Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,449	G→A	L216L (TTG→TTA)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,449	0	G	A	95.0%	125.5 / NA	43	L216L (TTG→TTA)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (18/20);  minor base C (1/1);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAC  >  NC_000913/3065210‑3065694                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCg                                                                                                                                                                                                                                             <  2:211002/250‑1 (MQ=255) cGAGTCTGGTTAACCAGCGGGAGCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATATTCCATGCAATGGATCGAGTAGGTACACGCTATGTGCAAATCGATCCGAAAAAGATTGGTGCCGTCGAGGAAAACAACTTACCCCACGCCgg                                                                                                                                                                                                                                            >  1:220608/1‑251 (MQ=255)   aGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGAACCGAAAAAGATTGGCGCCGTCGTGGAAACCAACTTACCCGACGCCGGt                                                                                                                                                                                                                                           >  1:116765/1‑250 (MQ=255)    gTCTGGTTAACCAGCGGGATCGGTAATGCGCCGCCCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGt                                                                                                                                                                                                                                           >  1:108317/1‑249 (MQ=255)    gTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCTCGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTTTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTaa                                                                                                                                                                                                                                         <  2:323725/251‑1 (MQ=255)    gTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGt                                                                                                                                                                                                                                           <  2:108317/249‑1 (MQ=255)       tGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAatat                                                                                                                                                                                                                                      >  2:2712/1‑251 (MQ=255)         gTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGc                                                                                                                                                                                                                                    <  2:70210/251‑1 (MQ=255)           tAACCAGCGGGATCGGTAAAGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAACCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAATCCAACTTACCCGTCGCCGGTAATATGCt                                                                                                                                                                                                                                   <  2:230791/250‑1 (MQ=255)              ccAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGAt                                                                                                                                                                                                                               <  1:381700/251‑1 (MQ=255)                                   cGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGAAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGGGTCGATCTTCCATGCAATGGATCGCGTCGGGACCCGCTATGTGCAAATCGATCCGAAAAAGGTTGTCGCAGTCGTGGAAACCAACTTACCCGAAGACGGTAATATGCTGGATAAGCAAAATCCCAtgtgacac                                                                                                                                                                                                          >  1:6679/1‑247 (MQ=255)                                      ccTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagcag                                                                                                                                                                                                       <  2:301489/251‑1 (MQ=255)                                         ggcggcttcggggttaggacgTGATCATTTTACTCAAACGCGATCACGATCCGCGCATTGCAGAACGGTCGGATATTGTG‑ATTTCTGGCTCGCCTCCGCGGAGCAATATCTTGTCGTTCTTCCATTCAATGGATCGCGTAGGTACACGCTATGTGCAAATCGATCCGAAAAAGAGTGTCGCCGTCGTGGAAACCAACTTCGCCGACGCCGGTAATATGCTGGATAAGCAAAAACTCATTTGCCAGCAGAtg                                                                                                                                                                                                    <  2:273066/231‑2 (MQ=255)                                         ggCTTCTGCTGGCTAATAAAGTGATCATTTAACTCAAGCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATGGTG‑ATTCCTGGCGCGCCACCGCGTCGCAATAGTGTTTCTATATTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAATATTGTCGCTGTCGTGGAAACCAACTTACCCGAGGCCGGTTATATGCGGTATAAGCAAAAGCCCATGTGCcaggatata                                                                                                                                                                                                    <  2:357124/251‑7 (MQ=255)                                                     cGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTg                                                                                                                                                                                        <  1:243822/251‑1 (MQ=255)                                                         gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCa                                                                                                                                                                                    <  1:304046/251‑1 (MQ=255)                                                          aaaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCAc                                                                                                                                                                                   <  1:337777/251‑1 (MQ=255)                                                           aaGTGATCATTGAACTCAATCACTATAACGATCCGCGCGTTGAAGAACTGGCGGAGATTGAG‑ATTCCTGGCGCGCCACCGCGACGCAATAGCGTGTCGATCTTACATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACGAACTTCCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGCTAACGTGGTCACg                                                                                                                                                                                  >  2:42950/1‑251 (MQ=255)                                                                      gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑CTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGACGCCGGCGTGGAAACCAACTTAACCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTga                                                                                                                                                                        >  2:298292/1‑249 (MQ=255)                                                                             aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCAc                                                                                                                                                                                   >  1:339110/1‑232 (MQ=255)                                                                             aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCAc                                                                                                                                                                                   <  2:339110/232‑1 (MQ=255)                                                                                    tCACGATCCGCGCTTTGCAGAACTGGTGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGAGCTCCCGTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGAGCCGAAAAAGATTGTCTCCGTCGTGTAAACCAGCTTTCCCGATGGCGGTAATATGCTGGATAAGGAAAATCCTATGTGGCAGCAGAGTGCCGAGAACGTGGTCACGTTTGTATTTCAGTAAATGGAGCAt                                                                                                                                                          <  2:26337/250‑1 (MQ=255)                                                                                        gATCCGCTCGTTGCAGAACTGGCGGATAT‑GTGGAGTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGAGCTTCCATGCAATTGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTTTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCTAGCAGATTGCCGATAACGGGGTCACGTTCTTATTGCAGTAAATTGCTCATGGGCg                                                                                                                                                     <  2:396978/251‑1 (MQ=255)                                                                                        gATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg                                                                                                                                                     >  1:274362/1‑251 (MQ=255)                                                                                                     cAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCCCCGCGGCGCAATAGCGGGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGGAACCAACTTACCCGACGCCGGTAATATGCTGGGTAAGCAAAATCCCATGTGCCAGAAGATTGCCGATAACGTGGACACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGa                                                                                                                                         >  2:59546/1‑250 (MQ=255)                                                                                                       taaCTGGCGGATATTGTG‑ATTCCTGGCGCGCCACTGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGAATCGCGGCGGGACCCGCTATGTGCAAATCGATACGACAAAGATTGTCGCCGTCGTGGAAAACAACTTTCCCGACGCCGGGAATATGCTGGATAAGCAAAATCCCATTAGCCAGCAGATTGCCGAAAACATGGTCAAGTTCTTATTTCAGGAAATGGCGCAGGGGCGTCTTCCGCCGGAAtt                                                                                                                                      >  2:274916/2‑251 (MQ=255)                                                                                                            ggCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGc                                                                                                                                 >  1:15192/1‑251 (MQ=255)                                                                                                              cGGATATTGTG‑ATTCCTGGCGCGCCGCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCg                                                                                                                               <  1:313794/251‑1 (MQ=255)                                                                                                               cggctatgttgaatCCTGGCGCGCCACCTCGGTGCCATAGCGTTTCGATCTTCCATGCAATGGATCTCGTCGGTACCCTCTATGTGCAAATCGATCCGAAAAAGATTGGCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGTATAAGCAAAACCCCATGTGCCAGCAGAGTGCCGATTACGTTTGCACGTTCTTATTGCAGTAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCg                                                                                                                               <  2:39923/238‑1 (MQ=255)                                                                                                                                                       cGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGGGGCTTGGGCAATATCAATAATGCTGTAATgg                                                                                       <  2:396278/251‑1 (MQ=255)                                                                                                                                                       cGTGTCGATCTGCCATGCAATGGATCGCGACGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGGGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATgg                                                                                       <  2:126829/251‑1 (MQ=255)                                                                                                                                                            cGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCCTGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGAGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGACAATATCAATAATGCGGTAAtacggcgg                                                                                  >  2:282773/1‑244 (MQ=255)                                                                                                                                                                   ccATGCAATGGATCGCGTAGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATAGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGAAATATCAATAATGCGGTAATGGCGCGTCTggggg                                                                           >  2:281797/1‑251 (MQ=255)                                                                                                                                                                                         aCCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGAAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGtt                                                     >  1:335338/1‑251 (MQ=255)                                                                                                                                                                                             gCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatg                                                 >  1:4389/1‑251 (MQ=255)                                                                                                                                                                                                   tgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTAt                                           >  1:328183/1‑251 (MQ=255)                                                                                                                                                                                                    gCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATTATGTAtt                                          <  2:51700/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaaaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTTATATGCTGGATAAGCAAAATCCCATGTGCCATCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACa                             <  1:78007/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaaaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGGAGTGCTAc                              >  2:19864/1‑250 (MQ=255)                                                                                                                                                                                                                 aaaaaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAACCGGTCATTTCTCCGTTTATGATGTATTCGGAAGAGCTaa                              >  1:221331/1‑249 (MQ=255)                                                                                                                                                                                                                            cGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtgg                  >  1:236339/1‑251 (MQ=255)                                                                                                                                                                                                                                    gggAAACCAACTTACCCGAGGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAGTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTGCTTCGTTTATTATGTAGTCGGAAGGGCTCCAGGAATCGGTGGTGTATTTa          <  2:333139/250‑1 (MQ=255)                                                                                                                                                                                                                                            aaCTTACCCGACGCCTGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAc  <  1:212127/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTG‑ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAC  >  NC_000913/3065210‑3065694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑