Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,243	A→G	T636A (ACG→GCG)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,243	0	A	G	100.0%	155.4 / NA	49	T636A (ACG→GCG)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/20);  total (29/20)

GCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATA  >  NC_000913/2856020‑2856479                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              gctgGCGAAAGCCTTTACCTTCCAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATg                                                                                                                                                                                                                    >  1:124376/1‑251 (MQ=255)  ctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGAGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATg                                                                                                                                                                                                                    >  1:40912/1‑250 (MQ=255)    ggCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTTCGCTCCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAat                                                                                                                                                                                                                 <  1:175012/251‑1 (MQ=255)                tACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCAGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCAGCCTGCCGCAGCGGGTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtg                                                                                                                                                                                                     >  2:1846/1‑251 (MQ=255)                 aCCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtgt                                                                                                                                                                                                    <  1:357941/251‑1 (MQ=255)                  ccTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtgtg                                                                                                                                                                                                   <  1:91120/251‑1 (MQ=255)                                 gtcgtcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaaagaa                                                                                                                                                                                    <  1:140203/251‑1 (MQ=255)                                               aaTATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGCAACCAACGGCGTGGt                                                                                                                                                                      >  2:315675/1‑251 (MQ=255)                                                 tatcgatatcgtttcTGCAGAGGCGCGGCGCACCTTGAGCCACATGGCGGGGATGGGTAACGCGCGCGGCCTGGAAAACGACATTGAGCGCGCGGTATTGATAACAAGCGGTACCGTGCTGCAGATGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCAGCCGCAGCGGTTGTCTCCCTGGCGGGCGAAGGTGAATATCCGTTGCTTGTGCGCGTGCTGAAAGAACCCGACGGCGTGGTTg                                                                                                                                                                    >  2:138856/15‑251 (MQ=255)                                                    cGACAGCCTTACTGCCGAGACGCTGCGCACCTTGCGCCCCATGGAGTGGCCGGGTAACGTACGCGCACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCg                                                                                                                                                                 >  2:164259/1‑251 (MQ=255)                                                      aCAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAACCGTCATTGAGCGCGCGGTATTGCTAACCCGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGCTGACTATCGGTTGATTGTGCGCGTGCTGAACGAACCCAACGGCGTGGTTGCCggg                                                                                                                                                               >  1:31948/1‑251 (MQ=255)                                                         gCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCt                                                                                                                                                            <  1:238265/251‑1 (MQ=255)                                                              ccTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGAAACGTAAGCGAACTGGAAAACGTCATTGAGCGCGAGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAgg                                                                                                                                                       >  1:399918/1‑251 (MQ=255)                                                                      gaCGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGCGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGTCCTAAAGGCCCTgagc                                                                                                                                               >  2:208722/1‑248 (MQ=255)                                                                        cGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCa                                                                                                                                              >  1:300026/1‑250 (MQ=255)                                                                                 ccTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGGCTgggg                                                                                                                                    <  1:315675/251‑1 (MQ=255)                                                                                  cTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAGCGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGc                                                                                                                                   >  1:307351/1‑251 (MQ=255)                                                                                         aaCATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACg                                                                                                                            <  1:261007/251‑1 (MQ=255)                                                                                                      ggggTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCctgctg                                                                                                               <  1:133186/250‑1 (MQ=255)                                                                                                         gTAACGTACGCGACCTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGCTATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCTGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTcc                                                                                                            >  1:78595/1‑250 (MQ=255)                                                                                                         gTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCAAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTc                                                                                                             >  1:191037/1‑250 (MQ=255)                                                                                                            aCGTAGGCGAACTGGAAGACGCCAGTCAGCACGCCGCACTGCTACCACGCGGTCACGGGCTGCAGCTGTCCTTGCCCGCTCTTGTT‑TTACCGGAACCTGAAACGCCGCCCGCCGAAGCGGTTGTCGCCCAGGAGGGCCCAGCTGAATATCAGTTGATTGTGCGCGCGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAACGGCGCTGCGCAACGTCTGGGGATGAAACGCCCGACCCCGCTGTCACgg                                                                                                         >  2:182252/1‑251 (MQ=255)                                                                                                                    gAACTGGAAAACGTACTTCAGCGCCCGGAATTGCTAACAAGCGGTAACGTGCTGCAGCTGTCCTTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCCGTTGCTTGTGCGCGTGCTGAAAGACACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACACTGATGTCACGGATGAAGCg                                                                                                 >  2:43070/1‑251 (MQ=255)                                                                                                                     aaCTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCgg                                                                                                >  1:299978/1‑251 (MQ=255)                                                                                                                         ggcaaaCGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCGGAAACGCACGACCCTGCTGTCACGGTCGAAGCGGCt                                                                                              >  2:312876/4‑249 (MQ=255)                                                                                                                           aaaaCGTCATTGAGCCCGCGGACTTTCTAACACGTGGTAACTTGCTGCAGCGTTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGa                                                                                          <  2:220836/251‑1 (MQ=255)                                                                                                                            aaaCGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGATGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGa                                                                                          >  2:376997/1‑250 (MQ=255)                                                                                                                              aCGTCATTGAGCGCGCGTTATTGTTACCTCGCGTTATCGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTTCCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCTACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAAtt                                                                                       <  1:340185/251‑1 (MQ=255)                                                                                                                                gTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTg                                                                                      >  1:14870/1‑250 (MQ=255)                                                                                                                                     tgggcggtcgGTATTGCTAACACGCGGTAACGTGCGGCAGCTTTCCTGGCCAGGTTTTGTT‑TGACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACACACGACCGTGCTGTCACGTATGAAGCGTCTGGTAAGTG‑ATaaa                                                                                <  2:256999/242‑1 (MQ=255)                                                                                                                                     tGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAAATATTGTT‑TTACAGGCACCTGAAACGCCGCCTGCCGCAGCGGTTGTCACCCTGGAGGGCGAAGATGCATAACAGTTGATTGTCCGCGCGCTGAAAGAAACCAACGGCGTGGATGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAAAGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATaaa                                                                                >  1:397033/1‑250 (MQ=255)                                                                                                                                                tATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACACTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGAtt                                                                    >  1:319140/1‑251 (MQ=255)                                                                                                                                                 aTTGCTAACACGCGGTAACGTGCGGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGAtt                                                                    <  2:70677/250‑1 (MQ=255)                                                                                                                                                    gCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAAc                                                                >  1:286339/1‑251 (MQ=255)                                                                                                                                                       aaCACGCGGTAACGTGCTGCAGCGGGCATGGGCAGATATTGTT‑TTACCGGAGCCTGAACCGCCGCCGGCCGCAGCGGTTGTCGCCGTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGGGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCGTAAAGTCGCTGCGGAACGTCTGGGGTTGAATCGCACGACCCTGCTGTCACGGATGAAGGTGC‑GGGAAATGTGTAAATCGGCATTgtggtaactgc                                                             <  2:237230/251‑11 (MQ=255)                                                                                                                                                        acacGCGGTAACGTGCTGCAGCTGTTATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCa                                                            <  2:163015/251‑1 (MQ=255)                                                                                                                                                            gcgttaaagTGCTGCAGCTGTCAGTGCCAGATAT‑GTTCTTACCGGAACCTGAAACGCACCCTGCCGCAGCGGTTGTCGACCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGATGAAAGAAACCAACGGCGTGGGTTCCGGGCCTAAAGGCGATGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACTGATGAGGCGGCTGGGAAATG‑ATAAATCGGCAGTTATTTATCTGCAAAtt                                                        <  2:138003/243‑1 (MQ=255)                                                                                                                                                               gTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGCGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGcc                                                     >  1:187963/1‑251 (MQ=255)                                                                                                                                                               gTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGCTTTAACTGCAAATTGcc                                                     >  2:260547/1‑251 (MQ=255)                                                                                                                                                                   cgggctgcAGCTGTCATTGCCAGATATGGTT‑TTACCGGAACCTAAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCGGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAAGTGCCGGAc                                                 <  2:39048/248‑1 (MQ=255)                                                                                                                                                                           aGCTGTCATTGCCAGATATTGTT‑TTACCGGCACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGAGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCAAGGATGGAGCGGATGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGc                                         >  2:132193/1‑251 (MQ=255)                                                                                                                                                                                tCATTGCCAGGTATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGCGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGCTGAAGCGGCTGGGAATTG‑TTAAATAGGCCTTGATTTTACTGCAAATTGCCGGGCAGATCTGCCCGTc                                    >  2:40267/1‑251 (MQ=255)                                                                                                                                                                                 cATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTcc                                   <  1:313364/251‑1 (MQ=255)                                                                                                                                                                                         gATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACt                           <  1:164259/251‑1 (MQ=255)                                                                                                                                                                                                    gaCCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAg                  <  2:313620/249‑1 (MQ=255)                                                                                                                                                                                                       cGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGACTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGACTGGGGCTGAAACGTACGACCCTGCTGTCACGGATGAAGCGGCTGGGGATTT‑ATAAATAGGCATCGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCAGGCctactaatcctgcgggtt              >  2:218691/1‑239 (MQ=255)                                                                                                                                                                                                                   ctccgccgGCCGCAGCGGTTGTCGCCCTGGAGGGCGCAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGACGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTCACTGCCAATTGCCGGCAAGATCTGCCTGTCCGGCACACTATTCCTGAGGTTTTTTCGGACGatc  >  2:4067/3‑250 (MQ=255)                                                                                                                                                                                                                    gccgccTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGat   <  2:388930/249‑1 (MQ=255)                                                                                                                                                                                                                    gccgccTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGat   >  1:388930/1‑249 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              GCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTT‑TTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTG‑ATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATA  >  NC_000913/2856020‑2856479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑