Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,193,351	G→A	G163E (GGG→GAG)	yqiK →	flotillin family inner membrane protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,351	0	G	A	97.8%	137.3 / ‑6.8	45	G163E (GGG→GAG)	yqiK	flotillin family inner membrane protein YqiK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/26);  minor base T (1/0);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

ATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAA  >  NC_000913/3193125‑3193588                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              aTTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCagaaga                                                                                                                                                                                                                       <  1:259441/251‑1 (MQ=255)                        cGATTTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGAAGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCATGAGGTTCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAAc                                                                                                                                                                                                <  2:257541/251‑1 (MQ=255)                              agtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGgag                                                                                                                                                                                           <  1:5011/250‑1 (MQ=255)                               gtagtCGCTTTCTTTATGCGGGTAACACCTTCAGTCGAAGGGATTGCCACCGCTGCCCAGCCGATGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTACGTGCAACAGCTGCGCAAATGACCATGCATGCTTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGACAGAAGACCTGCCGAAAAACGGTCTGGAACTgaag                                                                                                                                                                                           >  2:158965/1‑246 (MQ=255)                                 agtCGCTTTCTGTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACGGgagatc                                                                                                                                                                                        <  2:21773/250‑3 (MQ=255)                                        ttctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACATTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCa                                                                                                                                                                                  <  1:401326/249‑1 (MQ=255)                                                  gggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGGCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACttt                                                                                                                                                                      <  1:255639/251‑1 (MQ=255)                                                   ggTAAAACCTTCGGTAGAAGGGATTGCCACCGCTGCCCAGGCGCTGGGGCAACGCACCCTGTCTCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGGCCTGTGGAAGAACGGTCTAGAACTGGAGAGCGTTTCACGTACCAACTTTa                                                                                                                                                                     <  2:46837/251‑1 (MQ=255)                                                   ggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACttt                                                                                                                                                                      >  1:91272/1‑250 (MQ=255)                                                    gTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTaa                                                                                                                                                                    <  1:62322/251‑1 (MQ=255)                                                      taaacccccaataaaaGGGATTGCCAACGCTGCCCCGCCGCTGGGGCAACACAACATGTAGCCTGCAGACTCACGCATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGATGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGAGTCAGAAGACCTGTAGACAAACGGTCAGGAACTGGAGAGCGTTTCACTTACCCACTTTAAcc                                                                                                                                                                  >  2:62854/15‑251 (MQ=255)                                                       aaaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGCCGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAACTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAAcc                                                                                                                                                                  >  2:317280/1‑250 (MQ=255)                                                           cTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGAcc                                                                                                                                                             >  1:65730/1‑251 (MQ=255)                                                                    aaGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCGGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGa                                                                                                                                                     >  1:42074/1‑250 (MQ=255)                                                                            gCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCa                                                                                                                                             >  1:68393/1‑250 (MQ=255)                                                                              cACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACGTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATc                                                                                                                                          <  2:68393/251‑1 (MQ=255)                                                                                   cTGCCCCGACGCTGGGGCCACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGCGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAAACCTGACGCAAAACGGTCTGGAACTGGAGAGCGGTTCACGTACCAACTTTAACCAGAACTCGAAAGAACATTTCAATCCGAAc                                                                                                                                     >  2:324804/1‑251 (MQ=255)                                                                                                ggggCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACg                                                                                                                        <  1:375230/251‑1 (MQ=255)                                                                                                ggggCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACg                                                                                                                        <  1:162358/251‑1 (MQ=255)                                                                                                ggggCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAAAAATGCCTTTGACg                                                                                                                        >  1:236566/1‑251 (MQ=255)                                                                                                          aCCCTGTCGCCTGACGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGttt                                                                                                              >  1:68922/1‑251 (MQ=255)                                                                                                             cTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGTGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAAc                                                                                                           >  2:15599/1‑251 (MQ=255)                                                                                                                    cTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGATTAACCAAACTg                                                                                                    >  2:329758/1‑251 (MQ=255)                                                                                                                                  aTGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACaga                                                                                      <  1:81822/251‑1 (MQ=255)                                                                                                                                    gttggttgAAGATAAATGTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAgc                                                                                    <  1:158965/251‑1 (MQ=255)                                                                                                                                          tgAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGt                                                                               <  1:170215/250‑1 (MQ=255)                                                                                                                                                   aTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAAc                                                                     <  2:42074/251‑1 (MQ=255)                                                                                                                                                   aTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAAc                                                                     <  1:298046/251‑1 (MQ=255)                                                                                                                                                       gTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCACCTTTAACCAGACCTc                                                                                                                                                           >  1:75590/1‑161 (MQ=255)                                                                                                                                                       gTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTc                                                                                                                                                           <  2:75590/161‑1 (MQ=255)                                                                                                                                                             gcacTCCGTGCAACAGCTGCGCAAATGAACATGCATAAGTTACAGGATTCCCGCGAGAACTTTGTGCAGGAGGCGCAAAATACAGTGGCAGAAGACCTGGCGAAACACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTAAGGCAACAGAGAGCCGTAGCCGCGAACGTAACGAAGt                                                           >  2:279265/4‑251 (MQ=255)                                                                                                                                                                           aGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAg                                             <  1:48373/251‑1 (MQ=255)                                                                                                                                                                               gcgcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGCCCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAATTTGAACAGGATGTAGaa                                           <  2:272575/249‑1 (MQ=255)                                                                                                                                                                                  cAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGc                                      <  1:258387/251‑1 (MQ=255)                                                                                                                                                                                    aaTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCgg                                    <  1:386738/251‑1 (MQ=255)                                                                                                                                                                                          catgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTg                              >  1:379478/1‑251 (MQ=255)                                                                                                                                                                                             gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaa                            <  1:326306/250‑1 (MQ=255)                                                                                                                                                                                              catgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGGCCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaaaa                          <  1:370205/251‑1 (MQ=255)                                                                                                                                                                                               atgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGTAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaaaaa                         <  2:68922/251‑1 (MQ=255)                                                                                                                                                                                                      aCAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGCAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACCGGATGTAGAAGTTGCGGTGCGTGAAAAAAATcgcg                  >  2:287555/1‑251 (MQ=255)                                                                                                                                                                                                       cAGGATACCCGCGAGAACTTTGTGCAGAAGGTGCAAAATCCAGTGGCAGAAGACCTGTAGAAAAAAGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACCTTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATcgcg                  >  2:166045/1‑250 (MQ=255)                                                                                                                                                                                                                   gagaACTTTGTGCAGGAGGTGCGAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATcgcg     >  1:264588/1‑251 (MQ=255)                                                                                                                                                                                                                     gaACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGTAGAGCGTTTCACTTACCAACTTTAACCAGACCTCTAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACATGATTTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCa   <  1:65421/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCTTTGTGCAGTAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGACGCCGCGAACGTAACGAAGTTTAACAGGAGGTAGAAGTGGCGGTGCGTGAAAAAAATCGCGATGCGCGATCGCGCaa  <  2:56982/251‑1 (MQ=255)                                                                                                                                                                                                                       aCTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCa   >  2:110205/1‑249 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAA  >  NC_000913/3193125‑3193588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑