Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,423	C→T	G160G (GGC→GGT)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,423	0	C	T	100.0%	155.7 / NA	49	G160G (GGC→GGT)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

TATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGCTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGG  >  NC_000913/3240183‑3240663                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 tatCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCCGCTTTGCCTTCACTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGc                                                                                                                                                                                                                                        >  2:229269/1‑251 (MQ=255)                 ttCCTCTATCGACTGGGCACCTTCTCTGCTGCTCTGGCAGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGAAATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGGTTCTGGTGTGGGCGCTCGGCCTCGGTTTCTCACTGAGTCCCGGTAACGAGac                                                                                                                                                                                                                        >  2:16247/1‑251 (MQ=255)                  tCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTCACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTACCGAGacc                                                                                                                                                                                                                       >  2:130159/1‑251 (MQ=255)                                      ttCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACg                                                                                                                                                                                                    >  1:257558/1‑250 (MQ=255)                                          ctgctgCTCTGGCCGCAGGAGTCTTCAGCTTTGCCTTCCCGTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTTGTTAACGata                                                                                                                                                                                                 <  2:257558/249‑1 (MQ=255)                                                       cGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTACCGATATGTCGAATGCCGtt                                                                                                                                                                                   >  2:132025/1‑250 (MQ=255)                                                          agtagtCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCACAAACCTGGTTAACGATATGTCGAATGCCGTTACCt                                                                                                                                                                               >  1:65828/1‑251 (MQ=255)                                                               tCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATg                                                                                                                                                                          >  1:166461/1‑251 (MQ=255)                                                                       tttGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGtcatcttcttgaagaaac                                                                                                                                                                   >  1:376043/1‑233 (MQ=255)                                                                                ccttcTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCAttcg                                                                                                                                                         <  1:51410/251‑1 (MQ=255)                                                                                     tACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCAttcgcttcg                                                                                                                                                    >  1:148536/1‑251 (MQ=255)                                                                                      aCCCTGCATTTCTCAAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAAAGATATGTCGAATGCCGTTTCCTTTATGTTAAAACTGGTCAttcgcttcgc                                                                                                                                                   >  1:303959/1‑251 (MQ=255)                                                                                      aCCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCAttcgcttcgc                                                                                                                                                   >  1:69590/1‑251 (MQ=255)                                                                                          tGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTa                                                                                                                                                                                                       >  1:81671/1‑195 (MQ=255)                                                                                          tGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGCAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCg                                                                                                                                               >  1:180418/1‑251 (MQ=255)                                                                                          tGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCAGCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTa                                                                                                                                                                                                       <  2:81671/195‑1 (MQ=255)                                                                                           gCATTTATCTAGTAGCGCGGGTTATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGAGTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTTGCAcc                                                                                                                                                <  2:363296/249‑1 (MQ=255)                                                                                                     aGTAGAGCGGGGGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGCAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTAGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACATTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTAtttt                                                                                                                                    >  2:333924/1‑251 (MQ=255)                                                                                                        aGCGCGGGTGATATTGCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTTGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTTACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCTCCGATTGGTATTTTTg                                                                                                                                  <  2:138296/250‑1 (MQ=255)                                                                                                             gggTGATATTTCGCCGCCGTCAGGCATTGTCGCAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTAGAATGCCGTTACATTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGAATTTTTGGGCTgg                                                                                                                            >  1:297960/1‑251 (MQ=255)                                                                                                                 gATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTttc                                                                                                                        >  1:2948/1‑251 (MQ=255)                                                                                                                   tatTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTCTTTTTGGGATGGTttctt                                                                                                                      >  2:19564/1‑251 (MQ=255)                                                                                                                        cgcctcCGTCAGGCATTGTCGAAGTGATGCTCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGATAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTAc                                                                                                                  <  2:139267/250‑1 (MQ=255)                                                                                                                            gccgTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTgg                                                                                                             <  1:161339/251‑1 (MQ=255)                                                                                                                            gccgTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTCTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGTAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTgg                                                                                                             >  1:386206/1‑251 (MQ=255)                                                                                                                                  aGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCGGAAAGGTAACTACATCGGGATTCTGGTGTTGGCGATCGGCCGCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCTCCGATTGGTGTTTGTGGGCTGGTTTCTGCTACCCTTGCAacca                                                                                                       <  1:334713/251‑1 (MQ=255)                                                                                                                                       ttGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTg                                                                                                                                                                                                           <  1:340082/146‑1 (MQ=255)                                                                                                                                       ttGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACAACCAAAAACCTg                                                                                                                                                                                                           >  2:340082/1‑146 (MQ=255)                                                                                                                                                 gATGCGCGGGCTGGTAATGAGCATGGTTTCCACCCCCATCGACGCGCTGCTGAAAGTTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTCCCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCcaca                                                                                         <  1:173617/250‑1 (MQ=255)                                                                                                                                                 gATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCacac                                                                                        >  1:277340/1‑251 (MQ=255)                                                                                                                                                   ggcgcGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACtg                                                                                      <  1:130786/250‑1 (MQ=255)                                                                                                                                                                  tGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTAcc                                                                                                                                                                                >  1:139860/1‑146 (MQ=255)                                                                                                                                                                  tGAGCATGGTGTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTAcc                                                                                                                                                                                <  2:139860/146‑1 (MQ=255)                                                                                                                                                                            ttCCATCCCCATCGCCGCGCTTCTGAAAGGTAACTCCATCTGGTTTCTGTTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAATAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATTGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGTCAACCACCGTTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTc                                                              <  2:297960/250‑1 (MQ=255)                                                                                                                                                                              ccAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGt                                                            <  2:212141/250‑1 (MQ=255)                                                                                                                                                                                  ccccATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTgg                                                       >  1:301338/1‑251 (MQ=255)                                                                                                                                                                                        cGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCt                                                 >  1:192506/1‑251 (MQ=255)                                                                                                                                                                                          aCGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCGCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGt                                               <  1:229269/251‑1 (MQ=255)                                                                                                                                                                                            gctctgctgAAAGGAAACTACCGCGGGATTCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTTGTCAGTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTAt                                             <  2:148006/248‑1 (MQ=255)                                                                                                                                                                                               ctgctgAAAGGTAACTACATCGGGATTATGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGAACACCAAAAACCTGGTTAACGTTATATCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTATTCTACACTGACAACCACCGGTTTCTCAACACTGTGGGGCTACGCGCAACTGCCGGTCGTGCTGGTAGGCTGTATGtt                                          >  2:131372/1‑251 (MQ=255)                                                                                                                                                                                                     aaaGGTAACGACGTTGGGATTCTGTTGTGTGCGAACGTCCTCGGTTTCGCACTTCGTCACGGTAACGATACCTACAAAAACCTGGTTAAAGATATGTCGAATGCCGTTACCTTTAATGTGAAACTTGTCATTCTCTTCGCACCGATTGGTATTTTTGGGCTGTTTTCTTCAACTCTGGCGACCACCGGGATCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtggt                                    <  2:320294/251‑1 (MQ=255)                                                                                                                                                                                                         gTAACTACATCGGGATTCTTGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGgcg                                <  1:19564/251‑1 (MQ=255)                                                                                                                                                                                                         gTAACTACATCGGGAATCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACtggtgtcg                                >  2:263684/1‑248 (MQ=255)                                                                                                                                                                                                                        ttCTGGTGTGGGCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAAccc                  >  2:249429/1‑250 (MQ=255)                                                                                                                                                                                                                               gtgGGCGATCGGCCCCGGTTTCGCACTGCGTCACGGTAACGAGACCCCCAAAAACCTGGTTAACGATATGTCGAATGCCGTTCCCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCGTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGGCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCtgg          <  2:180418/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCtggtgtg      <  2:2948/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGATCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCtggtgtgg     <  2:192506/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTCGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCtggtgtggtg   >  1:224911/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGGCCTCGGTTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGtggtgg  <  2:237040/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 TATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCTCGGCTTCGCACTGCGTCACGGTAACGAGACCACCAAAAACCTGGTTAACGATATGTCGAATGCCGTTACCTTTATGGTGAAACTGGTCATTCGCTTCGCACCGATTGGTATTTTTGGGCTGGTTTCTTCTACCCTGGCAACCACCGGTTTCTCCACACTGTGGGGCTACGCGCAACTGCTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGG  >  NC_000913/3240183‑3240663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑