Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,583,969	G→A	W98* (TGG→TAG)	insB6 →	IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,583,969	0	G	A	88.9%	16.0 / ‑3.2	9	W98* (TGG→TAG)	insB6	IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (3/5);  total (3/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTT  >  NC_000913/3583946‑3584215                        |                                                                                                                                                                                                                                                       gTCACACTTTGACGTGGAGCAATAGATGACGGATGGCTGGCCGCTGTATGACTCCCGCCTGAAGGGAAAGCTGCACGTAATAAGCAAGCGATATACGCAGCGACTTGAGCGGCATACCCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCACAATCGGTGGAGCTGCATGACAAAGTCATCGGGCCTTATCTGAACATACAACACTATCAATAAGTTGGAGTCATTACCg                     >  2:238566/1‑251 (MQ=11)  tCACCCTTTGACGTGGTGATATGGATGACGGATTGCTTGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGTCAAAGTCATCGGTCATTATCTGAACATAAAACACTATCAATAAGTTTGAGTCATTACCg                     <  1:350615/250‑1 (MQ=2)      ccTTTGACGTGGTGATATAGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAACTCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACCTAAAACAATATCAATAAGTTGGGGTCATTCCCGACAtt                >  1:391118/1‑251 (MQ=255)             cGTGGTGATATAGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTa         <  1:198836/251‑1 (MQ=25)              gtggtgATATAGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTaa        <  1:182232/251‑1 (MQ=25)              gtggtgATATAGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTa         >  1:130575/1‑250 (MQ=25)                 gtgATATAGAGGACGGATGGCTGTCCGCTGTATGAATCCCGCCTGAAGTGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCTCCTGGTACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTTGAGCTGCATGAAAAAGTCATCGGGCATTATCTGAACATAAAACTCGAGCAATAAGTTTGAGGCATGACCAACATTAAAAGTAACtt     <  2:188432/251‑1 (MQ=11)                  ggATATAGATGACGGATGGCTGGCCGCTGTCTGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACttt    <  1:278459/250‑1 (MQ=255)                    atatAGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACttttt  <  1:234223/251‑1 (MQ=38)                        |                                                                                                                                                                                                                                                       GTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTT  >  NC_000913/3583946‑3584215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑