Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,090,968 G→A E208K (GAG→AAG)  endA → DNA‑specific endonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,090,9680GA97.1% 225.4 / NA 68E208K (GAG→AAG) endADNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/39);  minor base C (2/0);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAA  >  NC_000913/3090721‑3091210
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
aGTCGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGt                                                                                                                                                                                                                                                  <  1:195522/251‑1 (MQ=255)
   cGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGc                                                                                                                                                                                                                                                <  1:281681/250‑1 (MQ=255)
   cGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGGCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTAAATGCGCGACCAATACAACCCGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCg                                                                                                                                                                                                                                               >  1:91357/1‑251 (MQ=255)
    ggtgaggtgaATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGGACGGTCAATGCGCCATGAAGGGCGATTTCCAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCCCCTACTTCTATATGCGCGGCCAATACAACCTGACACTCTCTCGCCAACCAACGCCGCTGTTCAACGCCTGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCgg                                                                                                                                                                                                                                              >  2:233028/1‑250 (MQ=255)
     gtgaggtgaATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑Ag                                                                                                                                                                                                                                            <  2:128692/251‑1 (MQ=255)
        aggtgaATGGCGATCGCGGCAACTTTATGGACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCGCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑Agcgc                                                                                                                                                                                                                                         <  2:232562/251‑1 (MQ=255)
                 gCGATCGCGGCAACTTTATTTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGGAAACGCAGCTGTTCAACGGATGGAACTAGATGTATCCGTTTACCGACTGGAAGTGCG‑AGCGCGATGAACGc                                                                                                                                                                                                                                <  1:348303/251‑1 (MQ=255)
                    aTCGCGGCAACTTTATGTACAGCCAGTGGAATGGCTGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATc                                                                                                                                                                                                                             <  1:154671/251‑1 (MQ=255)
                    aTCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGGCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTGCAACGCATGGAACAAGATGTATCCGGTTACCGACGGGAAGTGCG‑AGCGCGGGGAACGCATc                                                                                                                                                                                                                             <  2:185745/251‑1 (MQ=255)
                      cgcgGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGGCTGGAAGGGCG‑AGCGCGATGAACGCATcgc                                                                                                                                                                                                                           >  1:210103/1‑251 (MQ=255)
                            aaCTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGt                                                                                                                                                                                                                     <  1:4036/251‑1 (MQ=255)
                            aaCTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGt                                                                                                                                                                                                                     <  2:360950/251‑1 (MQ=255)
                             aCTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCGTTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGGTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTg                                                                                                                                                                                                                    <  2:205775/251‑1 (MQ=255)
                                        aGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGACGGTCGATTTCAAAGAAAAAGCTGCCGAACCCCCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCCGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATc                                                                                                                                                                                                          >  1:186929/1‑250 (MQ=255)
                                        aGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGGCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCCATACAACCTGAAACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCCTCGCGAAGGAGCAgggacatc                                                                                                                                                                                                          >  1:61343/1‑245 (MQ=255)
                                             gTGGAATGGCGGTGAAGGACAGTAAGGGCAATGCGCCATGAAGGTCGGTTTTAAAGAAAAAGCGGCCGAACCACCAGCGCGGGCACGCGGTGCCCTTGCGCGCACCTACTTCTATATGCGCGACCAATACAAACTGACACTCTCTCGCCCGCAAACGCAGCCGTTCCAAGCATGGAACAAGATGTAACCGGATACCGACTGGAAGTGCGGAG‑GCGATGGACGCATCGCGCAGGGGCAGGGCAAGAATAAcc                                                                                                                                                                                                    >  2:107023/1‑251 (MQ=255)
                                              tGGAATGGCGGAGAAGGCCAGTACGGTCAATGCGCCATGAAGGGCGATTTTAAAGAAAAAACTGCCGAACCACCAGCGCGCGCACGCGGTGCCATTGCGCGCACCTACTTATATATGCGCGACCAATACAAACTGACACTCTCTCGCCAGCAAACGCAGCTGGTCAACGCATGGAAAAAGCTGTATCCGGTTACCGACTGGCAGGGCG‑GGCGCGATGAACGCATCGCGAAGGTGCCGGGCCAACATAAccc                                                                                                                                                                                                   >  2:50409/1‑251 (MQ=255)
                                                                    aCGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGACATCATAACCCGTATGTGCAACGCGCTTgac                                                                                                                                                                               >  2:278136/1‑247 (MQ=255)
                                                                     cGGTCAATGCGCTATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCTCCTACGTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGGTCAACGCATGGAACAAGATGTATCCGGTTACCGACGGGAAGTGCG‑AGCGCGATGAACTCATCGCGAATGTGCAGGTCAATCATAACCCGGAGGTGCagggcgtgtgccagg                                                                                                                                                                            <  2:231858/251‑15 (MQ=255)
                                                                       gTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgcg                                                                                                                                                                          <  1:284790/251‑1 (MQ=255)
                                                                       gTCAATGCGCCAGGAAGGGCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTGTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgcg                                                                                                                                                                          <  2:229279/251‑1 (MQ=255)
                                                                         cAATGCGCCATGAAGGTCGCTTTCAACGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGACGGTGCCGGGCACTCATAACCCGTATGTGCAACGCGCTTGCCAGGcgcg                                                                                                                                                                        >  2:352831/1‑251 (MQ=255)
                                                                                 cATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCt                                                                                                                                                                <  2:210103/251‑1 (MQ=255)
                                                                                      aGGTCGCTTTGAACGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTCCTTCTATATGCGAGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTAACGACTGGAAGTGCG‑AGCGCGATGACCGCCTCGCGAAGGTGCAGGGCACTCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAAcc                                                                                                                                                            >  2:64537/1‑250 (MQ=255)
                                                                                        gTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTac                                                                                                                                                         <  1:249127/251‑1 (MQ=255)
                                                                                            aTTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTTCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCGGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCTCGTAGGTGCAGGGCAATCATAGCCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTa                                                                                                                                                     <  1:28689/251‑1 (MQ=255)
                                                                                              ttCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACTTACTTCTATATGCGGGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATTTAGCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGTTGCAGGGCAATCATAACCCGTATGGGCTACGCGCTTGCCGGGCGCGAAAGAGCTTACCGACACTAg                                                                                                                                                    <  2:167590/250‑1 (MQ=255)
                                                                                               tCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCACCGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCg                                                                                                                                                  >  2:107086/1‑251 (MQ=255)
                                                                                                   aGAAAAAGCTTCCGAACCACCAGCGCGTTCACCCCGGGCCATTGCTCGCACCTACTTCTATATGCGCGACAAATACAACCGGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCAGGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCGACACTAGCTGGAt                                                                                                                                              <  2:268984/251‑1 (MQ=255)
                                                                                                      aaaaGCTGCCGAACCCCCAGCGCGTTCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGTCCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCTAAAGAGCTAACCTACACTAGCGGGATTCt                                                                                                                                           <  1:218187/251‑1 (MQ=255)
                                                                                                          gCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCttttt                                                                                                                                       <  1:254135/251‑1 (MQ=255)
                                                                                                            tGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATCTGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGt                                                                                                                                     >  1:349358/1‑251 (MQ=255)
                                                                                                                 aaCCACCAGCGCGTGCACGCGGTACCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAAcc                                                                                                                                >  2:157806/1‑251 (MQ=255)
                                                                                                                          cgcgTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTAcccc                                                                                                                        >  1:9220/1‑250 (MQ=255)
                                                                                                                           gcgTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCAc                                                                                                                      <  1:231915/251‑1 (MQ=255)
                                                                                                                              tGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCAcgcg                                                                                                                   >  1:232227/1‑251 (MQ=255)
                                                                                                                               gCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGt                                                                                                                  >  1:281265/1‑251 (MQ=255)
                                                                                                                                 ccgcgGTGCCATTGCGCGCCCCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTAc                                                                                                                <  2:222206/250‑1 (MQ=255)
                                                                                                                                  cgcgGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACa                                                                                                               <  1:97413/251‑1 (MQ=255)
                                                                                                                                     ggTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAAcc                                                                                                            >  2:33777/1‑251 (MQ=255)
                                                                                                                                          cATTGCGCGCCCCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACACCCGCGTg                                                                                                       <  2:106670/251‑1 (MQ=255)
                                                                                                                                           attgtacgcAACTACTTCTATATGCGCGACCAATACAACCTGACACTCTATCGCCAGCAAACGCAGCTGTACAACGCATGGAACACGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGCAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTgg                                                                                                      >  2:268100/7‑251 (MQ=255)
                                                                                                                                              gcgcgcACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTgggg                                                                                                    <  1:107023/250‑1 (MQ=255)
                                                                                                                                               cgcgcACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGgag                                                                                                  <  2:281265/251‑1 (MQ=255)
                                                                                                                                                     ccTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGacgacg                                                                                            <  1:134620/251‑1 (MQ=255)
                                                                                                                                                            cTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGCCGACGCGGAttt                                                                                     >  2:374736/1‑251 (MQ=255)
                                                                                                                                                                  gcgcGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACt                                                                               >  1:119180/1‑251 (MQ=255)
                                                                                                                                                                  gcgcGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTACACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGAGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGCCGACGCGGATTTTTAACt                                                                               >  1:182420/1‑251 (MQ=255)
                                                                                                                                                                       aCCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCCACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCg                                                                          >  1:234135/1‑251 (MQ=255)
                                                                                                                                                                          aaTACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTGTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTAttcttat                                                                       <  2:91343/251‑7 (MQ=255)
                                                                                                                                                                                aCCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCg                                                                 <  2:119180/251‑1 (MQ=255)
                                                                                                                                                                                aCCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGAATCCGGTTACCGAATGGCAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTGACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCg                                                                 >  1:237558/1‑251 (MQ=255)
                                                                                                                                                                                    gACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGCCGACGCGGATTTTTAACTATGCGTATCCCCCGcctt                                                             >  2:219740/1‑248 (MQ=255)
                                                                                                                                                                                    gACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCAtt                                                             >  1:128908/1‑251 (MQ=255)
                                                                                                                                                                                      aacTCTCTCGCCAGCAAACGCAGAGGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCTAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTa                                                           <  2:297489/250‑1 (MQ=255)
                                                                                                                                                                                       acgctctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTat                                                          <  1:352831/248‑1 (MQ=255)
                                                                                                                                                                                           tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTatcatc                                                      >  1:217943/1‑251 (MQ=255)
                                                                                                                                                                                               gCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTg                                                   >  1:156073/1‑250 (MQ=255)
                                                                                                                                                                                                                          ggAACAAGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATcgc                       >  1:197264/1‑251 (MQ=255)
                                                                                                                                                                                                                               aaGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGGGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACGAGCGGGATTCTGTTTGTTAACCCCTACCCCACGCTTACAACCGCGTggg                                                                                                     <  2:373630/168‑1 (MQ=255)
                                                                                                                                                                                                                               aaGATGTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACACGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTggg                                                                                                     >  1:373630/1‑168 (MQ=255)
                                                                                                                                                                                                                                aGATGTAGCCGGTTACCGACGGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGGCGCGGATTTTTAACTATGCGTATCCCCCGCGTTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTg                 <  1:377143/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gTATCCGGTTACCGACTGGAAGTGCG‑AGCGCGAGGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGaa             <  2:217943/251‑1 (MQ=255)
                                                                                                                                                                                                                                        ccGGTTACCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATg         <  1:157806/251‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATgccgcc    <  1:278136/250‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCa   <  1:233028/251‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCa   <  1:205686/251‑1 (MQ=255)
                                                                                                                                                                                                                                               ccGACTGGAAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCaa  <  1:153027/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
AGTCGGTGAGGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGGAATGGCGGTGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCG‑AGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAA  >  NC_000913/3090721‑3091210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑