Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,259,283 G→A H123Y (CAT→TAT)  tdcG ← L‑serine deaminase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,259,2830GA97.9% 154.9 / ‑3.8 48H123Y (CAT→TAT) tdcGL‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (27/19);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

ATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCT  >  NC_000913/3259036‑3259523
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
aTTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCg                                                                                                                                                                                                                                               >  1:141431/1‑251 (MQ=255)
              gcgcATCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTc                                                                                                                                                                                                                                   <  1:203245/249‑1 (MQ=255)
                    cgcTAGCTCGTAGTGCATCATCAGACCAGATCTAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGCGTGGCAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATgg                                                                                                                                                                                                                            >  2:59405/1‑250 (MQ=255)
                              ttGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGTCAACATTTCGGGATGGAAGATAATGtt                                                                                                                                                                                                                 >  2:273968/1‑251 (MQ=255)
                                               aGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt                                                                                                                                                                                                >  1:264479/1‑251 (MQ=255)
                                               aGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt                                                                                                                                                                                                >  2:113833/1‑251 (MQ=255)
                                                        cAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGTAAAGCGTAAGGTACGGACGTTTCGCCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAatat                                                                                                                                                                                       <  2:141431/251‑1 (MQ=255)
                                                          ggCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATgc                                                                                                                                                                                     <  2:118205/251‑1 (MQ=255)
                                                                   aaTCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCTCTTCCAGGCAGTGATCCGCATTCCGTTCTCCTAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCAcc                                                                                                                                                                                >  1:287485/1‑247 (MQ=255)
                                                                      cacacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGcc                                                                                                                                                                         >  2:308168/1‑251 (MQ=255)
                                                                       acacaTTTTCAGCAGTTCAACTGCTGAGTGGCACTCGTAAGGCACGGACGATTCGACATCGCGCGACAGGCCGACGTGTTCTTCTTCGACAATACACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCCTTCCGTTCTCATAGCGAGGCACCATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGcc                                                                                                                                                                         >  2:59867/1‑250 (MQ=255)
                                                                       acacaGTTTCAGCAGTTCACCAGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGGTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATTAACAATATGCGCACCAGATGcc                                                                                                                                                                         >  2:279829/1‑250 (MQ=255)
                                                                         acaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACt                                                                                                                                                                      >  1:27915/1‑251 (MQ=255)
                                                                          caTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTg                                                                                                                                                                     >  1:237562/1‑251 (MQ=255)
                                                                           atttctagcagTTCACCTGCTGAGTGGAAAGCGTAAGGTACGGACGGTTCGACATCGTGCGACAGGTCGAAGTGTTCTTCTTCGACAATAAGCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGGGATCCGCATTCCGGTCTCATAGCGAGGCAACATGTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCGACTgg                                                                                                                                                                    <  2:395802/245‑1 (MQ=255)
                                                                                cagcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgcc                                                                                                                                                               >  1:83805/1‑251 (MQ=255)
                                                                                    agTTCCCCTGCTGAGGGGAAATCGTAAGGGACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGTTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGccgccc                                                                                                                                                           <  1:263517/251‑1 (MQ=255)
                                                                                      ttCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGccgcccgc                                                                                                                                                         >  2:125070/1‑251 (MQ=255)
                                                                                                   tGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTa                                                                                                                                             >  1:267630/1‑250 (MQ=255)
                                                                                                             aaGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAAt                                                                                                                                  >  2:364899/1‑251 (MQ=255)
                                                                                                                  aCGGCCGTTTCGACATGGTGAGACAGGCCGAAGTGTTCTTCGTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCGCTTACTGTCCCGTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAGCAGTTCGGGATGGAAGATAAGGTGCTTGGCGACAGGAAAAGCAACAACATGGGCACCATATGCGCCTGGCATCCTGCCGCGGCGCGTAACTAACGCGATAAATGCAg                                                                                                                              <  2:48180/250‑1 (MQ=255)
                                                                                                                  aCGGACGTTTCGACATCGTGCGACAGGCCAAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGAAGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAgg                                                                                                                             >  1:293781/1‑251 (MQ=255)
                                                                                                                                       gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCa                                                                                                                                                                                                                                          >  1:217066/1‑121 (MQ=255)
                                                                                                                                       gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCa                                                                                                                                                                                                                                          <  2:217066/121‑1 (MQ=255)
                                                                                                                                       gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCa                                                                                                                               <  1:59296/228‑1 (MQ=255)
                                                                                                                                       gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCa                                                                                                                               >  2:59296/1‑228 (MQ=255)
                                                                                                                                           ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAACATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGc                                                                                                    >  1:186319/1‑251 (MQ=255)
                                                                                                                                                 aGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTg                                                                                              >  1:192557/1‑251 (MQ=255)
                                                                                                                                                                  agaatcccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCGGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCGTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCTCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGTCTGTTTCCTGCCAGTCCCag                                                                             <  2:129799/246‑2 (MQ=255)
                                                                                                                                                                   tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCatg                                                                            >  1:317738/1‑251 (MQ=255)
                                                                                                                                                                   tAAACCCGCAGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGAATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCatt                                                                            >  1:177017/1‑250 (MQ=255)
                                                                                                                                                                      aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCatgatg                                                                         >  2:131471/1‑251 (MQ=255)
                                                                                                                                                                              ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGAc                                                                 <  1:273968/251‑1 (MQ=255)
                                                                                                                                                                               cgACAAAGTAATAGGGATTAATTAATAGCTATTCCTGGCCCTACCAGGCAGTGAGCCGAATTCCGATATCATAGCGAGGAAACATTTAGGGATGGAAGATAATGTGCTTTGCTACAGGAAAAGAAACAAAATGCGCACAAGATGACCCTGGCAGCCGCCAGCAGCGCGTTCCTAACTCTATAAATGCAGGGATCTCATCAATGAAAAAAACCTGAGAACTGTAGCATGCCAGTCCAATGATGACGGCgtca                                                                <  2:49443/251‑4 (MQ=255)
                                                                                                                                                                                 aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATc                                                              <  1:59405/251‑1 (MQ=255)
                                                                                                                                                                                      gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTgg                                                         <  1:222243/251‑1 (MQ=255)
                                                                                                                                                                                        aaTAGGTATTACGTAATAGCTCTACCGTTCCCATCCAGGCAGTGACCCGCATTCCGTTTTCATAGCGAGTCACCATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAAAGGCGCACCAGATGCCACTGGCAGCCGCCCACTGCGCGTTACAAACTCTATAAATGCGGGGCTCCTATCAATGTCAATATCCTGCGGACTGTTCCCTTCCAGTCACAGGATGATGGCGACATCCTTggta                                                       <  2:44280/251‑3 (MQ=255)
                                                                                                                                                                                            ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGAATTCCATTCACATAGCGAGGCAACATTACGGGATGGAAGATAATGTTCTTAGCTACAGGAAAATCATCAATATGCGCACCAGAAGCCACTGGATGCCGCCCGCTACACGCTACTAACTCTATAAATGCAGGGAACTCATCAATCACATCCTCCTCCGGACTGTTTCCGGGCTTTCGCATAATGATGGCGACATCCGTGGCATGGc                                                   >  1:388538/1‑251 (MQ=255)
                                                                                                                                                                                             gTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGcc                                                  >  1:178788/1‑251 (MQ=255)
                                                                                                                                                                                                     tAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGccc                                           <  1:7706/250‑1 (MQ=255)
                                                                                                                                                                                                      aaTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGt                                         >  2:113736/1‑251 (MQ=255)
                                                                                                                                                                                                              ttcctaaccaatCCAGGCAGCGATCCGCAATCCGATCTCATAACGCCGCAACATTTCCCGATGGAAGATACTGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTCACTCTATCAATGCAGGGATCTCATCAATGCCACCATCCTGCAGACTGTTTCCTGCCAGTCCCCTGATGATGGCGACATCCGTGGCATGGACTTTGCCCGTCAgcgcc                                  >  2:383534/12‑246 (MQ=255)
                                                                                                                                                                                                               tCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGAc                                  <  1:113833/249‑1 (MQ=255)
                                                                                                                                                                                                                              gcagtataccGCATTCCCTTCTCATAGCGAGGCAACATTTCGGAATGGAAGATAATTTTCTTTGCTACAGGAAAAACAACAATATGCGCCCCAAATGCCCCTGGCAGCCGCCCGCTGCGAGTTAATAACTCTATAAATGCAGGGAGCGCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATc                 <  2:139539/243‑1 (MQ=255)
                                                                                                                                                                                                                              gCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATc                 <  1:125070/251‑1 (MQ=255)
                                                                                                                                                                                                                               cagagagccGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCGCATCAATGCCAACATCCTGCGGTCTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCg                <  2:210424/244‑1 (MQ=255)
                                                                                                                                                                                                                                           ttCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCACCAATATGCCCACACGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATgg    >  1:267311/1‑251 (MQ=255)
                                                                                                                                                                                                                                             ccggactcATTGCGAGGCAACATTTCGGGATGGATGATAATGGTCTTTGCTACAGGAAAATCAACAATATTCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTCCTACCTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATTGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCt  <  2:184488/246‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
ATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGCT  >  NC_000913/3259036‑3259523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑