Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,772	C→A	G155G (GGC→GGA)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,772	0	C	A	100.0%	99.7 / NA	31	G155G (GGC→GGA)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/15);  total (16/15)

GATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGA  >  NC_000913/2926535‑2927017                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCGTGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGTAGGTCACTCAAtt                                                                                                                                                                                                                                           <  2:296332/250‑1 (MQ=255)     aatcttccGGAAGAGGCTTTTGGCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCTGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACGCCGTCCACATCACTAACCTGGTCTTTTCCAGCTTGCGTAACGCGCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGa                                                                                                                                                                                                                                      <  2:177051/245‑1 (MQ=255)                                         agtctcgcTTGGCAGGTCAACTTGTTCGCGGTTCTGCGTGACATTCCCTTCGTGTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCTACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCATATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaaatc                                                                                                                                                                                                   <  2:322775/245‑3 (MQ=255)                                          ttCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGTGAGGTCACTCAATTAACGAAAACGAGTATGTGTATGCCCGTCGCGGCGGAAACCa                                                                                                                                                                                                  <  2:131053/250‑1 (MQ=255)                                                ctgtGCAGGACAACTTGATCGCGATCCTGCGTGACATTCTCTTCGTTTATGGGCAAATACATAACACCGTTCGTTTTACCACCCTGAATCTCGAAAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGAGCCAAATATGGTGGACTGCTGGGGAGGTCACTCAATTAACGAAAACGAGAATTTGTATGCCCGACGCGTCGAAAACCAACTGGGc                                                                                                                                                                                           >  2:394029/4‑251 (MQ=255)                                                          cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCTTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGc                                                                                                                                                                                 <  2:212749/251‑1 (MQ=255)                                                                       gTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGATTATTTGTTTGCCCGTCGCGTCGGAACCCAGCTGGGCCTGCGTGAGATGAATATCGGCAc                                                                                                                                                                    >  1:373310/1‑251 (MQ=255)                                                                         ccTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCg                                                                                                                                                                  <  2:83483/251‑1 (MQ=255)                                                                                gACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACGCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGGGAGCTGAATATCTGCACCGTCTGTgg                                                                                                                                                           <  2:382248/251‑1 (MQ=255)                                                                                  cagtctcGTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTACCATCCTGAATCTCGACAACTCCGTCCACAGCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATTGTGGTCTGCTGGGGAGGTCACTCAATTAGCGAAAACGAGTACTTGTATGCCCGTCGCTTCGTAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTc                                                                                                                                                         <  1:392268/248‑1 (MQ=255)                                                                                    ttCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCCCCGGCTGTGGTCCg                                                                                                                                                       >  1:138614/1‑251 (MQ=255)                                                                                     tctcTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTACACATCACTAACCTGGTCTTTTCAATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACAAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCgg                                                                                                                                                      >  2:177724/1‑251 (MQ=255)                                                                                                                    gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgctgc                                                                                                                       >  1:86401/1‑251 (MQ=255)                                                                                                                    gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATAGCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgctgc                                                                                                                       <  1:19737/251‑1 (MQ=255)                                                                                                                                  ccTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACAcgagc                                                                                                         >  1:323737/1‑248 (MQ=255)                                                                                                                                    tGAATCTCGACAATTCCGTCCACATCACTAACCTGGTCTTTTTCATCTTGCGTAACGCTCGCGCGCTGCATGAGCGTGAAGCCTCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATGAACGAAAACGAGTATTTGTATGCCCGTCGCGTCTGAAACCAGCTGGGCCTGCGGGAGCTGAAAATCTGCACCGGCTGTGGTCCGGGAGCTATGGATGGGCGGCTGAAAGGTGCTGCGGTGGGTCACgcgcgg                                                                                                       >  2:110630/1‑249 (MQ=255)                                                                                                                                             aCAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGTGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAAc                                                                                                                                                                                                    >  2:90399/1‑149 (MQ=255)                                                                                                                                             aCAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGTGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAAc                                                                                                                                                                                                    <  1:90399/149‑1 (MQ=255)                                                                                                                                              cAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGCCACGCGCAGCAGCGTTAc                                                                                              >  1:356097/1‑250 (MQ=255)                                                                                                                                                   ccGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGAc                                                                                        >  1:249559/1‑251 (MQ=255)                                                                                                                                                            tCACTAACCTGGACTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGGGGTCCGGGAGCGATGGGAGCGCCGCTGACAGGTGCTGCGGGCGGACACGCGCAGCAGCGTTACAAAGACAGTCGtttt                                                                               >  2:292629/1‑251 (MQ=255)                                                                                                                                                                                     gCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgtc                                                                                                                                                                                                          >  1:36502/1‑103 (MQ=255)                                                                                                                                                                                     gCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGGATTTTTATGCCcgtcgcgtc                                                                                                                                                                                                          <  2:36502/103‑1 (MQ=255)                                                                                                                                                                                       gTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATc                                                    >  1:60471/1‑251 (MQ=255)                                                                                                                                                                                                  gcgcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAAcc                                         >  1:308865/1‑251 (MQ=255)                                                                                                                                                                                                    gcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGc                                       >  2:103036/1‑251 (MQ=255)                                                                                                                                                                                                       gCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGAGGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAATACAGTCGTTTTATTGGTATTACAGAGCTGTCGATTATCGCCGCTGAACCGCCTa                                    <  2:249559/251‑1 (MQ=255)                                                                                                                                                                                                       gCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATGTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTa                                    <  2:138614/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaTATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAAt                  >  1:214366/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggggAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAGCCAGCTGGGCCGGCGTGAGCGGAATATCTGCACCGGCTGGGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGTACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACATAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATTCCAGa  <  2:383543/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggggAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGa  <  1:189734/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGA  >  NC_000913/2926535‑2927017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑