Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,472	G→A	L66L (CTG→TTG)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,472	0	G	A	95.8%	69.4 / ‑3.4	24	L66L (CTG→TTG)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (7/16);  total (7/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

AAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATT  >  NC_000913/3050241‑3050716                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     aagggTCAGCACGAACGGTAATTTTGATGCCGAAAGGTTCAGCGTGTTGGGTAATTCAGGTGAGGTTTTTTTCGCGGGTGGCTGAGTTAACAACGAGGCGTAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCTTCTATCACACCGCCAGATGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTGGGTGAGCTTCGCCACATCGGTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTg                                                                                                                                                                                                                                   <  1:35750/248‑1 (MQ=255)          caCGAACGGTCATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGATGTCTTTTTCGCGGGTGGAGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGGAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAg                                                                                                                                                                                                                           <  1:238486/250‑1 (MQ=255)          caCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGa                                                                                                                                                                                                                             >  1:115820/1‑248 (MQ=255)             gAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGAGGCTGCCGCGacgcacg                                                                                                                                                                                                                       >  1:234768/1‑245 (MQ=255)                  gTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGt                                                                                                                                                                                                                                          >  1:141547/1‑227 (MQ=255)                  gTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGt                                                                                                                                                                                                                                          <  2:141547/227‑1 (MQ=255)                     aTTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGt                                                                                                                                                                                                               <  2:153968/251‑1 (MQ=255)                         cGATGCCGAAAGGTTCAGCGTGGTGGTGAATCCATGATAGGTCTTTGTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCAGCTATCACACCGCCAGAGGCTTTCAACACCCCCGAGTAAATGGCTTTTCCGCTGTTGTTGTGCTTCGCCTCCGCGGTCGCCAGCATATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCat                                                                                                                                                                                                            <  2:203584/250‑1 (MQ=255)                                 aaaGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGTAAGAAATCTTCAGTAAAGTAGTAGCCGATGAGGGCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACGCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGacac                                                                                                                                                                                                   <  2:104720/251‑1 (MQ=255)                                     gTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGGTCGGCTGCCGCGAAGATCGACGATGGTCATATGGGACACATCa                                                                                                                                                                                               <  1:311229/251‑1 (MQ=255)                                                tGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGc                                                                                                                                                                                    >  1:44278/1‑251 (MQ=255)                                                        ccAGGCGAGGTCTTTTTCGCGGGTGGCGTAGTTAACAACGAGGCGTAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACCCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGTCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCTCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGAGCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGgta                                                                                                                                                                             <  1:196239/250‑1 (MQ=255)                                                                ggTCTTTTTCGCGGGTGGCGGAGTTAACAACGGTGCGGAAGAAATCTTCAGTAAAGTAGTAGCCGATGAGGGCATCTATCACACCGCCAGAGGCATTCAACATCCCCTAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCTTTCGCCAGCAGATAACGCAAAAACTCCCGGGGGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCa                                                                                                                                                                    <  1:163652/251‑1 (MQ=255)                                                                                                               tCAGTAAAGTAGTAGCCGATGAGGTCATCTAGCACTCCGCCAGAGGCATACAACATCCCCGAGTAAAGTGCTGTGCTGCTTTTGGGGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGTTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACATTATTGCAGCGGcatcatta                                                                                                                         <  2:249352/247‑3 (MQ=255)                                                                                                                   tAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCAGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGCTTTGCGAACCGTATTGCAGCGGCATCATCCAGCCGTgg                                                                                                                 >  2:29176/1‑251 (MQ=255)                                                                                                                                               cacaCCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTGTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCTGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGc                                                                                      <  2:286851/250‑1 (MQ=255)                                                                                                                                                                          cGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCtgtt                                                          <  1:151972/251‑1 (MQ=255)                                                                                                                                                                            aGTAAAGAGCTTTGCCGCTTTTGGTGCGCTTAGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGACGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTCCGAACCGCATGATGTTCGTCGATTTACGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCAACAAGCGAGCGCCGCAAAGCATGTGGTGTTCGAACAAAGGAGGCtggttg                                                        >  2:251534/1‑247 (MQ=255)                                                                                                                                                                                          ccGCTTTTGGTGAGCTTCGGCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTc                                          <  2:74701/251‑1 (MQ=255)                                                                                                                                                                                                        cTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCggg                            >  1:271181/1‑251 (MQ=255)                                                                                                                                                                                                                 aTCGTTCGCCAGCAGATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAAc                   <  1:171622/251‑1 (MQ=255)                                                                                                                                                                                                                              agATAACGCAAAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGa      <  1:71588/251‑1 (MQ=255)                                                                                                                                                                                                                               gATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGTGGCTGACAACTTTTTCATGGTGaa     <  2:383836/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaCGCAAAAACTTCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCTTATGTGACACATCAAACATGCCGGCATCGGTACGTACCGCATGATGTTCGGCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAAGCCACCATGCGAGCGCCGCAAAGCGTGTGTTGGTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAAtt  <  1:93655/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAACTCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATT  >  NC_000913/3050241‑3050716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑