Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,121,033	T→C	I83V (ATT→GTT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,121,033	0	T	C	100.0%	94.7 / NA	29	I83V (ATT→GTT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/18);  total (11/18)

ATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCC  >  NC_000913/3120788‑3121269                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             aTTGGCAATCAGACACAGTCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAACGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGCACACCGACGCGACAATAACCCACg                                                                                                                                                                                                                                         >  2:169986/1‑251 (MQ=255)      cAATCAGACAAAGCCACGCCGCGAATCACGGTTTGAAGCCCAGGCCCACCAGCAGCGCCCCGATAATCGCCACCGGCGCACAAAAGCA‑AGCCGCGCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAACCGCTGATCGTCGGTGAAGGCGATAACCGAGCTACGGATCATATCGAAATGCCCGCTCGCGCCGGTGACTTTCTACAGGAACACCGCCGAGACACAAACCCACGCTAtt                                                                                                                                                                                                                                    >  2:166176/1‑251 (MQ=255)               ccacacccGCGGCGTAAACCGGTGTGAAGCCAAGGCCAACCAGCACCCCCCCGGTAATCGCCCCCGGCGCCCCAAAGCC‑AGCCGCTCCTCCCAGCAACGCCC‑CAAAGGAGAAA‑CCAAACAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATACCCGAGCTGCGGATAATACCGAACTGCCCGCTGGCAACGTTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAAt                                                                                                                                                                                                                           <  2:237855/246‑1 (MQ=255)                                      ttGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑AAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGAGCTTCGGTGAGGGAGATAACCGAGCTGCGGATAATATCGAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCg                                                                                                                                                                                                     <  1:247570/250‑1 (MQ=255)                                         aaGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCgcagc                                                                                                                                                                                                 >  1:106403/1‑251 (MQ=255)                                          aGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCgcagc                                                                                                                                                                                                 >  1:339548/1‑250 (MQ=255)                                              cAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCgcagcagca                                                                                                                                                                                             >  1:356309/1‑250 (MQ=255)                                                          cacccccccggtaaccacacccGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CAAATCAGTAACCCCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCGGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCaa                                                                                                                                                                                <  2:68450/231‑1 (MQ=255)                                                              gcACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCgg                                                                                                                                                                            <  1:332588/251‑1 (MQ=255)                                                              gcACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑AAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTAACCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCAGAAATAAAGCCATAGCCCGCAGCAGCAAAGGCCATATCAATCgg                                                                                                                                                                            <  1:116059/251‑1 (MQ=255)                                                              ccACCGGTAATCGCCACCGGCCCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATATCCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTAATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCgg                                                                                                                                                                            <  1:72326/250‑1 (MQ=255)                                                                   gtaaaccGCCACCGGCGCAC‑AAAGCCCAGCCGCTCCTACCA‑CAA‑ACACTCAAAAGAGAAAACCAACCAGTAACACCTGCAAACGCTTGTCGCCGGTGATGGAGATAACCGGTTTGCGGAAAAGATCGAACTGCCGGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCAATTGGCCATAATCCAGAAATAAAGCCAGAGCCCGCAGCAGCAAATGCCATATCAATCGTCAttt                                                                                                                                                                       <  2:237163/245‑1 (MQ=255)                                                                                     cccAAAGCC‑AGCCGCTCCTTCCAGCAACGCCC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCaa                                                                                                                                                     <  1:316716/250‑1 (MQ=255)                                                                                        aaaGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGAGCGTCGTTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGAAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCa                                                                                                                                                  <  1:193058/251‑1 (MQ=255)                                                                                            cc‑agccGCTCCTTCCAGCAACCCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGAGGGAGATAACCGAGCTGCGGATAATAGCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGgat                                                                                                                                              <  1:98287/251‑1 (MQ=255)                                                                                               agccGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACCCCTGAAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGgatag                                                                                                                                            <  2:106403/251‑1 (MQ=255)                                                                                                                         aaGGAGAAA‑CCAATCAGTAACACCTGCCAACGCTGATCGTCGGTGTAGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCTGCGACAATAACCCACGCTATTGGCCATGATCCATAAATAAAGCCATAGCCCGCAGCAGGAAATGCCATATCAATCGGCATTTTAAAGGCGAATCTTGCAATCAGGATAGCTAATATAAGGGTAATTGCTCCAGc                                                                                                                   >  1:246347/1‑251 (MQ=255)                                                                                                                          aGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATACTATAAGGGTTATTGCTCCAGCg                                                                                                                  >  2:45252/1‑251 (MQ=255)                                                                                                                           ggAGAAA‑CCAATCAGTAACACCTGCAAACGCGGATCGTCGGTGAGGGAGATACCCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGa                                                                                                                 <  1:352365/251‑1 (MQ=255)                                                                                                                              aaaa‑CCAACAAGTAACCCCTTCAAACGCTGATCGCCGGTGATGGGGATAACCGAGCTGCGGATAATATCGAACTGCCCTCTGGCAACGGTTAATTTATAAAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACAt                                                                                                              <  2:285063/250‑1 (MQ=255)                                                                                                                                                 cagcaacctcTGATGGTCGGTGATGGAAATAACAGAGCGGCGGAAAATAACTAACTGCCCGCTGGCAACGGTTAATTTAAACAGGAAAACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATGGCAATCAGGATAGATAATATAAGGGTTATTGCGCCAGCGACATGTCCTTTCAGACGTAATa                                                                                            <  2:61544/242‑1 (MQ=255)                                                                                                                                                      aagccTGAGCGTCGGTGAGGGAGATAACCGAGCTGCGAAGAATATCGAACTGCTCGCTGGCAACGGTAAATTTATAAAGGAACACCGCCGCGACAAAAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATAGCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGTGTATTGCTGCAGCGATATGTCCTTTCAGACGTAATACcgcg                                                                                       <  2:339501/247‑1 (MQ=255)                                                                                                                                                           tGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGc                                                                                  >  1:282429/1‑251 (MQ=255)                                                                                                                                                                   ggtgtgggAGATAACCGAGCGGCGGAGAATATCGACCTGCCCGCTGACAACGTTTAATTTATACAGAAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACgaaga                                                                          <  2:356309/245‑1 (MQ=255)                                                                                                                                                                                                                          attttaaacagtatcacCGCCGCGACAATAACCCACGCTATTGGCCATAAACCATAAAGAAAGCCATAGCCCGCAGCAGCAAAAGCCATATCAAGCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTAGTGCTCCAGCGACATGTCCTTGCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCTGATAGCCCCTGTCCTCCCAGCgg                   <  2:2088/237‑1 (MQ=255)                                                                                                                                                                                                                             ttctaCAGGAAAACCGCCGCAACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCCATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCATTTCAGACGTAATACAGCGAGTGCAACGCAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCat                >  2:236554/4‑251 (MQ=255)                                                                                                                                                                                                                             ttATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCat                >  1:40955/1‑251 (MQ=255)                                                                                                                                                                                                                                 acaagAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGGTCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATAc            <  2:110928/247‑1 (MQ=255)                                                                                                                                                                                                                                             ccgcGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCCGTCCTCCCATCGGCATATACATTTGGGTcc  >  2:184826/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             ATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCC‑AGCCGCTCCTTCCAGCAACGCAC‑CAAAGGAGAAA‑CCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCC  >  NC_000913/3120788‑3121269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑