  1:67823/1‑251 (MQ=255)                                                                                                                                                                                                                              gTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGAtt                     >  1:77304/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaCGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTgg                  <  1:285111/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATGCCGCTCTCGCCCGCGACAATGCCCGGACCTAAGGTGGGGTGCAGGGTATAGGTCACCGCCACCGCGTTACCATCTTTAGCCACCACTGAGTAATGGGTAGTGTGATTACTCTCATAAGGCGCAATCTTGCCGGGGCGAATTTCGCTGGAGGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTGGGCATAg             <  1:397694/250‑1 (MQ=255)                                                                                                                                                                                                                                                cGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCACGCACCGACGGTGGTGTTCAGCGAATAGGTCACCGCCACAGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGAATACTGTCATAAGGCGAAAGCTTGCCGGGGCGAATTTCGCTGGATGGATTCGCTTTATTGATATCAATTTGATAGGCAATAGCTTTGGCAGAGGCTTTATTgg   >  1:179916/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ggTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATAGGCTTTATTGGt  <  2:101300/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CTGATGGTGGAAACGCGGCGCATTGGTC‑GCTTCGGCGACGTTCAAGCCATAATCGATGCTATTCACCACCATTTGCAGCACTGTAGTGATGATCCGGCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCG‑CCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATAGGCTTTATTGGT  >  NC_000913/3585273‑3585761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑