Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,786	G→A	T165I (ACT→ATT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,786	0	G	A	97.0%	105.2 / NA	33	T165I (ACT→ATT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/22);  minor base T (0/1);  total (10/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATAC  >  NC_000913/3120550‑3121010                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  aGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCCCCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCGCCGGCGCAATATTGGCAATCAGa                                                                                                                                                                                                                     <  2:255574/251‑1 (MQ=255) aGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGa                                                                                                                                                                                                                     <  1:126375/251‑1 (MQ=255)           aaaGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACTTCGCTTTCACCCCTTTCCAGCCGTCCATAATTGCTACCAGCCAGAACGGCACAAGACCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCccg                                                                                                                                                                                                           <  2:295830/251‑1 (MQ=255)                        ccAGCCAAGCTTCCCCCAGAACCCACCGCCGCTGGCCACGTCTCTTTCCCCCCTTTCCAGCCGCCCACCATTGCTCCCAGCCAGAACGGCACAAGACCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACgg                                                                                                                                                                                              <  2:243037/250‑1 (MQ=255)                                         accAACCAGCCCCGCTGCCCACGCCTCGTTCCCCCCTTCCCAGCCGCCCATCTGTGCTACCAGCCAGACCGGCCCAAGAACCGACAGGAACGGAAACTGACGCCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTTGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCcca                                                                                                                                                                            <  2:259565/249‑1 (MQ=255)                                                    ccgcTGGCCCCGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGGATAACGGTTTGAAGCC‑CAGGCCCACCAGCAgcgc                                                                                                                                                                  >  2:235817/1‑250 (MQ=255)                                                     cgcTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGCCAGGAACGCTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCATCGCAc                                                                                                                                                                >  2:193058/1‑251 (MQ=255)                                                      gcTGCCCACGTCTCGTTCACCCCTTTCCAGCCGTCCACCATTGCTACCAGCCGGACCGGCACAAGAACCGACAGGAACGGTAACGGCCGCCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCAcc                                                                                                                                                               <  2:123705/251‑1 (MQ=255)                                                         ggccacgcttccttccccccTTTCAAGCCGCCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACTCACCAAACGCCACCGGCGCAATAGTGGCAACCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCg                                                                                                                                                              <  2:306115/233‑1 (MQ=255)                                                                     ttccccccTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAAGCGCCACCg                                                                                                                                                 <  1:150717/246‑1 (MQ=255)                                                                                 cAGCCGTCCATAATTGCAACCAGCCGGAACGGCACAGGACCCGACAGGACCGGTAACGGACGTCCCGCCAGTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                                                                                                    <  2:303281/251‑1 (MQ=255)                                                                                          atcatTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTcc                                                                                                                           >  2:116059/1‑251 (MQ=255)                                                                                            catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                                                                                                          <  1:231302/250‑1 (MQ=255)                                                                                            catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                                                                                                          <  1:143621/250‑1 (MQ=255)                                                                                                gctacaaaccagatcagCACCAGAACCAACAGGAACGGAACCTGCCGTCCCGACATTGCGCCAATGTGGCACGGATGGATTCCCGTTACATGACCGGAGACCAGAATCGGCACGCCCAACGCACCAAACCCCACCGGCGCCATATTGGCAATCAGACACAGACCCGCCGCGTATAACGGTTTTAAGCCACAG‑CCCACAAGCATCGCAACTGGAATCGCCACCGGCGCACCAAAGCCAGCCGATCCTTCCac                                                                                                                     >  2:25945/17‑250 (MQ=255)                                                                                                      agccggacgggcccacaacccGACAGAAACGGTAGCTGCCGTCCCGCCGGTGCGCCAATGTGGAACGGACCGATTCCCGTTCCCTGACCGGCGACCAGAACCGGCAGGCCCAACGCGCCAGACGCCGCCGGCACAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑GAGGCCCACCAGCAGCGCACCGGTAAGCGCCATCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGc                                                                                                               <  2:148110/232‑1 (MQ=255)                                                                                                           gAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCa                                                                                                           >  1:258642/1‑250 (MQ=255)                                                                                                                        cccGACATGAACTGAAACTGACTGCCCGCCATGGCGCCAAGGGGGACCGGAACGATTCCCGTTACCCGACCGGCGACCAGAATCGGCACGCCAAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAAt                                                                                             <  2:258642/250‑1 (MQ=255)                                                                                                                          cGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCa                                                                                           <  1:46140/251‑1 (MQ=255)                                                                                                                                  aCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGACCGGATAGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGAAACAcc                                                                                   >  2:53749/1‑251 (MQ=255)                                                                                                                                    ggTACCTGACGTCCCGCCATTCCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAACCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTg                                                                                 <  2:78274/251‑1 (MQ=255)                                                                                                                                     gTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAAAGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGc                                                                                >  1:110928/1‑251 (MQ=255)                                                                                                                                       aaCTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCa                                                                               <  1:301266/250‑1 (MQ=255)                                                                                                                                         cTGACGTCCCGCCATTTCGCCAATGTGGAACGGAGCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTGGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGgc                                                                                                                                               <  1:137461/184‑1 (MQ=255)                                                                                                                                         cTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGgc                                                                                                                                               >  2:137461/1‑184 (MQ=255)                                                                                                                                                      aTTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGAACAGAATCGGCACGCCCAACGCACCACACGCCACCGGCGCAATATTGGCAATCAGACAAAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCCGCCGCTCCTTCCAGCAACGCACCAAAGGAGAACCCAATCAGTAACACCTGCACACGCTGATCGTCGGt                                                               >  2:247570/1‑251 (MQ=255)                                                                                                                                                             cAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGgag                                                        <  1:235817/251‑1 (MQ=255)                                                                                                                                                                         gATCGATTCCCGTGACCTGACCGGCGACCAGAATCGGCACGCCCAACGCCCAAAACGCCACCGGCGCAATATTGGCAATAAGAAACAGCCCAGCCGCGTATAACGGTTTGAAGCC‑CAGGCACACCAGCAGCGCACCGGTAAGCGCAACCGGCGCACCAAAGCCAGCCGCTCCGTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTAGGTGATGTAGATAACCGAGCTg                                            <  2:176582/251‑1 (MQ=255)                                                                                                                                                                                        ccTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAAc                             <  1:277557/251‑1 (MQ=255)                                                                                                                                                                                         cTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACt                            >  1:285063/1‑251 (MQ=255)                                                                                                                                                                                                gccaCCAAAATCGGCACGCACAACCCACCAACCGCGACCGTGGCATAATTGGCAATCAGAACCAGCCCCGCCGCGTATAGCGGTTTGAAGCT‑AAGTCCCATCAGCAGCGCACCGGTAATCGCCGCCGGCGCACCAAAGCCAGCCGCTCCTTCGAGCATCGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAAGATCGAACTGCCCGCt                     <  2:360824/248‑1 (MQ=255)                                                                                                                                                                                                       gAATCGGCACTCCCAATGCCCCACACGCCGCCGGCGCAATATTGGCAACCAGACCCAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCTCCAGCAGCGCACCGGTAATCGCCACCGTCGCACCAAAGCCAGCCGCTCCTGCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGCTAATATCGTACTGCCCGCCGGCAACg              <  2:271426/251‑1 (MQ=255)                                                                                                                                                                                                                   ccAACGCACCAATCGCCACCGGCTCAATATGGGCAATCAGACACATCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGGCGGTGATGGAGATAACCGAGCTGCGGATAATTTCGTACTGCCCGCTGGCAACGGTTAAGTTATAc  <  2:383957/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  AGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCC‑CAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATAC  >  NC_000913/3120550‑3121010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑