Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,119	A→G	intergenic (+253/+503)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,119	0	A	G	100.0%	142.1 / NA	55	intergenic (+253/+503)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

CAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGC  >  NC_000913/3559881‑3560363                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      cAGCGCCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATa                                                                                                                                                                                                                                            <  1:274803/250‑1 (MQ=255)      ccTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATTCCGAAATCAAGGCGGGACTGGGAGATaaagact                                                                                                                                                                                                                                      >  1:159955/1‑247 (MQ=255)                gCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGCAATCCAGGCGGGACTGGGAGATAAAATCGAGCGTCGCtt                                                                                                                                                                                                                            >  1:170200/1‑251 (MQ=255)                gCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCtt                                                                                                                                                                                                                            <  1:125013/251‑1 (MQ=255)                       ggcggcGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCa                                                                                                                                                                                                                       <  1:139826/249‑1 (MQ=255)                             tgggCGTCGGTGTCGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGTGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGAAAAAATCGACCGGCGCTTCTCCaaagctgcc                                                                                                                                                                                                               >  2:311574/2‑244 (MQ=255)                                        gtAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGc                                                                                                                                                                                                    <  1:232875/251‑1 (MQ=255)                                          aGACGGCATCTACCATGCTGATGCGGACCATATTGACCAACGCCTTGCGGCAAAATTTCGAGTGAGCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGGGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAAGGATCCCGCCATCGCGGCt                                                                                                                                                                                                   <  1:222481/250‑1 (MQ=255)                                              ggCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGCGCGTTGCTTCGCCAATGATCCCGCCATCGCGGCTGCGt                                                                                                                                                                                               >  1:403498/1‑250 (MQ=255)                                               gCATCTACCATTCTGATGCTGTCCATATGGTCCGTCGCGTTTCGTCCAAATTGCGAGTGATCCACTACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACGTCGTGTTAATCGAACTCCAGCAGCGAGCCTTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCTTGCGTCGCTTCGCCAAGGATTCCGCCATCGCGGCTGCGTaa                                                                                                                                                                                             <  2:122712/251‑1 (MQ=255)                                                     aCCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAAGGGCGCGTTTGGTGCGAACTTCGGGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTc                                                                                                                                                                                          <  2:336661/248‑1 (MQ=255)                                                     aCCAGGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACGCCAGCAGCGAGCCGTCGCTAGCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTc                                                                                                                                                                                          <  2:202091/248‑1 (MQ=255)                                                       cATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCAc                                                                                                                                                                                     <  1:366445/251‑1 (MQ=255)                                                        atgctgatgctgCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCAcc                                                                                                                                                                                    <  1:147654/251‑1 (MQ=255)                                                                gctccccgtactgtccactgcgTAACGGGCAAATTTCGAGTGATCGACAACCAGCAGAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTGCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACGGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGaa                                                                                                                                                                            <  2:13899/232‑1 (MQ=255)                                                                  tgcCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCCTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAatg                                                                                                                                                                          >  2:287216/1‑251 (MQ=255)                                                                           ttaCCATCGCGTTACTGCCAAATTTCGAGTGATCGACAACCAGCACAACGTGGCGCGAGTTCTCAATAATGGCGCGGTTGGTGCGAACTTCGGGGTAATCGATTACCAGCAGCGAGCCGGCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAAACAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCGACGCCAATTATCCCGCCATCGCGGCTGCGTAATTCGCCGCCGGCGAGAATGATGCGata                                                                                                                                                                  <  2:78492/248‑3 (MQ=255)                                                                                 ccgcgTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGAGAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCTAAAAtcttct                                                                                                                                                           <  2:385412/250‑1 (MQ=255)                                                                                  cgcgTTACGGCCAAATTTCGAGTGAGCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATcttctt                                                                                                                                                          <  2:153174/251‑1 (MQ=255)                                                                                      ttgcggtcAAATGTCTAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGTGCGATTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGTGAGCCGTCGCTATCGATGCCGCGTCTCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTTTTTT‑a                                                                                                                                                      <  2:92949/243‑1 (MQ=255)                                                                                             cAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTAGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGAATGGGAGATAAAACCGAGCGGCGCCTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCAACGGCGAGAATGAAGGGAAAATCTTCTTTT‑ACCATccac                                                                                                                                              >  1:221772/1‑248 (MQ=255)                                                                                              aaaTTGCGAGTGATCGACAACCACCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCTCTAGCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGCCTGGGAGATTAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGTCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACg                                                                                                                                             <  2:198739/251‑1 (MQ=255)                                                                                                     gAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCCGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCTCCCCCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCCACGTGTTAGc                                                                                                                                      >  1:66960/1‑251 (MQ=255)                                                                                                         gATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAAc                                                                                                                                   <  1:352041/250‑1 (MQ=255)                                                                                                          aTCGACAACCAGCATACCGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGGGATAAAATCGAGCGTAGCTTCGCCAATGAACCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGAAGCGAAAATCTTTTTTT‑ACCCTCAACGTGTTAGCAACGt                                                                                                                                 >  2:189892/1‑251 (MQ=255)                                                                                                            cGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGACCTCCAGCAGCGAGCCGTCGCTATCGATCCCGCCTATCCCCAGACTGCCGAACTCACAGCGGGAATGGGAGATAAAATCGACCGTACCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGACACCGGCGAGAATGATGCGCAAATCTTCTTTT‑CCCATCCCCGCGTTAGCAACGTTg                                                                                                                               >  1:171282/1‑251 (MQ=255)                                                                                                             gACAACCAGCATAACGTGGCGCGCGTTCTCAATAATGGCGCGTTTGGTGAGCACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTCTCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGCCTGGGAGATAAAACCCAGCGTCGCTTCGCCCATGATCCCGCCATCGCGGCAGCGTAACTCGCCACCGGCGGGAAATATGGGGAAAACTTCTTTT‑ACCATCAACGTGGTTGCAACggtt                                                                                                                               >  2:364176/1‑247 (MQ=255)                                                                                                              aCAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACGCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACGGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTgag                                                                                                                             <  1:270724/251‑1 (MQ=255)                                                                                                                 aCCAGCATAACGTGGCGCGAGTTCACAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCGTCTTTT‑ACCATCAACGTGTTAGCAACGTGGAGAtt                                                                                                                          <  2:61687/251‑1 (MQ=255)                                                                                                                   cAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGTGACGGGGAGATAAAATCGAGCGTCGCTTCGCCAAGGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGAttgt                                                                                                                        <  1:242640/251‑1 (MQ=255)                                                                                                                   cAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGAttgt                                                                                                                        <  1:164329/251‑1 (MQ=255)                                                                                                                    aGCATAACGTGGCGCGAGTTCTCAATAATGTCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGAttgtt                                                                                                                       <  1:328904/251‑1 (MQ=255)                                                                                                                              ggCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATg                                                                                                             <  1:113700/251‑1 (MQ=255)                                                                                                                                cgcgAGCTCTAAATAATGGAGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAAACAAGGCGGGACAGGGGGATAAAATCGAGGGTCGCTTAGCCAAAGATTCCGCCATCGCGGCTGCGTGATTCTCCAACGGCGAGAAAGATGCGAAAACATTATTTT‑AACTTCAACGTGGTTGTAACGTTggtgatgttggtgataaattg                                                                                                           >  2:4520/1‑231 (MQ=255)                                                                                                                                  cgAGTTCTCAATAAGGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACCGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCa                                                                                                         <  1:8497/251‑1 (MQ=255)                                                                                                                                     gTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGGGGGAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGGGCGTCGGTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCGAAAGATGCGAAAAACTTCTTTTTACAAT‑AAAGTGTTAGCACCGTTGAGGTTGGTGGTTACAATgcaccaat                                                                                                      >  2:300570/1‑245 (MQ=255)                                                                                                                                       tctcAATAATGGCGCGTTTGGTGAGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCAGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATTGAGCGTCGCTTCGCCAATGATCCCGCCCTCGCGGCTGCGTAATTCGCCACCGGGGAGAATGATGCGGAAATCTTCTTTT‑ACCCTAAAAGTGTTAACAACGTTGAGGTTGTTGGTGACAATACGCAAATTg                                                                                                    >  2:18848/1‑251 (MQ=255)                                                                                                                                        ctcAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGGCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGAAAATCTTCTTTT‑ACCATCAACGCGTTTGCAACGTTTAGGTTGTTGGGGACAATGAGCAAATTgt                                                                                                   >  2:294167/1‑250 (MQ=255)                                                                                                                                        cgcAATAATGGCGTGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTg                                                                                                    <  2:303168/248‑1 (MQ=255)                                                                                                                                                  gcgcGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGGGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAACTCTTCTTTT‑ACCATCCACGAGTTAGCAACGTTGAGGGTGTTGGGGGCAATGCGCAAGTTGCTGTGATTg                                                                                           >  1:345823/1‑249 (MQ=255)                                                                                                                                                   cgcgTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGCAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGc                                                                                        >  2:348400/1‑251 (MQ=255)                                                                                                                                                      gTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAgg                                                                                     >  1:192314/1‑250 (MQ=255)                                                                                                                                                                aaCTTCCTGGTAATCGAACGCCAGCATCTATCCGTCGCTATCGATGCCGCTTATCCCCATAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCAGCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTa                                                                           <  2:233280/251‑1 (MQ=255)                                                                                                                                                                              cgaccTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCGTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTcc                                                                   <  2:17339/241‑1 (MQ=255)                                                                                                                                                                              cGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTcc                                                                   >  1:17339/1‑245 (MQ=255)                                                                                                                                                                                    ccAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATGGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCg                                                       <  2:73968/251‑1 (MQ=255)                                                                                                                                                                                            gAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAAAGTGTTAGCAACGTTGAGATTGTTGGTGAAAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAACGCTTCCGGCGTGGTGCCGATATCGa                                                >  1:351427/1‑250 (MQ=255)                                                                                                                                                                                             aGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGAt                                               >  1:254369/1‑250 (MQ=255)                                                                                                                                                                                                  tCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAAc                                           >  1:350036/1‑249 (MQ=255)                                                                                                                                                                                                                  ttATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGAtt                                                                                            <  1:72350/184‑1 (MQ=255)                                                                                                                                                                                                                      ccccAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGAtt                      <  1:3676/250‑1 (MQ=255)                                                                                                                                                                                                                         cAGAATGCCGAAATCAAGGCGGGACTGTGAGAGAAAACCGCGCGGCCCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTATCGCGTCCGGCGTGGTGACGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCt                  <  2:12957/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTAGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGGTTGTTGGTGACAATGCGCAAATTGCTGGGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACttt        >  1:27495/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAAGGCGGGACTGGGAGATAAAAGCGAGCATAGCTTCGCTAATGATCCCGCCATCGCGGTTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑TCCTTCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTCCGCTACTGCTTCCGGCGGGGCTCCGATATCGATAAACAGCTTCGGCCCATTGGGTATTTGCTCCGCCACTTTGCg     <  2:177189/250‑1 (MQ=255)                                                                                                                                                                                                                                         aGGCGGGACTGGGATATAAAACCGAGCGTCTCTTCGCCAATGACCCCGCCATCGCGGCGGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGGGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGTTGCCGATATCGTTAAACAGCGTCGAGCTATTGGGGATTTGCTCCGCCACTTTGCGGGc  <  2:345823/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTT‑ACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGC  >  NC_000913/3559881‑3560363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑