Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,065,861 G→A V354I (GTC→ATC)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,065,8610GA98.0% 163.4 / NA 49V354I (GTC→ATC) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/24);  minor base C (1/0);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGG  >  NC_000913/3065628‑3066105
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
cGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGat                                                                                                                                                                                                                                     >  1:120452/1‑251 (MQ=255)
 ggTAAGTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGata                                                                                                                                                                                                                                    <  2:274362/251‑1 (MQ=255)
    aaTTCCTCCGTTTATGATGTATTCTGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATAtt                                                                                                                                                                                                                                  <  2:15192/250‑1 (MQ=255)
       tcctccGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTAc                                                                                                                                                                                                                               <  1:298292/250‑1 (MQ=255)
             gTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTATGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATg                                                                                                                                                                                                                        >  1:340662/1‑251 (MQ=255)
               ttATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTTCGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGcc                                                                                                                                                                                                                      >  1:19662/1‑251 (MQ=255)
                 atgatgTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCa                                                                                                                                                                                                                     <  2:4389/250‑1 (MQ=255)
                          tCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAacac                                                                                                                                                                                                            >  1:360886/1‑250 (MQ=255)
                            ggAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACg                                                                                                                                                                                                         <  1:19864/251‑1 (MQ=255)
                                gTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGc                                                                                                                                                                                                     <  1:229286/251‑1 (MQ=255)
                                   ctaaaGGAATCGGAGCTGCATTTACCGGAAACCGGCCAACTCAGCGGGGCCAGCGCCTCCAGCCTGACACTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCACATAACCCGGAAATCATCCGTCGACTGGGCGTCATCGCACTGAAACTCGGCATGGAGTTTGATATTTACGTGCATGCCAACTCTACCCACGTAGCCgg                                                                                                                                                                                                  >  2:229591/5‑251 (MQ=255)
                                   cTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATCTTTACGGGCATGCCAACTCAACACACGTAGCCgg                                                                                                                                                                                                  >  2:175102/1‑251 (MQ=255)
                                               gtggtgCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCtgat                                                                                                                                                                                      >  2:158697/1‑251 (MQ=255)
                                                    gCATTTACTGCAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGCGTTTGCTATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACg                                                                                                                                                                                 >  1:279297/1‑251 (MQ=255)
                                                     cATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTGGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGGCATCGCGCTGAACAGCGGCCTGGAGTTTGATAGTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACgg                                                                                                                                                                                <  1:237942/251‑1 (MQ=255)
                                                      aTTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGt                                                                                                                                                                                               >  1:339683/1‑235 (MQ=255)
                                                      aTTTACTGGAAACCGGCAAAAGCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGt                                                                                                                                                                                               <  2:339683/235‑1 (MQ=255)
                                                           cTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATcgg                                                                                                                                                                          >  2:139863/1‑251 (MQ=255)
                                                             ggAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATcggcg                                                                                                                                                                        <  1:227982/251‑1 (MQ=255)
                                                                        aaaTCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGAt                                                                                                                                                             >  1:42523/1‑251 (MQ=255)
                                                                            cAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGTCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTg                                                                                                                                                         <  1:392980/251‑1 (MQ=255)
                                                                                    ccAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGaacgcaac                                                                                                                                                 <  1:175102/251‑1 (MQ=255)
                                                                                                       aaTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTa                                                                                                                              <  1:42950/251‑1 (MQ=255)
                                                                                                       aaTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATCACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCttt                                                                                                                               >  2:333946/1‑250 (MQ=255)
                                                                                                                  aTTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCg                                                                                                                   <  1:282773/251‑1 (MQ=255)
                                                                                                                  aTTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCg                                                                                                                   >  1:342696/1‑251 (MQ=255)
                                                                                                                    ccccTGCGCAAGAGTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCTTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGAt                                                                                                                 <  1:159963/250‑1 (MQ=255)
                                                                                                                     cccTGCGCAATATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGAt                                                                                                                 <  1:59546/250‑1 (MQ=255)
                                                                                                                         gcgcAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCt                                                                                                             <  1:158697/250‑1 (MQ=255)
                                                                                                                                  ttACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGCTCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGGTCTTTATGGGCCCGTCGATTGCTAAAGAAGGc                                                                                                    >  1:47920/1‑250 (MQ=255)
                                                                                                                                             tGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAg                                                                                                                                                                                                      >  2:98871/1‑141 (MQ=255)
                                                                                                                                             tGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAg                                                                                                                                                                                                      <  1:98871/141‑1 (MQ=255)
                                                                                                                                              ggATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAAcc                                                                                        <  1:274916/250‑1 (MQ=255)
                                                                                                                                              ggATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACcg                                                                                       >  1:268529/1‑251 (MQ=255)
                                                                                                                                                 ttACTTTGCCAGCCGCATTGTGATGCGTCCGCAGAAGATTTCAAATAACCCGGAAATCATCAGTCGTCTGGGCGTCATCGCTCTGAAAATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGAGGTGATTTTGAACGCAACGCGTATATGTCGATCTTTATGGCACCGTCGATTGCTAAAGACGGCAAGCTCTCAACcgtcg                                                                                    >  2:52607/1‑251 (MQ=255)
                                                                                                                                                   acgttGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGTCAGCGGTGATTTGGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACcgtcgt                                                                                   <  1:229591/247‑1 (MQ=255)
                                                                                                                                                       tGCCAGCCGCATTGTGTTGCGTCCGCAGTAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCCACACACGTAGCCgggg                                                                                                                                                                                                >  2:143706/1‑137 (MQ=255)
                                                                                                                                                       tGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCgggg                                                                                                                                                                                                <  1:143706/137‑1 (MQ=255)
                                                                                                                                                            gCCGCATTGTGGTGCGTCCGCAGGAGATTGCCAATAACCCGGAAATCAGCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGc                                                                         <  2:335338/251‑1 (MQ=255)
                                                                                                                                                             ccGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGc                                                                         >  1:266938/1‑250 (MQ=255)
                                                                                                                                                              cGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGGCGTGCCAATGTGc                                                                         >  2:298963/1‑249 (MQ=255)
                                                                                                                                                                            tccgccagagaTTTCCAATAACCCGGAAATCAACCGTCGTCTGGGCGTCATCACTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCAGGGGTCGATCTGCTGAACGGAATCGGCGGCAGCGGCGATTTTGAACGCAACGAGTATCTGTCGATCTTTATGGCCCCGTCGATTTCTAAAGAAGGCAAAATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATcac                                                          >  2:337605/8‑250 (MQ=255)
                                                                                                                                                                               gCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCg                                                      >  1:167238/1‑251 (MQ=255)
                                                                                                                                                                                                      aaaTCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATTCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGatcatc                               <  1:157016/251‑1 (MQ=255)
                                                                                                                                                                                                                       ggCGTCATCGCTCGGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGAGCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGc              <  2:172120/251‑1 (MQ=255)
                                                                                                                                                                                                                            cATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCCACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCCATGTGCAGCCATGTTGATCACAGCGAAAACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGaac         >  1:348370/1‑249 (MQ=255)
                                                                                                                                                                                                                                  tctGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGatc                                  >  1:10536/1‑220 (MQ=255)
                                                                                                                                                                                                                                  tctGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGatc                                  <  2:10536/220‑1 (MQ=255)
                                                                                                                                                                                                                                   ctGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGCTTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATATTGATCACAGCGAAAACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGGTCTGCTCgg  >  2:301127/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
CGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGG  >  NC_000913/3065628‑3066105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑