Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,292,312	C→T	P324P (CCC→CCT)	yraK →	putative fimbrial adhesin YraK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,292,312	0	C	T	100.0%	90.5 / NA	28	P324P (CCC→CCT)	yraK	putative fimbrial adhesin YraK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

GCCAGACGGTTTTACTCCAGTAGACTTTGATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGG  >  NC_000913/3292084‑3292539                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    gccaGACGGTTTTACTCCAGTAGACTTTGATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCa                                                                                                                                                                                                               <  1:29862/251‑1 (MQ=255)    aGACGGTTTTACTCCAGTAGACTTTGATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCg                                                                                                                                                                                                            <  2:103613/251‑1 (MQ=255)                       gACTTTGATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGcc                                                                                                                                                                                         <  1:109939/251‑1 (MQ=255)                             gATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGGTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTGCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTaa                                                                                                                                                                                   <  1:251015/251‑1 (MQ=255)                                     ttATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGAAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCg                                                                                                                                                                           >  2:361754/1‑251 (MQ=255)                                      tATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCgg                                                                                                                                                                          >  1:341128/1‑251 (MQ=255)                                                           aaGATTAAACACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGGGGTGAGCTGGAAACAGGaa                                                                                                                                                      >  2:112587/1‑250 (MQ=255)                                                                    aaCTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAAGATTCCTTTTCAGAACGGTATCCTGCCTGTTGATCCTTCCGGGCATGGCACCGTCAAGATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATGTCAgtg                                                                                                                                            <  2:99621/251‑3 (MQ=255)                                                                             cAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGccac                                                                                                                                   >  1:293807/1‑251 (MQ=255)                                                                                     cATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCg                                                                                                                           <  1:209324/251‑1 (MQ=255)                                                                                                       ggTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGCCAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGCTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGACATCGTGAGGTaaa                                                                                                            >  1:26380/1‑248 (MQ=255)                                                                                                                     aCAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGACTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGtttt                                                                                           >  1:83682/1‑251 (MQ=255)                                                                                                                             gTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGCATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGCATCATTCCTGTTGATCCTTCCGGGCATGGCACCGTCACTATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGCGAGCTGGAAACCGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTaacg                                                                                   >  1:25910/1‑251 (MQ=255)                                                                                                                                   aGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTaacgcaacgc                                                                             >  2:135756/1‑251 (MQ=255)                                                                                                                                    ggCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAAGCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCTCCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCt                                                                            <  2:79176/251‑1 (MQ=255)                                                                                                                                       gaagaaGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGAGTAACGCAACGCTTCg                                                                         <  2:43183/251‑1 (MQ=255)                                                                                                                                           aagTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCAc                                                                     <  1:288168/251‑1 (MQ=255)                                                                                                                                                cAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTa                                                                <  1:361754/251‑1 (MQ=255)                                                                                                                                                          gCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCGTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTTGCCAGTTAATCTTGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCTACTCTTCGGTACTGTTACCCctgctg                                                       <  2:251581/250‑1 (MQ=255)                                                                                                                                                                    ggTATTCGTATTGAAAATATCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGAGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGCAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAACAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACACCTGATGCTCCAGCGCAt                                            >  2:373116/1‑251 (MQ=255)                                                                                                                                                                                             gcggAGTTGCAAATATTCCTTTTCAGAACGGTATTCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAAGCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCAc                   <  1:12922/251‑1 (MQ=255)                                                                                                                                                                                                   ttGCAAATATTCATTTTCAGAACGGTATACTTACTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCAATGTTACCCCTGCGGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCgcg             >  1:194850/1‑249 (MQ=255)                                                                                                                                                                                                     gCAAATATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGa            >  2:109501/1‑250 (MQ=255)                                                                                                                                                                                                       aaaTATTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAAAGCAACGCTTCGGCACTGTTACCCCTGGTGCTCCAGCGCCTACTTATACAGCGACTTTTTCTTCACGCCGTGAAtt         >  2:49348/1‑251 (MQ=255)                                                                                                                                                                                                         atatTCCTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTc       <  1:320785/251‑1 (MQ=255)                                                                                                                                                                                                              ccgtttCAGAACGGTGTACTTCCTCTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGTCTAGTTAAGCTGTCCGTTGGTGAGCTGGAAACAGGAAAATTCCAGGGCACATCCATCATTCCCTGCATCGTGCGGTAAAAGAAGGCGTTGCGTTTTTATTAACGCAACGCTTCGGCACTGTTACCCCTGCGACTCTAGCGCGAACGTTTCCAGCGCATTTGTCTTGACGCCGTGAATTTCTGCgg  <  2:290776/248‑1 (MQ=255)                                                                                                                                                                                                               cTTTTCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCCTGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGACTTTCTGCgg  >  2:42800/1‑250 (MQ=255)                                                                                                                                                                                                                 tttCAGAACGGTATCCTTCCTGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCGGGCCAGTTAAGCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCgg  <  1:42800/248‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    GCCAGACGGTTTTACTCCAGTAGACTTTGATATCACTTATGACTGTGGTGATACTTCAAAGATTAAAAACTCGTTGCAAATGCGCATCGACGGTACAACTGGGGTAGTAGACCAGTACAACCTGGTCGCCAGGCGAAGAAGTTCAGACAATGTGCCCGATGTCGGTATTCGTATTGAAAATCTCGGCGGCGGAGTTGCAAATATTCCTTTTCAGAACGGTATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGG  >  NC_000913/3292084‑3292539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑