| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,224,895 | C→T | L755L (CTG→TTG) | ebgA → | evolved beta‑D‑galactosidase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,224,895 | 0 | C | T | 96.6% | 179.6 / ‑3.8 | 59 | L755L (CTG→TTG) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (28/29); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
AACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCG > NC_000913/3224652‑3225123 | aaCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcg < 1:202632/251‑1 (MQ=255) aTTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGa < 1:11280/251‑1 (MQ=255) aCGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGcc < 1:65452/251‑1 (MQ=255) gACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAg < 2:57845/250‑1 (MQ=255) cccAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGAt < 2:205735/250‑1 (MQ=255) aaGATTCCCGCACCCGCTCCAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAATACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGAGCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGTCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAAc < 2:265266/251‑1 (MQ=255) aGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGAGAATGGCGAATCGCTGTTGACTCGCGAGCCCAAGATCaaat > 2:188769/1‑249 (MQ=255) tCCCGCACCCGCAACAGCGAAGCCGGACACCCTATCGCTACTTAGCAGTTCACGCTGAAGGAAAACCCAGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCGGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGCCATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAActtct < 2:158513/250‑1 (MQ=255) cACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGACGGAAAACACCGCGCAGCCCGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTCCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCCAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGATCCCAAGATCAACTTCTTCAAGc > 2:179095/1‑251 (MQ=255) aCAGCGAAGCCCGACAACCTATTGCCACTTATCAGCTCTCGCTGAAGGAAAACACCGCGCAGCCAGTGGCTTTCGCACCAAATAATGCGCGTACCCAGACGCTGGAAGACGATCGCTTGCGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCCTCTAAAAAATGAGTGGCAGACCGACATCATGGCAGGTGAATGGCGAATCGCAGCTGACACGCGAGTCAAAGACAAAATTCTTCCAGACgatgaac > 2:351446/1‑249 (MQ=255) ccTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGAAAACCACACGCAgg > 1:236107/1‑250 (MQ=255) tATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAggg < 1:115994/249‑1 (MQ=255) tATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAAGGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAggg < 1:188769/249‑1 (MQ=255) cAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCtgt < 1:189430/250‑1 (MQ=255) cAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCtgt < 1:382136/250‑1 (MQ=255) ttCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAACTAATGCGCGTCCGCTGACGCTGGAAGACGCTCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAACGATCAACTTCTTCAAGCCGCTGATCGACACCCACAAGCAGGAGTA‑CGAAGGGCTGTGGcc > 2:332719/1‑250 (MQ=255) gAAGGAAAACCCCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGGCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTTTTGACTCGCGAGCCAAAGAGCAACTTCTTCAAGCCGATGATTGACAACCACAATCAGGAGTA‑CGAAGGGCTGTGGCAACCGAAt < 2:70152/250‑1 (MQ=255) gAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGGCCGCGGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTCCAACTTCGCGATCACCGTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCGTCAAGCCGATGATCTACAACCACAAGCAGGATTA‑CGAAGGGCTGTGGCAACCGAATc < 2:358395/251‑1 (MQ=255) aaaaCCCCGCGCCGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTg < 2:7569/251‑1 (MQ=255) acacCGCGCATCCAGTGCCTTGCGAACCAAATAATGCGCTTCCGCTGACGCTGTAAGACGATCGTTTGAGCTGCTCCGTTCGCGGCTACAACTTCGCAATCACCTTCTCAAAAATGAGTGGCAAACCGACAGCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTGCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAg < 2:290303/251‑1 (MQ=255) cgcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg > 1:152291/1‑251 (MQ=255) aGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTCCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGGGCCAACGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑GTAACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGc > 1:83145/1‑249 (MQ=255) cAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAAGGAGTGGCAAACCGACAGCCTTGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGaa < 1:198763/251‑1 (MQ=255) cAGTGCCTGTCTCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCTGCTACAACTTCGCGAGCACCTTCCCAAAAATGATTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAATGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGaa < 2:267320/251‑1 (MQ=255) aGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc > 1:15525/1‑251 (MQ=255) tttCTCACCAAATCATGCGCGTCCGCTGACGCTGGAAGACGTTCGTTTGAGCTGCAACGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACGCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACGGAATCATTTGCAGATCATGCAGGAACATCTgc > 2:136380/1‑251 (MQ=255) ttCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACTAGCGTTTGAGCTGCACCGTTCGCGGCTACAACGTCGCGATCACCTTCTCAAAAAGGGTTGGCAAACCGACATCCGGGCAGGGGAAGGGCGAATCGCTGTTGACTCGCGGGCTAAAGACCAGCTGCTTCAAGCCGATGGTCTTCATCCTTAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCAGTTGCAGATCAGGGAGGAACATCTg < 2:400009/249‑1 (MQ=255) ttCGCACCAAATAATGCGCGGCCGCTGACGCTGGAAGACGATCGTTTGAGCTGAACCGGTCGCGGCTACAACTTCGCGATCTCCTTCTCAAAAAGGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACCCGCGAGCCAAAGATCAACGTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTgc < 2:309802/250‑1 (MQ=255) aataatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGCGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGc > 2:343138/1‑251 (MQ=255) aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGAGACTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGCCATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGCCAACCCCAAGCAGGCGTA‑CGAAGGGCTGTGACAACCGAATCATTTGCAGAGCATGCAGGAACATCTGCGCGCCTTTGCCgc > 2:352355/1‑250 (MQ=255) cgcgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGa > 1:147481/1‑250 (MQ=255) ccGCTGTCGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCTCGAGCCAAAGATCAACTTCTTCAAGCCTATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTGGCCGTAGAACagag < 2:177640/251‑1 (MQ=255) ccGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACag > 1:205357/1‑249 (MQ=255) cGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGc > 2:322556/1‑251 (MQ=255) cGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt < 2:338143/251‑1 (MQ=255) gCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTg > 1:326660/1‑251 (MQ=255) aGACGATCGTTTGTGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc > 1:267906/1‑251 (MQ=255) aGACGATCGTTTGGGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTGCTCAAAAATGAGTGGCAAACCGGCATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGAGCAACGTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCGTGCAGGAACATCTGCTCGACTTTTCCGTAGAACAGAGCGATGGTGAAGTGc < 2:329544/251‑1 (MQ=255) aTCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGAGAATGGCGAATCGCTGTTGCCTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCGACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTCGACCAGAGCGACGGTGCAGTGCTGatc > 2:129290/1‑251 (MQ=255) cGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGtt > 2:49592/1‑250 (MQ=255) cgcgGCTACCACTTCGCGATCACCTGCTCAAACATGAGTGGCAAACCGACATCCTGGCAGGTGACTGGCGAATCGCTGTTGACTCGAGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAACACAGAGCGATGGTGAAGTGCTGAACATCATCCGcaacgttatagc > 2:277179/1‑241 (MQ=255) ggCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGcccc > 1:28178/1‑251 (MQ=255) acatcTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCccgcc < 1:8254/246‑1 (MQ=255) gATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCGGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACt < 1:398043/251‑1 (MQ=255) cctcctcAAAAATGTGGGGAAACCCCACATCCGGGAAGGTGAATGGCAAACCGCTGTTGCCTCGCGAGCCAAAGATCAACTCCTCCAAGCCGATGAACAACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGACCTGGCGGGAACACCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGTTATTGCCCCGCCGGTGTTTGACTTCggg < 2:199849/247‑1 (MQ=255) aaTGAGTGACAAAACGAAATCATGGCAGGGGAATGGCGAATCGCTGTTGACTCGCGAGCCACAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGCGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGc > 2:187712/1‑250 (MQ=255) aaaCGGACACCCGGGCGGGTGAAGGGCGAACCGTTGTTGACCCGCGAGCAAAAAATAAACTCCTGCAAGCCGATGAACGACAACCACAAGCAGGAGTC‑CGAAGGGCTGTGGCAACCGAATCATTGGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTg < 2:109335/251‑1 (MQ=255) aaCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGCCTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTGGAACAGCGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGc > 2:79336/1‑208 (MQ=255) aaCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGc < 1:79336/208‑1 (MQ=255) aaCCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTgg > 1:217927/1‑251 (MQ=255) gACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcgc > 1:264543/1‑250 (MQ=255) gACATACTGGCAGGTGAATGGCGACTCGCTGTTGACTCGCGCGCCAAAGATCCACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGACGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCAACAGCCGCACAGTTATTGCCCCGCCGGTGTTTGCCTTCGGGATGAGCTGCCCATACATCTGgcgcc > 2:372684/1‑250 (MQ=255) tCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGc > 1:360871/1‑251 (MQ=255) cgggCAGGTGATGGGCGAATCGCTGTTGACCCGCGACCAAAAGATCAACTTCTCCAAGCCGATGACCGACACCCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGTTGAAGTGCTGATCATCAGCCGCACATTTATGGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTg < 2:147481/249‑1 (MQ=255) cTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACagag < 1:157599/159‑1 (MQ=255) cTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTg > 1:136043/1‑251 (MQ=255) cTGGCAGGTGAATGACGACTCGCTGTTGACTCGCGAGCCAAAGATCAAATTCTTCAAGCCGATGATCGACAACCACAAGCCGGAGTA‑CGAAGGGCTGTGGCAACCGACTCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACagag > 2:157599/1‑159 (MQ=255) ggCAGGTGAATGGCGCATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGACGGGCTGTGGCAACCGAATCATTTGAAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGcc > 2:395900/1‑251 (MQ=255) cAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCg > 1:249631/1‑250 (MQ=255) | AACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTA‑CGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCG > NC_000913/3224652‑3225123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |