Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,887	G→A	S152N (AGC→AAC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,887	0	G	A	97.6%	137.5 / ‑4.7	42	S152N (AGC→AAC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (18/23);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

TCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGG  >  NC_000913/3104642‑3105126                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                caaataacataCCCGCAGCCAGTGCAACTACGATGACGGTAAGATTACTATCGAAACCATCGACGGGAAAGAACGTGCCGCGCATTTGAGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATAGTACGATGGCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACTCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAaacaacg                                                                                                                                                                                                                                            <  2:247726/241‑2 (MQ=255)   aaTATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTc                                                                                                                                                                                                                                          >  1:85628/1‑251 (MQ=255)   aaTATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTc                                                                                                                                                                                                                                          <  2:294943/251‑1 (MQ=255)                 aGCCACCTCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACAGGGCAGCCGTTTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGAGTATctgct                                                                                                                                                                                                                            >  2:274581/1‑251 (MQ=255)                         cAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGCCGATCCGAGTTCGGTCGCTCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAAcc                                                                                                                                                                                                                    >  2:288401/1‑251 (MQ=255)                             ttCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAACACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTc                                                                                                                                                                                                                                          >  2:110793/1‑225 (MQ=255)                             ttCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACGCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTc                                                                                                                                                                                                                                          <  1:110793/225‑1 (MQ=255)                              ccGATGACGGTACGATTACTATCGAAACCATCGCCGGGACATAACCGGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTg                                                                                                                                                                                                               <  1:387500/250‑1 (MQ=255)                                      ggTACGATTGCTATCGAAAACATAACCGGGACAGAACATGACGCGCCTTAGCGCCGGGCAATTATCAACACGTTATTGATGGGTGACGATCCGAGTTAGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTAAGGTGGTGGCCAACACCGGGCATCCGATTCGCTGGGAAGGTCGCGCAGACAACTTAGCGGACTATATGCTGAAGAACCGTCGGAagctccg                                                                                                                                                                                                       >  2:202844/1‑246 (MQ=255)                                         aCGATTTCTATCGAAACCATCGCCGGGATCGAACCTGCCGCGCCTTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCgca                                                                                                                                                                                                     <  1:353314/250‑1 (MQ=255)                                             ttACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAAc                                                                                                                                                                                                >  1:251145/1‑251 (MQ=255)                                                          cATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatca                                                                                                                                                                                   <  2:352757/251‑1 (MQ=255)                                                           aTCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatcat                                                                                                                                                                                  <  1:50465/251‑1 (MQ=255)                                                                cGGGACAGAACCGGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACa                                                                                                                                                                             <  2:360905/251‑1 (MQ=255)                                                                 gggACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                              >  2:402055/1‑249 (MQ=255)                                                                 gggACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAg                                                                                                                                                                            <  1:166211/251‑1 (MQ=255)                                                                     cAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTc                                                                                                                                                                        >  2:128599/1‑251 (MQ=255)                                                                          ccTGCCTCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAATCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCAt                                                                                                                                                                   <  1:356485/251‑1 (MQ=255)                                                                                           ccGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAacc                                                                                                                                                  >  1:117473/1‑251 (MQ=255)                                                                                              gtcAATTATCAAAACGTTATTGAGGTGTGACGATCCGAGTTCGTGCGATCTCTTTTCCGACGTTTATGATATTACGATTTCGAAAGAACCTTTCCGTTACGGTCAGGTGGTGGCCAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACACCTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCTACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAaccacc                                                                                                                                               <  2:225246/249‑1 (MQ=255)                                                                                                               tATTGATGGGGGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACATTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAGCTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTACCATGGTGCCGAACCACCTTGATAACCGTgcgccc                                                                                                                              >  2:374724/1‑249 (MQ=255)                                                                                                                          gACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCgg                                                                                                                   >  1:277939/1‑251 (MQ=255)                                                                                                                             gATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCAt                                                                                                                <  1:92604/251‑1 (MQ=255)                                                                                                                              aTCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATg                                                                                                               >  1:208921/1‑251 (MQ=255)                                                                                                                                  gAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTcc                                                                                                           <  1:184386/251‑1 (MQ=255)                                                                                                                                                   aTTCCGACGTTGATGATATTACGAGTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGGCTGAAGAGCCGCAGCAACGGGCTGCGTATCTTCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGGCCGCCAGTCGTCACGGa                                                                                            <  1:257801/249‑1 (MQ=255)                                                                                                                                                      ccGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTGACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAatat                                                                                       <  1:288401/251‑1 (MQ=255)                                                                                                                                                       cGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATg                                                                                      >  1:98828/1‑251 (MQ=255)                                                                                                                                                          cGTTGATGATATTACGATTTCGAAAGAGCCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCg                                                                                   <  1:248838/251‑1 (MQ=255)                                                                                                                                                          cGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCg                                                                                   >  1:68693/1‑251 (MQ=255)                                                                                                                                                               atgatATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTtgat                                                                              <  1:81384/251‑1 (MQ=255)                                                                                                                                                                 gatATTAAGATTTCGAAAGAACATTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGGGCAGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGCACCCCCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGGCACGGAAATATGGCGTtgatg                                                                             >  2:263637/1‑250 (MQ=255)                                                                                                                                                                         gactgcGAAAGAACCTTTCCTATACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGGCGCGCAAACAGCTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGa                                                                    <  2:98828/246‑1 (MQ=255)                                                                                                                                                                                aaaGAACCTTTCCTTTAAGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGCGCCGCAGCAACGGGCTGCGAATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGc                                                             >  2:384343/1‑251 (MQ=255)                                                                                                                                                                                 aaGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGc                                                             >  2:109621/1‑250 (MQ=255)                                                                                                                                                                                        tttcatttACAGTCAGACGGTGGACCTCACCGGGCAGCCGATTCGACGGGCAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGCAAAACCGTCTGAGGAGCCGCCGCGGCGGGCTGCGTATCATCTACCGCGTCACCATTCACATGGTGCCGAACCACCTTGATAACAGTGCGCACAACTATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGCTGAGTCGCTGATTCTGGCAATTATGc                                                     >  2:187122/1‑251 (MQ=255)                                                                                                                                                                                                 cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATa                                                                                                                                         >  2:276006/1‑158 (MQ=255)                                                                                                                                                                                                 cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATa                                                                                                                                         <  1:276006/158‑1 (MQ=255)                                                                                                                                                                                                 cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAt                                            <  1:298930/251‑1 (MQ=255)                                                                                                                                                                                                              gACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAAccc                               <  1:260809/251‑1 (MQ=255)                                                                                                                                                                                                                        ggCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTc                     <  1:195450/251‑1 (MQ=255)                                                                                                                                                                                                                        ggCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCGTCCTTTAACCCGTATGCGGTc                     <  2:70225/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGGTCGCGCAAACAACTTCGCGTATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCAGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTgg  <  2:13153/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGG  >  NC_000913/3104642‑3105126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑