Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,617	G→A	intergenic (‑28/‑77)	folB ← / → plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,617	0	G	A	100.0%	188.9 / NA	54	intergenic (‑28/‑77)	folB/plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

CCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGC  >  NC_000913/3204373‑3204863                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ccaccaGCGCAAACCGCGCCCCCTCACCGTGTCTGACCACCGTTTCTGAAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCGGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATAcc                                                                                                                                                                                                                                                  <  2:150729/251‑1 (MQ=255)        cgcAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCg                                                                                                                                                                                                                                           <  1:192489/251‑1 (MQ=255)          cAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGc                                                                                                                                                                                                                                         >  1:398162/1‑251 (MQ=255)               cgcgCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCTCGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAAt                                                                                                                                                                                                                                    <  1:163557/251‑1 (MQ=255)                    ccccTCGACGTAGCTGACCACCGTTTATGCAATATCAGAGTATCTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCAATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGATGCTCTATAATTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAAtgag                                                                                                                                                                                                                                 >  2:246255/1‑247 (MQ=255)                      ccTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACCCTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGCAAGGTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGt                                                                                                                                                                                                                              >  2:277615/1‑250 (MQ=255)                       cTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGtat                                                                                                                                                                                                                            >  1:38939/1‑251 (MQ=255)                          gACGTGGCTGCCCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTACCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGtattat                                                                                                                                                                                                                         <  1:6435/251‑1 (MQ=255)                               ggCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTCTCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCcac                                                                                                                                                                                                                     >  1:183212/1‑250 (MQ=255)                                     cccccGTTTCTGCAATGTCAGCGTACATGAGGCAATCCGCCACATCATCACTTTTCGCCGCCTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCAGCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCa                                                                                                                                                                                                              <  2:92987/248‑1 (MQ=255)                                        ccGTTTCTGCATTGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCg                                                                                                                                                                                                           <  1:19630/251‑1 (MQ=255)                                            ttCTGCAATGTCAGCGTAACGGAGGCAATCCGCCACTTCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGTTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTTCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTg                                                                                                                                                                                                        <  1:235504/250‑1 (MQ=255)                                                gCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTATGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAacac                                                                                                                                                                                                   <  2:220083/251‑1 (MQ=255)                                                         gCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATCATCCACAGATTCATCGTTGAACACGAATTTTCa                                                                                                                                                                                          <  2:204013/251‑1 (MQ=255)                                                            tAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCaaa                                                                                                                                                                                        >  2:184454/1‑250 (MQ=255)                                                                      aaTCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCt                                                                                                                                                                             >  1:121026/1‑251 (MQ=255)                                                                         ccGCCACATCATCACTTTTCTCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTGCTGTTCGATGGTCTGTTCCCAGGCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATTCCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTa                                                                                                                                                                           <  1:397806/250‑1 (MQ=255)                                                                            ccACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAg                                                                                                                                                                       <  2:339316/251‑1 (MQ=255)                                                                            ccACATCATCAATTTTCGCCGCTTTACGGATCTCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCACTAGTTGTGTTTACCGACAGTTGCTCAATAAATAAAATATCCATCACGTCCTGCATGCTTTTTGGCTAACCAGATCCCACTTCCGGCGCAATGTGCGTATTATCCACAGATTCATCGTGGACCACGAATTTTCACAACGCCACAGCTTATg                                                                                                                                                                         >  2:285659/1‑249 (MQ=255)                                                                                atcatcACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGTCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGc                                                                                                                                                                    >  1:219362/1‑250 (MQ=255)                                                                                   atcaCTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGAGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGTTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGACTTTTCAAACCGGAACAGCTTATGCGTGCAATc                                                                                                                                                                >  2:113966/1‑251 (MQ=255)                                                                                       cTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCgcgc                                                                                                                                                            >  1:323875/1‑251 (MQ=255)                                                                                                     aCGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGTAATGATCCt                                                                                                                                               <  2:22716/250‑1 (MQ=255)                                                                                                         ttATCTCACACCATTTAGATATCGAACCCTCAATTATGTTAGATGGTCTGTTCCCAGTCGTAAAGGCCTCTCGAGGTGAATACCGCAAGTTGATCTCTAACTACAATATCCCTCACGTCCTGCCTACTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTCTGCGTGCAATCGCGCCTGGCATGATCCTCATcg                                                                                                                                          >  2:399358/1‑251 (MQ=255)                                                                                                                   ccATTTCGATATCTAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTAccgc                                                                                                                                  <  2:215218/249‑3 (MQ=255)                                                                                                                     atgtCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCTTCGCGTACCTCTGCg                                                                                                                              <  1:353346/248‑1 (MQ=255)                                                                                                                             atCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATTAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAt                                                                                                                       <  1:344068/250‑1 (MQ=255)                                                                                                                               cGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAATTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAttt                                                                                                                     <  1:397628/250‑1 (MQ=255)                                                                                                                               cGAACACTAAATTCTGTTCGATGGTATGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTCACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGCCTCCATTTc                                                                                                                    >  2:381904/1‑251 (MQ=255)                                                                                                                                gAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTcc                                                                                                                   <  2:84683/251‑1 (MQ=255)                                                                                                                                                 cGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGttt                                                                                                   <  1:124460/250‑1 (MQ=255)                                                                                                                                                  gATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGc                                                                                                 <  1:62775/251‑1 (MQ=255)                                                                                                                                                        cTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTtg                                                                                           >  1:183233/1‑251 (MQ=255)                                                                                                                                                              ccAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTg                                                                                                       >  1:269093/1‑233 (MQ=255)                                                                                                                                                              ccAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTggg                                                                                     >  1:211033/1‑251 (MQ=255)                                                                                                                                                              ccAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCAAGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTg                                                                                                       <  2:269093/233‑1 (MQ=255)                                                                                                                                                                        acacCAATATTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACTTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATc                                                                           <  2:398162/251‑1 (MQ=255)                                                                                                                                                                                               aaGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTgg                                                                                      >  1:272256/1‑217 (MQ=255)                                                                                                                                                                                               aaGTGGCTCTATAAATACAATAGCCATCACGTCCTGCCTTCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTGCCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTgg                                                                                      <  2:272256/217‑1 (MQ=255)                                                                                                                                                                                                        tataAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgcgc                                            >  1:112372/1‑250 (MQ=255)                                                                                                                                                                                                        tataAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgcgc                                            >  1:213220/1‑250 (MQ=255)                                                                                                                                                                                                          taAATACAATATCCATCACGTCCTGCCTGCTTATTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAAc                                         >  1:125296/1‑251 (MQ=255)                                                                                                                                                                                                                       cATCACGTCCTGCCTGATTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCAGAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCACTCCAGGCGCAACCAATGAGTTACGt                            >  2:80415/1‑251 (MQ=255)                                                                                                                                                                                                                        aTCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCAGCGCGTa                                                                                                                                      <  1:35620/144‑1 (MQ=255)                                                                                                                                                                                                                        aTCACGTCCTGACTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTa                                                                                                                                      >  2:35620/1‑144 (MQ=255)                                                                                                                                                                                                                              tCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAATTGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCGCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCATTGTGTTACGTATCGGTg                     >  2:309266/1‑251 (MQ=255)                                                                                                                                                                                                                               cctgcctgCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAATCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTg                     <  1:163120/250‑1 (MQ=255)                                                                                                                                                                                                                                     tgcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgc                                              <  1:195001/219‑1 (MQ=255)                                                                                                                                                                                                                                     tgcTTTTTGGCTAACCACATGCCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgc                                              >  2:195001/1‑219 (MQ=255)                                                                                                                                                                                                                                      gcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAgg               <  1:137980/249‑1 (MQ=255)                                                                                                                                                                                                                                        tttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCACAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGagc           >  2:380578/1‑251 (MQ=255)                                                                                                                                                                                                                                          tttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTATGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAgggcgc           >  2:133050/1‑246 (MQ=255)                                                                                                                                                                                                                                              gCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGt     <  1:184454/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaCCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGc  >  1:129655/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGC  >  NC_000913/3204373‑3204863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑