Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,647	T→C	I391V (ATT→GTT)	mtr ←	tryptophan:H(+) symporter Mtr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,647	0	T	C	100.0%	162.6 / NA	48	I391V (ATT→GTT)	mtr	tryptophan:H(+) symporter Mtr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/29);  total (19/29)

TCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTG  >  NC_000913/3304411‑3304889                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    tCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCg                                                                                                                                                                                                                                       >  1:259135/1‑251 (MQ=255)       gAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGc                                                                                                                                                                                                                                 <  1:118377/251‑1 (MQ=255)        aGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGcc                                                                                                                                                                                                                                <  2:149258/251‑1 (MQ=255)                  ggatgTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGAc                                                                                                                                                                                                                       <  2:232382/250‑1 (MQ=255)                    atgTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGAcgcg                                                                                                                                                                                                                    >  1:321685/1‑251 (MQ=255)                     tgTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCgg                                                                                                                                                                                                                   >  1:396396/1‑251 (MQ=255)                                 gttgttGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTg                                                                                                                                                                                                      <  1:294681/251‑1 (MQ=255)                                        ggATAGTGTGGCTTAACGCGGGAAGCCTTCTCCGAGCTGGCAACGATGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGCCGCGGAATTTCGGG‑CTGCCAAAGc                                                                                                                                                                                               >  2:360459/1‑251 (MQ=255)                                         gATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCg                                                                                                                                                                                              >  1:78668/1‑251 (MQ=255)                                                 gtcgtAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCATTAAATTAAAGCTCGATAAAAGATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTAcgcg                                                                                                                                                                                      <  1:261019/247‑1 (MQ=255)                                                                    tATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAgc                                                                                                                                                                     <  2:321685/249‑1 (MQ=255)                                                                       ccGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCgg                                                                                                                                                                >  2:86735/1‑251 (MQ=255)                                                                         gAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGaa                                                                                                                                                              <  1:106368/251‑1 (MQ=255)                                                                           gCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAAGTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACa                                                                                                                                                            <  2:61249/251‑1 (MQ=255)                                                                                   cGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGGAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTgccgc                                                                                                                                                    <  2:41327/251‑1 (MQ=255)                                                                                       gTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGccc                                                                                                                                                  <  2:45767/249‑1 (MQ=255)                                                                                            acagaGACCTGATAAGCGAAGCGCTTCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATgg                                                                                                                                            <  2:315831/246‑1 (MQ=255)                                                                                               tAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGc                                                                                                                                        >  2:32429/1‑251 (MQ=255)                                                                                                  aCCTGATAAGCGAAGCTCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGc                                                                                                                                     <  1:245203/251‑1 (MQ=255)                                                                                                   ccTGATAAGCGAAGCGCATCAGGCATTGTGAAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACACGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCt                                                                                                                                    >  1:320158/1‑251 (MQ=255)                                                                                                      gATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATGTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTaaa                                                                                                                                 <  2:73640/251‑1 (MQ=255)                                                                                                          agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAg                                                                                                                             <  1:350967/251‑1 (MQ=255)                                                                                                             gaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCAt                                                                                                                          >  1:309927/1‑251 (MQ=255)                                                                                                                 cgcATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCCCCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAcc                                                                                                                      <  1:226289/251‑1 (MQ=255)                                                                                                                 cgcATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGAACAGCTGATTACTGATACACCGGAAGTCAATTAAAGCTAGATACAATATGCACCAGTGCGTTGCCGACGACCAACACCAGAACCAGCACAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCCGATGGTAGCCGCTAAACCAGCATAAcc                                                                                                                      >  1:231766/1‑251 (MQ=255)                                                                                                                             tgtgTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACa                                                                                                           <  2:130481/250‑1 (MQ=255)                                                                                                                                gtAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGa                                                                                                        <  1:23424/250‑1 (MQ=255)                                                                                                                                                tctgATAAGGCACCGCTGATTACTGATACAGCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACCCCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGTGGAATTTCGGG‑CGGCCAAATCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATTGCGTACAGGAATCCGTTCGGTaacaa                                                                                        <  1:404339/247‑1 (MQ=255)                                                                                                                                                   gATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGc                                                                                    <  1:78387/251‑1 (MQ=255)                                                                                                                                                        ggCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACACGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                               <  2:320158/251‑1 (MQ=255)                                                                                                                                                           aCCGCTGATTACTGATACACCGGCCGTCAATTAAAGCTCGATAACATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGAGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAAAAACAGCCCCCCcac                                                                            >  2:401517/1‑251 (MQ=255)                                                                                                                                                                           ccacaggcatgaaaTTAAAGCTCGATAAAAAATGCTCCATTGCGTTGCCGACGCCAAACACCATAACCCTGGCAATCATCGGGTTGCCACCCCAGACGCGGAATTCCGGG‑TTGACAAAGTGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGTCGCTAAACCAGCATAACCAATGGCGTCCAGGAATCCGTTAGGGAACAACAGCCCCCTCTCAACGGTCGGGGCAAAg                                                            <  2:290103/240‑1 (MQ=255)                                                                                                                                                                           acacCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACACCTGGCGGGGCaa                                                              >  1:253430/1‑249 (MQ=255)                                                                                                                                                                                ggCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGCAGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTAAGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCACCATAACCAATGGCGTACAGGAATCCGTTCGGGACCAACAGCCCCCCCACAACTGGCGGGGCAAAGGTc                                                         >  2:274489/1‑249 (MQ=255)                                                                                                                                                                                ggCAGTAAATTAAAGCTCGATAAAATATGCACCAGGGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAACCCAGCATAACCAAGGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAg                                                       <  2:196655/251‑1 (MQ=255)                                                                                                                                                                                     tAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATg                                                  >  1:212791/1‑251 (MQ=255)                                                                                                                                                                                      aaaTTAAAGCTCGATAAAATATGCACCAGTGCGGTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATg                                                  <  1:95700/250‑1 (MQ=255)                                                                                                                                                                                          tAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTGGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGTCGGGGCAAAGGTCAGCAATGCCGt                                              <  2:86467/250‑1 (MQ=255)                                                                                                                                                                                             aGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCAACGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAAGTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGTAACTACATCCCCCCCACAACTGGAGGTGCAAAGGTCAGCAATGCCTTTTTc                                          <  2:249038/251‑1 (MQ=255)                                                                                                                                                                                                       aaTATGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGccc                                 >  2:321921/1‑250 (MQ=255)                                                                                                                                                                                                        atatGCACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                               >  2:119819/1‑251 (MQ=255)                                                                                                                                                                                                             cACCAGTGCGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCCCCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCACCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                          <  1:160757/251‑1 (MQ=255)                                                                                                                                                                                                                   tGCGTGGCCGACGCCAAACACCAGAACTAGCGCAATCATCGGCTTGCCACCCCAGCCGCGGAATTTCGGG‑CTGCCAAAGAGATTACGCGATGCACGGGCTACCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGACTCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGACATCAATGCCGTTTTCACTAGGCCCACAGCCGAgtcgc                    >  2:2806/1‑250 (MQ=255)                                                                                                                                                                                                                     cGTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                  >  1:250792/1‑251 (MQ=255)                                                                                                                                                                                                                      gTTGCCGACGCCAAACACCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGa                 <  1:120039/251‑1 (MQ=255)                                                                                                                                                                                                                          cagACGCCAAACACCAAAACCAGCGAAATAAAAGCCTTGCCACCCCAGACGCGGAATTCCGGG‑CTGCCAAAGGGTTTACTCGAGGCACGGGCTAACAGAGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAAAAACAGCCCCCCCACAACGGGCGGGGCAAAGGTCAGCAATGCAGTTTTCAAGCGGCCCCCATCCGAGCCGTCGAAAcc             <  2:210874/249‑1 (MQ=255)                                                                                                                                                                                                                              cGCCAAACACTAGATCCAGCGCAATTCTCGGCTTGCCACCCCAGACGCGGAATTTCGGGTCT‑CCAAAGCGTTTACGCGATGCACGGGCTAACAGCGTCGGAACAATTGCCGCCCAGATGGAAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCTGGAACACTAGCCCCCCCTCAACTGGCGGGGCAAAGGCCAGCAATGCCGTGTTCAAGCGGGCCACATCCGTGTCGTCGAAGCCAAAc         <  2:372065/251‑1 (MQ=255)                                                                                                                                                                                                                                     cacCAGAACCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGACTTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGGCAGCAATGCCGTTTTCAAACGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTg  >  2:232638/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGACGCCAAACACCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGG‑CTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTG  >  NC_000913/3304411‑3304889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑